Cryo-EM structure of Escherichia coli RNAP polymerase bound with TraR in conformation I
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6n57
Q-score: 0.447
Chen J, Gopalkrishnan S, Chiu C, Chen AY, Campbell EA, Gourse RL, Ross W, Darst SA
eLife (2019) 8 [ Pubmed: 31841111 DOI: doi:10.7554/eLife.49375 ]
- Magnesium ion (24 Da, Ligand)
- Protein trar (8 kDa, Protein from Escherichia coli)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Escherichia coli sigma70-holoenzyme with trar in conformation i (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Cryo-EM structure of Escherichia coli RNAP polymerase bound with TraR in conformation II
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 6n58
Q-score: 0.443
Chen J, Gopalkrishnan S, Chiu C, Chen AY, Campbell EA, Gourse RL, Ross W, Darst SA
eLife (2019) 8 [ Pubmed: 31841111 DOI: doi:10.7554/eLife.49375 ]
- Magnesium ion (24 Da, Ligand)
- Protein trar (8 kDa, Protein from Escherichia coli)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Escherichia coli sigma70-holoenzyme with trar in conformation ii (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
CryoEM structure of SigmaS-transcription initiation complex with activator Crl
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 6omf
Q-score: 0.463
Cartagena AJ, Banta AB, Sathyan N, Ross W, Gourse RL, Campbell EA, Darst SA
PNAS (2019) 116 pp. 18923-18927 [ DOI: doi:10.1073/pnas.1910827116 Pubmed: 31484766 ]
- Dna-directed rna polymerase subunit beta (Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (Protein from Escherichia coli)
- Cryoem structure of sigmas-transcription initiation complex with activator crl (523 kDa, Complex from Salmonella enterica subsp. enterica serovar Typhimurium)
- Dna-directed rna polymerase subunit sigma-s (Protein from Salmonella enterica subsp. enterica serovar Typhimurium)
- Dna-directed rna polymerase subunit alpha (Protein from Escherichia coli)
- Template dna strand (DNA from Escherichia coli)
- Non-template dna strand (DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (Protein from Escherichia coli)
- Crl (Protein from Salmonella enterica subsp. enterica serovar Typhimurium)