Serotonin 4 (5-HT4) receptor-Gs-Nb35 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7xt8
Q-score: 0.533
Huang S, Xu P, Shen DD, Simon IA, Mao C, Tan Y, Zhang H, Harpsoe K, Li H, Zhang Y, You C, Yu X, Jiang Y, Zhang Y, Gloriam DE, Xu HE
Mol Cell (2022) 82 pp. 2681-2695.e6 [ Pubmed: 35714614 DOI: doi:10.1016/j.molcel.2022.05.031 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Serotonin 4 (5-ht4) receptor-gs-nb35 complex (Complex from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Soluble cytochrome b562,5-hydroxytryptamine receptor 4 (59 kDa, Protein from Homo sapiens)
- Serotonin (176 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Serotonin 4 (5-HT4) receptor-Gs complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7xt9
Q-score: 0.521
Huang S, Xu P, Shen DD, Simon IA, Mao C, Tan Y, Zhang H, Harpsoe K, Li H, Zhang Y, You C, Yu X, Jiang Y, Zhang Y, Gloriam DE, Xu HE
Mol Cell (2022) 82 pp. 2681-2695.e6 [ Pubmed: 35714614 DOI: doi:10.1016/j.molcel.2022.05.031 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,5-hydroxytryptamine receptor 4 (59 kDa, Protein from Homo sapiens)
- Serotonin (176 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Serotonin 4 (5-ht4) receptor-gs complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Serotonin 4 (5-HT4) receptor-Gi-scFv16 complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7xta
Q-score: 0.426
Huang S, Xu P, Shen DD, Simon IA, Mao C, Tan Y, Zhang H, Harpsoe K, Li H, Zhang Y, You C, Yu X, Jiang Y, Zhang Y, Gloriam DE, Xu HE
Mol Cell (2022) 82 pp. 2681-2695.e6 [ Pubmed: 35714614 DOI: doi:10.1016/j.molcel.2022.05.031 ]
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Serotonin 4 (5-ht4) receptor-gi-scfv16 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,5-hydroxytryptamine receptor 4 (59 kDa, Protein from Homo sapiens)
- Serotonin (176 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Serotonin 6 (5-HT6) receptor-Gs-Nb35 complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7xtb
Q-score: 0.46
Huang S, Xu P, Shen DD, Simon IA, Mao C, Tan Y, Zhang H, Harpsoe K, Li H, Zhang Y, You C, Yu X, Jiang Y, Zhang Y, Gloriam DE, Xu HE
Mol Cell (2022) 82 pp. 2681-2695.e6 [ Pubmed: 35714614 DOI: doi:10.1016/j.molcel.2022.05.031 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Serotonin 6 (5-ht6) receptor-gs-nb35 complex (Complex from Homo sapiens)
- Soluble cytochrome b562,5-hydroxytryptamine receptor 6 (63 kDa, Protein from Homo sapiens)
- Serotonin (176 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Serotonin 7 (5-HT7) receptor-Gs-Nb35 complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7xtc
Q-score: 0.455
Huang S, Xu P, Shen DD, Simon IA, Mao C, Tan Y, Zhang H, Harpsoe K, Li H, Zhang Y, You C, Yu X, Jiang Y, Zhang Y, Gloriam DE, Xu HE
Mol Cell (2022) 82 pp. 2681-2695.e6 [ Pubmed: 35714614 DOI: doi:10.1016/j.molcel.2022.05.031 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Cholesterol (386 Da, Ligand)
- Soluble cytochrome b562,5-hydroxytryptamine receptor 7 (64 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Serotonin 7 (5-ht7) receptor-gs-nb35 complex (Complex from Homo sapiens)
- 3-(2-azanylethyl)-1h-indole-5-carboxamide (203 Da, Ligand)
Cryo-EM structure of a class A orphan GPCR in complex with Gi
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7vug
Q-score: 0.445
Zhou Y, Daver H, Trapkov B, Wu L, Wu M, Harpsoe K, Gentry PR, Liu K, Larionova M, Liu J, Chen N, Brauner-Osborne H, Gloriam DE, Hua T, Liu ZJ
Cell Res (2022) 32 pp. 210-213 [ DOI: doi:10.1038/s41422-021-00591-w Pubmed: 34916631 ]
- Scfv16 (27 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Chimera of endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (60 kDa, Protein from Homo sapiens)
- Scfv16 (Complex)
- Ternary complex of ligand, gpcr and g protein (180 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Endo-1,4-beta-xylanase,probable g-protein coupled receptor 139 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Bacillus circulans)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 3-chloranyl-n-[2-oxidanylidene-2-[[(1s)-1-phenylethyl]amino]ethyl]benzamide (316 Da, Ligand)
Cryo-EM structure of a class A orphan GPCR
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 7vuh
Q-score: 0.409
Zhou Y, Daver H, Trapkov B, Wu L, Wu M, Harpsoe K, Gentry PR, Liu K, Larionova M, Liu J, Chen N, Brauner-Osborne H, Gloriam DE, Hua T, Liu ZJ
Cell Res (2022) 32 pp. 210-213 [ DOI: doi:10.1038/s41422-021-00591-w Pubmed: 34916631 ]
- Nanobody 35 (17 kDa, Protein from Homo sapiens)
- 3-chloranyl-n-[2-oxidanylidene-2-[[(1s)-1-phenylethyl]amino]ethyl]benzamide (316 Da, Ligand)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Nanobody 35 (Complex from Bacillus circulans)
- Chimera of endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (60 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Ternary complex of ligand, gpcr and g protein (150 kDa, Complex from Homo sapiens)
- Endo-1,4-beta-xylanase,probable g-protein coupled receptor 139 (Complex)
Cryo-EM structure of a class A orphan GPCR
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7vui
Q-score: 0.389
Zhou Y, Daver H, Trapkov B, Wu L, Wu M, Harpsoe K, Gentry PR, Liu K, Larionova M, Liu J, Chen N, Brauner-Osborne H, Gloriam DE, Hua T, Liu ZJ
Cell Res (2022) 32 pp. 210-213 [ DOI: doi:10.1038/s41422-021-00591-w Pubmed: 34916631 ]
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Homo sapiens)
- 3-chloranyl-n-[2-oxidanylidene-2-[[(1s)-1-phenylethyl]amino]ethyl]benzamide (316 Da, Ligand)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Chimera of endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (60 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Nanobody 35 (Complex from Bacillus circulans)
- Magnesium ion (24 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Ternary complex of ligand, gpcr and g protein (150 kDa, Complex from Homo sapiens)
- Endo-1,4-beta-xylanase,probable g-protein coupled receptor 139 (Complex)
Cryo-EM structure of a class A orphan GPCR
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7vuj
Q-score: 0.212
Zhou Y, Daver H, Trapkov B, Wu L, Wu M, Harpsoe K, Gentry PR, Liu K, Larionova M, Liu J, Chen N, Brauner-Osborne H, Gloriam DE, Hua T, Liu ZJ
Cell Res (2022) 32 pp. 210-213 [ DOI: doi:10.1038/s41422-021-00591-w Pubmed: 34916631 ]
- Nanobody 35 (17 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Chimera endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (Complex)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Nanobody 35 (Complex from Bacillus circulans)
- Magnesium ion (24 Da, Ligand)
- Chimera of endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (60 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Ternary complex of ligand, gpcr and g protein (150 kDa, Complex from Homo sapiens)
- 3-chloranyl-n-[2-oxidanylidene-2-[[(1s)-1-phenylethyl]amino]ethyl]benzamide (316 Da, Ligand)
Serotonin 1D (5-HT1D) receptor-Gi protein complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7e32
Q-score: 0.495
Xu P, Huang S, Zhang H, Mao C, Zhou XE, Cheng X, Simon IA, Shen DD, Yen HY, Robinson CV, Harpsoe K, Svensson B, Guo J, Jiang H, Gloriam DE, Melcher K, Jiang Y, Zhang Y, Xu HE
Nature (2021) 592 pp. 469-473 [ Pubmed: 33762731 DOI: doi:10.1038/s41586-021-03376-8 ]
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,5-hydroxytryptamine receptor 1d (57 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Serotonin (176 Da, Ligand)
- Serotonin 1d (5-ht1d) receptor-gi protein complex (Complex)
- Scfv16 (Complex from Mus musculus)
- Receptor-gi protein (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)