Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8ww2
Q-score: 0.577
Xiong Y, Xu Z, Li X, Wang Y, Zhao J, Wang N, Duan Y, Xia R, Han Z, Qian Y, Liang J, Zhang A, Guo C, Inoue A, Xia Y, Chen Z, He Y
Cell Res (2024) 34 pp. 232-244 [ DOI: doi:10.1038/s41422-024-00932-5 Pubmed: 38287117 ]
- Water (18 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Nb35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Oleic acid (282 Da, Ligand)
- G-protein coupled receptor 3 (35 kDa, Protein from Homo sapiens)
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8kae
Q-score: 0.509
Duan Y, Leong NCP, Zhao J, Zhang Y, Nguyen DT, Ha HTT, Wang N, Xia R, Xu Z, Ma Z, Qian Y, Yin H, Zhu X, Zhang A, Guo C, Xia Y, Nguyen LN, He Y
Cell Res (2024) 34 pp. 177-180 [ DOI: doi:10.1038/s41422-023-00913-0 Pubmed: 38123825 ]
- Nbfab chain l (23 kDa, Protein from synthetic construct)
- Tc-nb 8 (14 kDa, Protein from Lama glama)
- 3-[3-(4-decylphenyl)-1,2,4-oxadiazol-5-yl]propan-1-amine (343 Da, Ligand)
- Spns2 nanobody complex (Complex from Lama glama)
- Nbfab-h chain (26 kDa, Protein from synthetic construct)
- Sphingosine-1-phosphate transporter spns2 (58 kDa, Protein from Homo sapiens)
- Anti-fab nb (13 kDa, Protein from Lama glama)
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 7yub
Q-score: 0.513
Duan Y, Leong NCP, Zhao J, Zhang Y, Nguyen DT, Ha HTT, Wang N, Xia R, Xu Z, Ma Z, Qian Y, Yin H, Zhu X, Zhang A, Guo C, Xia Y, Nguyen LN, He Y
Cell Res (2024) 34 pp. 177-180 [ DOI: doi:10.1038/s41422-023-00913-0 Pubmed: 38123825 ]
- (2s,3r,4e)-2-amino-3-hydroxyoctadec-4-en-1-yl dihydrogen phosphate (379 Da, Ligand)
- Nbfab-h-chain (26 kDa, Protein from synthetic construct)
- Nbfab-l-chain (23 kDa, Protein from synthetic construct)
- Tc-nb8 (14 kDa, Protein from Lama glama)
- Spns2 nanobody complex (Complex from Lama glama)
- Sphingosine-1-phosphate transporter spns2 (58 kDa, Protein from Homo sapiens)
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 7yud
Q-score: 0.53
Duan Y, Leong NCP, Zhao J, Zhang Y, Nguyen DT, Ha HTT, Wang N, Xia R, Xu Z, Ma Z, Qian Y, Yin H, Zhu X, Zhang A, Guo C, Xia Y, Nguyen LN, He Y
Cell Res (2024) 34 pp. 177-180 [ DOI: doi:10.1038/s41422-023-00913-0 Pubmed: 38123825 ]
- Nbfab-h-chain (26 kDa, Protein from synthetic construct)
- Tc-nb8 (14 kDa, Protein from Lama glama)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Spns2 nanobody complex (Complex from Lama glama)
- (2~{s})-2-azanyl-4-(4-octylphenyl)-2-[[oxidanyl-bis(oxidanylidene)-$l^{6}-phosphanyl]oxymethyl]butan-1-ol (387 Da, Ligand)
- Sphingosine-1-phosphate transporter spns2 (58 kDa, Protein from Homo sapiens)
- Nbfab l-chain (23 kDa, Protein from synthetic construct)
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 7yuf
Q-score: 0.458
Duan Y, Leong NCP, Zhao J, Zhang Y, Nguyen DT, Ha HTT, Wang N, Xia R, Xu Z, Ma Z, Qian Y, Yin H, Zhu X, Zhang A, Guo C, Xia Y, Nguyen LN, He Y
Cell Res (2024) 34 pp. 177-180 [ DOI: doi:10.1038/s41422-023-00913-0 Pubmed: 38123825 ]
- Antifab nanobody (13 kDa, Protein from Lama glama)
- Tc-nb8 (14 kDa, Protein from Lama glama)
- Spns2 nanobody complex (Complex from Lama glama)
- Nbfab h-chain (26 kDa, Protein from synthetic construct)
- Sphingosine-1-phosphate transporter spns2 (58 kDa, Protein from Homo sapiens)
- Nbfab l-chain (23 kDa, Protein from synthetic construct)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ydh
Q-score: 0.437
Wang N, Qian Y, Xia R, Zhu X, Xiong Y, Zhang A, Guo C, He Y
Cell Chem Biol (2023) 30 pp. 1343-1353.e5 [ Pubmed: 37673067 DOI: doi:10.1016/j.chembiol.2023.08.003 ]
- Adhesion g protein-coupled receptor e5 subunit beta (34 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G protein subunit 13 (gi2-mini-g13 chimera) (26 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (130 kDa, Complex from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 7ydm
Q-score: 0.54
Wang N, Qian Y, Xia R, Zhu X, Xiong Y, Zhang A, Guo C, He Y
Cell Chem Biol (2023) 30 pp. 1343-1353.e5 [ Pubmed: 37673067 DOI: doi:10.1016/j.chembiol.2023.08.003 ]
- Adhesion g protein-coupled receptor e5 subunit beta (34 kDa, Protein from Homo sapiens)
- Nb35 (Complex)
- Engineered mini-gaq (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (130 kDa, Complex from Homo sapiens)
- Gpcr/g-protein (Complex from Lama glama)
- Nb35 (17 kDa, Protein from Lama glama)
Cryo-EM structure of CD97/miniGs complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ydp
Q-score: 0.357
Wang N, Qian Y, Xia R, Zhu X, Xiong Y, Zhang A, Guo C, He Y
Cell Chem Biol (2023) 30 pp. 1343-1353.e5 [ Pubmed: 37673067 DOI: doi:10.1016/j.chembiol.2023.08.003 ]
- Adhesion g protein-coupled receptor e5 subunit beta (34 kDa, Protein from Homo sapiens)
- Nb35 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Engineered minigas (41 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (130 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gpcr/g-protein (Complex from Lama glama)
- Nb35 (17 kDa, Protein from Lama glama)
DHA-bound FFAR4 in complex with Gs
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 8h4i
Q-score: 0.574
Yin H, Inoue A, Ma Z, Zhu X, Xia R, Xu Z, Wang N, Duan Y, Zhang A, Guo C, He Y
Cell Res (2023) 33 pp. 644-647 [ Pubmed: 37286793 DOI: doi:10.1038/s41422-023-00835-x ]
- Docosa-4,7,10,13,16,19-hexaenoic acid (328 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Engineered mini galpha-s subunit (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Lama glama)
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Free fatty acid receptor 4 (40 kDa, Protein from Homo sapiens)
GW9508-bound FFAR4 in complex with Gq
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8h4k
Q-score: 0.545
Yin H, Inoue A, Ma Z, Zhu X, Xia R, Xu Z, Wang N, Duan Y, Zhang A, Guo C, He Y
Cell Res (2023) 33 pp. 644-647 [ Pubmed: 37286793 DOI: doi:10.1038/s41422-023-00835-x ]
- 3-(4-{[(3-phenoxyphenyl)methyl]amino}phenyl)propanoic acid (347 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Lama glama)
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Free fatty acid receptor 4 (40 kDa, Protein from Homo sapiens)
- Engineered mini galpha-q subunit (41 kDa, Protein from Homo sapiens)