Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 7x10
Q-score: 0.465
Qian Y, Ma Z, Liu C, Li X, Zhu X, Wang N, Xu Z, Xia R, Liang J, Duan Y, Yin H, Xiong Y, Zhang A, Guo C, Chen Z, Huang Z, He Y
Mol Cell (2022) 82 pp. 4340-4352.e6 [ Pubmed: 36309016 DOI: doi:10.1016/j.molcel.2022.10.009 ]
- Isoform 3 of adhesion g protein-coupled receptor l3 (172 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Engineered g alpha 12 subunit (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (Complex from Homo sapiens)
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 7wy5
Q-score: 0.514
Qian Y, Ma Z, Liu C, Li X, Zhu X, Wang N, Xu Z, Xia R, Liang J, Duan Y, Yin H, Xiong Y, Zhang A, Guo C, Chen Z, Huang Z, He Y
Mol Cell (2022) 82 pp. 4340-4352.e6 [ Pubmed: 36309016 DOI: doi:10.1016/j.molcel.2022.10.009 ]
- Isoform 3 of adhesion g protein-coupled receptor l3 (172 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Engineered mini g alpha q subunit (41 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Homo sapiens)
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 7wy8
Q-score: 0.504
Qian Y, Ma Z, Liu C, Li X, Zhu X, Wang N, Xu Z, Xia R, Liang J, Duan Y, Yin H, Xiong Y, Zhang A, Guo C, Chen Z, Huang Z, He Y
Mol Cell (2022) 82 pp. 4340-4352.e6 [ Pubmed: 36309016 DOI: doi:10.1016/j.molcel.2022.10.009 ]
- Nb35 (17 kDa, Protein from Lama glama)
- Isoform 3 of adhesion g protein-coupled receptor l3 (172 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nb35 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gpcr/g-protein (Complex from Lama glama)
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Engineered mini g alpha s subunit (41 kDa, Protein from Homo sapiens)
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 7wyb
Q-score: 0.352
Qian Y, Ma Z, Liu C, Li X, Zhu X, Wang N, Xu Z, Xia R, Liang J, Duan Y, Yin H, Xiong Y, Zhang A, Guo C, Chen Z, Huang Z, He Y
Mol Cell (2022) 82 pp. 4340-4352.e6 [ Pubmed: 36309016 DOI: doi:10.1016/j.molcel.2022.10.009 ]
- Gpcr/g-protein complex (Complex)
- Isoform 3 of adhesion g protein-coupled receptor l3 (172 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Adgrl3 (Complex from Mus musculus)
Cryo-EM structure of Dnf1 from Saccharomyces cerevisiae in 90PS with beryllium fluoride (E2P state)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7drx
Q-score: 0.556
Cell Rep (2022) 38 pp. 110518-110518 [ DOI: doi:10.1016/j.celrep.2022.110518 Pubmed: 35294892 ]
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Dnf1_lem3 complex (Complex from Saccharomyces cerevisiae S288C)
- Phospholipid-transporting atpase dnf1 (178 kDa, Protein from Saccharomyces cerevisiae S288C)
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae S288C)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
Cryo-EM structure of Dnf1 from Saccharomyces cerevisiae in 90PS with AMPPCP (E1-ATP state)
Resolution: 3.67 Å
EM Method: Single-particle
Fitted PDBs: 7dsh
Q-score: 0.428
Cell Rep (2022) 38 pp. 110518-110518 [ DOI: doi:10.1016/j.celrep.2022.110518 Pubmed: 35294892 ]
- Magnesium ion (24 Da, Ligand)
- Dnf1_lem3 complex (Complex from Saccharomyces cerevisiae S288C)
- Phospholipid-transporting atpase dnf1 (178 kDa, Protein from Saccharomyces cerevisiae S288C)
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 7dsi
Q-score: 0.422
Cell Rep (2022) 38 pp. 110518-110518 [ DOI: doi:10.1016/j.celrep.2022.110518 Pubmed: 35294892 ]
- Magnesium ion (24 Da, Ligand)
- Dnf1_lem3 complex (Complex from Saccharomyces cerevisiae S288C)
- Phospholipid-transporting atpase dnf1 (178 kDa, Protein from Saccharomyces cerevisiae S288C)
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae S288C)
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)
Resolution: 3.81 Å
EM Method: Single-particle
Fitted PDBs: 7f7f
Q-score: 0.379
Cell Rep (2022) 38 pp. 110518-110518 [ DOI: doi:10.1016/j.celrep.2022.110518 Pubmed: 35294892 ]
- Dnf1_lem3 complex (Complex from Saccharomyces cerevisiae S288C)
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae S288C)
- Phospholipid-transporting atpase dnf1 (178 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7whv
Q-score: 0.573
Cell Rep (2022) 38 pp. 110518-110518 [ DOI: doi:10.1016/j.celrep.2022.110518 Pubmed: 35294892 ]
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Dnf1_lem3 complex (Complex from Saccharomyces cerevisiae S288C)
- Phospholipid-transporting atpase dnf1 (178 kDa, Protein from Saccharomyces cerevisiae S288C)
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae S288C)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
Cryo-EM structure of Dnf1 from Saccharomyces cerevisiae in detergent with AMPPCP (E1-ATP state)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7whw
Q-score: 0.437
Cell Rep (2022) 38 pp. 110518-110518 [ DOI: doi:10.1016/j.celrep.2022.110518 Pubmed: 35294892 ]
- Magnesium ion (24 Da, Ligand)
- Dnf1_lem3 complex (Complex from Saccharomyces cerevisiae S288C)
- Phospholipid-transporting atpase dnf1 (178 kDa, Protein from Saccharomyces cerevisiae S288C)
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae S288C)
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)