cryo-EM structure of a nanobody bound heliorhodopsin
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 9ljj
Q-score: 0.531
Xia R, Sun M, Lu Y, Wang N, Zhang A, Guo C, Xu Z, Cai X, He Y
Biochem Biophys Res Commun (2025) 750 pp. 151398-151398 [ Pubmed: 39889627 DOI: doi:10.1016/j.bbrc.2025.151398 ]
- 1,2-dilauroyl-sn-glycero-3-phosphate (535 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Nanobody b4 (13 kDa, Protein from Vicugna pacos)
- Heliorhodopsin (28 kDa, Protein from Escherichia coli)
- Retinal (284 Da, Ligand)
- Dimer complex (Complex from Escherichia coli)
- Acetate ion (59 Da, Ligand)
LPA-bound LPAR6 in complex with miniGq
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 9itb
Q-score: 0.576
Duan Y, Xu Z, Hao B, Zhang A, Guo C, He Y
PNAS (2025) 122 pp. e2415426122-e2415426122 [ Pubmed: 39847322 DOI: doi:10.1073/pnas.2415426122 ]
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Lysophosphatidic acid receptor 6 (33 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Engineered minigaq (41 kDa, Protein from Homo sapiens)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl (9e)-octadec-9-enoate (436 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Lama glama)
- Cholesterol (386 Da, Ligand)
LPA-bound LPAR6 in complex with miniG13
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 9ite
Q-score: 0.558
Duan Y, Xu Z, Hao B, Zhang A, Guo C, He Y
PNAS (2025) 122 pp. e2415426122-e2415426122 [ Pubmed: 39847322 DOI: doi:10.1073/pnas.2415426122 ]
- Lysophosphatidic acid receptor 6 (34 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Engineered miniga13 (26 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl (9e)-octadec-9-enoate (436 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
CryoET of VSV incubated with liposomes at pH 5.5
Milojevic L, Si Z, Xia X, Chen L, He Y, Tang S, Luo M, Zhou ZH
Sci Adv (2024) 10 pp. eadn8579-eadn8579 [ Pubmed: 39630917 DOI: doi:10.1126/sciadv.adn8579 ]
Qian Y, Ma Z, Xu Z, Duan Y, Xiong Y, Xia R, Zhu X, Zhang Z, Tian X, Yin H, Liu J, Song J, Lu Y, Zhang A, Guo C, Jin L, Kim WJ, Ke J, Xu F, Huang Z, He Y
Nat Commun (2024) 15 pp. 7644-7644 [ Pubmed: 39223191 DOI: doi:10.1038/s41467-024-52174-z ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- Frizzled-4 (60 kDa, Protein from Homo sapiens)
- Segment polarity protein dishevelled homolog dvl-2 (79 kDa, Protein from Homo sapiens)
- Gpcr complex (450 kDa, Complex from Homo sapiens)
Fzd4/DEP complex (local refined)
Qian Y, Ma Z, Xu Z, Duan Y, Xiong Y, Xia R, Zhu X, Zhang Z, Tian X, Yin H, Liu J, Song J, Lu Y, Zhang A, Guo C, Jin L, Kim WJ, Ke J, Xu F, Huang Z, He Y
Nat Commun (2024) 15 pp. 7644-7644 [ Pubmed: 39223191 DOI: doi:10.1038/s41467-024-52174-z ]
- Frizzled-4 (60 kDa, Protein from Homo sapiens)
- Segment polarity protein dishevelled homolog dvl-2 (79 kDa, Protein from Homo sapiens)
- Gpcr complex (450 kDa, Complex from Homo sapiens)
CryoET of VSV incubated with liposomes at pH 5.5
Milojevic L, Si Z, Xia X, Chen L, He Y, Tang S, Luo M, Zhou ZH
Sci Adv (2024) 10 pp. eadn8579-eadn8579 [ Pubmed: 39630917 DOI: doi:10.1126/sciadv.adn8579 ]
CryoET of VSV incubated with liposomes at pH 5.5
Milojevic L, Si Z, Xia X, Chen L, He Y, Tang S, Luo M, Zhou ZH
Sci Adv (2024) 10 pp. eadn8579-eadn8579 [ Pubmed: 39630917 DOI: doi:10.1126/sciadv.adn8579 ]
CryoET of VSV incubated with liposomes at pH 5.5
Milojevic L, Si Z, Xia X, Chen L, He Y, Tang S, Luo M, Zhou ZH
Sci Adv (2024) 10 pp. eadn8579-eadn8579 [ Pubmed: 39630917 DOI: doi:10.1126/sciadv.adn8579 ]
CryoET of VSV incubated with liposomes at pH 5.5
Milojevic L, Si Z, Xia X, Chen L, He Y, Tang S, Luo M, Zhou ZH
Sci Adv (2024) 10 pp. eadn8579-eadn8579 [ Pubmed: 39630917 DOI: doi:10.1126/sciadv.adn8579 ]