GCGR-Gs complex in the presence of RAMP2
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8fu6
Q-score: 0.543
Krishna Kumar K, O'Brien ES, Habrian CH, Latorraca NR, Wang H, Tuneew I, Montabana E, Marqusee S, Hilger D, Isacoff EY, Mathiesen JM, Kobilka BK
Cell (2023) 186 pp. 1465-1477.e18 [ Pubmed: 37001505 DOI: doi:10.1016/j.cell.2023.02.028 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nb35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gcgr-gs signaling complex in the presence of ramp2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Glucagon derivative zp3780 (3 kDa, Protein from synthetic construct)
- Glucagon receptor (56 kDa, Protein from Homo sapiens)
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 7piu
Q-score: 0.6
Heyder NA, Kleinau G, Speck D, Schmidt A, Paisdzior S, Szczepek M, Bauer B, Koch A, Gallandi M, Kwiatkowski D, Burger J, Mielke T, Beck-Sickinger AG, Hildebrand PW, Spahn CMT, Hilger D, Schacherl M, Biebermann H, Hilal T, Kuhnen P, Kobilka BK, Scheerer P
Cell Res (2021) 31 pp. 1176-1189 [ DOI: doi:10.1038/s41422-021-00569-8 Pubmed: 34561620 ]
- Ternary complex of setmelanotide-activated melanocortin 4 receptor with heterotrimeric gs, further stabilized by addition of nanobody 35 (144 MDa, Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Setmelanotide (other names rm-493; bim-22493; irc-022493; imcivree) (1 kDa, Protein from synthetic construct)
- Camelid antibody fragment - nanobody 35 (14 kDa, Protein from Lama glama)
- Calcium ion (40 Da, Ligand)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Melanocortin receptor 4 (38 kDa, Protein from Homo sapiens)
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 7piv
Q-score: 0.539
Heyder NA, Kleinau G, Speck D, Schmidt A, Paisdzior S, Szczepek M, Bauer B, Koch A, Gallandi M, Kwiatkowski D, Burger J, Mielke T, Beck-Sickinger AG, Hildebrand PW, Spahn CMT, Hilger D, Schacherl M, Biebermann H, Hilal T, Kuhnen P, Kobilka BK, Scheerer P
Cell Res (2021) 31 pp. 1176-1189 [ DOI: doi:10.1038/s41422-021-00569-8 Pubmed: 34561620 ]
- Ternary complex of the ndp-alpha-msh activated melanocortin 4 receptor (mc4r) gs protein complex stabilized by nanobody 35 (144 MDa, Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Ndp-alpha-msh (other names afamelanotide; scenesse) (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Mus musculus)
- Calcium ion (40 Da, Ligand)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Camelid antibody vhh fragment - nanobody 35 (14 kDa, Protein from Lama glama)
- Melanocortin receptor 4 (38 kDa, Protein from Homo sapiens)
GCGR-Gs signaling complex bound to a designed glucagon derivative
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6wpw
Q-score: 0.518
Hilger D, Kumar KK, Hu H, Pedersen MF, O'Brien ES, Giehm L, Jennings C, Eskici G, Inoue A, Lerch M, Mathiesen JM, Skiniotis G, Kobilka BK
Science (2020) 369 [ DOI: doi:10.1126/science.aba3373 Pubmed: 32732395 ]
- Designed glucagon derivative (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nb35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gcgr (Complex from Homo sapiens)
- Nb35 (Complex from Lama glama)
- Gcgr-gs signaling complex bound to a designed glucagon derivative (163 kDa, Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Glucagon derivative zp3780 (3 kDa, Protein from synthetic construct)
- Gs (Complex from Homo sapiens)
- Glucagon receptor (56 kDa, Protein from Homo sapiens)
Structure of G-alpha-q bound to its chaperone Ric-8A
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6vu5
Q-score: 0.454
Seven AB, Hilger D, Papasergi-Scott MM, Zhang L, Qu Q, Kobilka BK, Tall GG, Skiniotis G
Cell Rep (2020) 30 pp. 3699-3709.e6 [ DOI: doi:10.1016/j.celrep.2020.02.086 Pubmed: 32126208 ]
Structure of G-alpha-i bound to its chaperone Ric-8A
Resolution: 4.14 Å
EM Method: Single-particle
Fitted PDBs: 6vu8
Q-score: 0.379
Seven AB, Hilger D, Papasergi-Scott MM, Zhang L, Qu Q, Kobilka BK, Tall GG, Skiniotis G
Cell Rep (2020) 30 pp. 3699-3709.e6 [ DOI: doi:10.1016/j.celrep.2020.02.086 Pubmed: 32126208 ]
human Neurotensin Receptor 1 (hNTSR1) - Gi1 Protein Complex in canonical conformation (C state)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6os9
Q-score: 0.51
Kato HE, Zhang Y, Hu H, Suomivuori CM, Kadji FMN, Aoki J, Krishna Kumar K, Fonseca R, Hilger D, Huang W, Latorraca NR, Inoue A, Dror RO, Kobilka BK, Skiniotis G
Nature (2019) 572 pp. 80-85 [ DOI: doi:10.1038/s41586-019-1337-6 Pubmed: 31243364 ]
- Neurotensin receptor type 1 (48 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Jmv449 (762 Da, Protein from synthetic construct)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Hntsr1-gi1 complex in canonical conformation (c state) (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
human Neurotensin Receptor 1 (hNTSR1) - Gi1 Protein Complex in non-canonical conformation (NC state)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6osa
Q-score: 0.497
Kato HE, Zhang Y, Hu H, Suomivuori CM, Kadji FMN, Aoki J, Krishna Kumar K, Fonseca R, Hilger D, Huang W, Latorraca NR, Inoue A, Dror RO, Kobilka BK, Skiniotis G
Nature (2019) 572 pp. 80-85 [ DOI: doi:10.1038/s41586-019-1337-6 Pubmed: 31243364 ]
- Neurotensin receptor type 1 (48 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Jmv449 (762 Da, Protein from synthetic construct)
- Hntsr1-gi1 complex in non-canonical conformation (nc state) (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Cannabinoid Receptor 1-G Protein Complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6n4b
Q-score: 0.506
Krishna Kumar K, Shalev-Benami M, Robertson MJ, Hu H, Banister SD, Hollingsworth SA, Latorraca NR, Kato HE, Hilger D, Maeda S, Weis WI, Farrens DL, Dror RO, Malhotra SV, Kobilka BK, Skiniotis G
Cell (2019) 176 pp. 448- [ Pubmed: 30639101 DOI: doi:10.1016/j.cell.2018.11.040 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Cannabinoid receptor 1-g protein complex (Complex from Homo sapiens)
- Cannabinoid receptor 1 (55 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Methyl n-{1-[(4-fluorophenyl)methyl]-1h-indazole-3-carbonyl}-3-methyl-l-valinate (397 Da, Ligand)
Mu Opioid Receptor-Gi Protein Complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6dde
Q-score: 0.407
Koehl A, Hu H, Maeda S, Zhang Y, Qu Q, Paggi JM, Latorraca NR, Hilger D, Dawson R, Matile H, Schertler GFX, Granier S, Weis WI, Dror RO, Manglik A, Skiniotis G, Kobilka BK
Nature (2018) 558 pp. 547-552 [ DOI: doi:10.1038/s41586-018-0219-7 Pubmed: 29899455 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Damgo (513 Da, Protein from Homo sapiens)
- Ternary complex of damgo-activated mu-type opioid receptor with heterotrimeric gi, further stabilized by addition of scfv-16 (130 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mu-type opioid receptor (39 kDa, Protein from Mus musculus)