Cryo-EM structure of the AsCas12f-sgRNA-target DNA ternary complex
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8j12
Q-score: 0.479
Hino T, Omura SN, Nakagawa R, Togashi T, Takeda SN, Hiramoto T, Tasaka S, Hirano H, Tokuyama T, Uosaki H, Ishiguro S, Kagieva M, Yamano H, Ozaki Y, Motooka D, Mori H, Kirita Y, Kise Y, Itoh Y, Matoba S, Aburatani H, Yachie N, Karvelis T, Siksnys V, Ohmori T, Hoshino A, Nureki O
Cell (2023) 186 pp. 4920-4935.e23 [ DOI: doi:10.1016/j.cell.2023.08.031 Pubmed: 37776859 ]
- Zinc ion (65 Da, Ligand)
- Rna (247-mer) (72 kDa, RNA from Acidibacillus sulfooxidans)
- Dna (38-mer) (11 kDa, DNA from Acidibacillus sulfooxidans)
- Dna (38-mer) (11 kDa, DNA from Acidibacillus sulfooxidans)
- Ascas12f-sgrna-target dna (Complex from Mycolicibacterium mucogenicum)
- Magnesium ion (24 Da, Ligand)
- Transposase is605 orfb c-terminal domain-containing protein (49 kDa, Protein from Acidibacillus sulfuroxidans)
Cryo-EM structure of the AsCas12f-YHAM-sgRNAS3-5v7-target DNA
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8j1j
Q-score: 0.511
Hino T, Omura SN, Nakagawa R, Togashi T, Takeda SN, Hiramoto T, Tasaka S, Hirano H, Tokuyama T, Uosaki H, Ishiguro S, Kagieva M, Yamano H, Ozaki Y, Motooka D, Mori H, Kirita Y, Kise Y, Itoh Y, Matoba S, Aburatani H, Yachie N, Karvelis T, Siksnys V, Ohmori T, Hoshino A, Nureki O
Cell (2023) 186 pp. 4920-4935.e23 [ DOI: doi:10.1016/j.cell.2023.08.031 Pubmed: 37776859 ]
- Zinc ion (65 Da, Ligand)
- Dna (38-mer) (11 kDa, DNA from Sulfoacidibacillus thermotolerans)
- Ascas12f-yham-sgrnas3-5v7-target dna (Complex from Sulfoacidibacillus thermotolerans)
- Rna (118-mer) (38 kDa, RNA from Sulfoacidibacillus thermotolerans)
- Magnesium ion (24 Da, Ligand)
- Dna (38-mer) (11 kDa, DNA from Sulfoacidibacillus thermotolerans)
- Transposase is605 orfb c-terminal domain-containing protein (49 kDa, Protein from Sulfoacidibacillus thermotolerans)
Cryo-EM structure of the AsCas12f-HKRA-sgRNAS3-5v7-target DNA
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 8j3r
Q-score: 0.174
Hino T, Omura SN, Nakagawa R, Togashi T, Takeda SN, Hiramoto T, Tasaka S, Hirano H, Tokuyama T, Uosaki H, Ishiguro S, Kagieva M, Yamano H, Ozaki Y, Motooka D, Mori H, Kirita Y, Kise Y, Itoh Y, Matoba S, Aburatani H, Yachie N, Karvelis T, Siksnys V, Ohmori T, Hoshino A, Nureki O
Cell (2023) 186 pp. 4920-4935.e23 [ DOI: doi:10.1016/j.cell.2023.08.031 Pubmed: 37776859 ]
- Rna (Complex)
- Dna (37-mer) (11 kDa, DNA from Sulfoacidibacillus thermotolerans)
- Zinc ion (65 Da, Ligand)
- Rna (118-mer) (38 kDa, RNA from Sulfoacidibacillus thermotolerans)
- Ascas12f-hkra-sgrnas3-5v7-target dna (Complex from Sulfoacidibacillus thermotolerans)
- Dna (Complex from Sulfoacidibacillus thermotolerans)
- Transposase is605 orfb c-terminal domain-containing protein (49 kDa, Protein from Sulfoacidibacillus thermotolerans)
- Dna (38-mer) (11 kDa, DNA from Sulfoacidibacillus thermotolerans)
- Magnesium ion (24 Da, Ligand)
- Ascas12f-hkra (Complex from Sulfoacidibacillus thermotolerans)
Structural insights into the human Prostaglandin E receptor EP3-Gi signaling complex
Suno R, Sugita Y, Morimoto K, Takazaki H, Tsujimoto H, Hirose M, Suno-Ikeda C, Nomura N, Hino T, Inoue A, Iwasaki K, Kato T, Iwata S, Kobayashi T
Cell Rep (2022) 40 [ Pubmed: 36103815 DOI: 10.1016/j.celrep.2022.111323 ]
Image sets:
Cryo-EM structure of the human EP3-Gi signaling complex
Resolution: 3.375 Å
EM Method: Single-particle
Fitted PDBs: 7wu9
Q-score: 0.466
Suno R, Sugita Y, Morimoto K, Takazaki H, Tsujimoto H, Hirose M, Suno-Ikeda C, Nomura N, Hino T, Inoue A, Iwasaki K, Kato T, Iwata S, Kobayashi T
Cell Rep (2022) 40 pp. 111323-111323 [ DOI: doi:10.1016/j.celrep.2022.111323 Pubmed: 36103815 ]
- Prostaglandine e receptor ep3-gi complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Prostaglandine e receptor ep3-gi complex (Complex)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Prostaglandin e2 receptor ep3 subtype (36 kDa, Protein from Homo sapiens)
Cryo-EM structure of mouse TRPV3 in a nanodisc
Shimada H, Kusakizako T, Dung Nguyen TH, Nishizawa T, Hino T, Tominaga M, Nureki O
Nat Struct Mol Biol (2020) 27 pp. 645-652 [ DOI: 10.1038/s41594-020-0439-z Pubmed: 32572254 ]
Image sets:
cryo-EM structure of TRPV3 in lipid nanodisc
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6lgp
Q-score: 0.531
Shimada H, Kusakizako T, Dung Nguyen TH, Nishizawa T, Hino T, Tominaga M, Nureki O
Nat Struct Mol Biol (2020) 27 pp. 645-652 [ DOI: doi:10.1038/s41594-020-0439-z Pubmed: 32572254 ]
- Trpv3 (Cellular component from Mus musculus)
- Transient receptor potential cation channel subfamily v member 3 (73 kDa, Protein from Mus musculus)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
MicroED structure of a FIB-milled CypA Crystal
Resolution: 2.5 Å
EM Method: Electron Crystallography
Fitted PDBs: 6u5g
Q-score: 0.702
Wolff AM, Young ID, Sierra RG, Brewster AS, Martynowycz MW, Nango E, Sugahara M, Nakane T, Ito K, Aquila A, Bhowmick A, Biel JT, Carbajo S, Cohen AE, Cortez S, Gonzalez A, Hino T, Im D, Koralek JD, Kubo M, Lazarou TS, Nomura T, Owada S, Samelson A, Tanaka T, Tanaka R, Thompson EM, van den Bedem H, Woldeyes RA, Yumoto F, Zhao W, Tono K, Boutet S, Iwata S, Gonen T, Sauter NK, Fraser JS, Thompson MC
IUCrJ (2020) 7 pp. 306-323 [ DOI: doi:10.1107/S205225252000072X ]
- Peptidyl-prolyl cis-trans isomerase a (18 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase a (Complex from Homo sapiens)