CryoEM structure of Gq-coupled MRGPRX2 with peptide agonist Cortistatin-14
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 7s8l
Q-score: 0.602
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ Pubmed: 34789874 DOI: doi:10.1038/s41586-021-04126-6 ]
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mrgprx2-gq cortistatin-14 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x2 (37 kDa, Protein from Homo sapiens)
- Cortistatin 14 (1 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
CryoEM structure of Gi-coupled MRGPRX2 with peptide agonist Cortistatin-14
Resolution: 2.54 Å
EM Method: Single-particle
Fitted PDBs: 7s8m
Q-score: 0.562
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ Pubmed: 34789874 DOI: doi:10.1038/s41586-021-04126-6 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Mrgprx2-gi1 cortistatin-14 (150 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cortistatin 14 (1 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- Soluble cytochrome b562, mas-related g-protein coupled receptor member x2 chimera (54 kDa, Protein from Homo sapiens)
CryoEM structure of Gq-coupled MRGPRX2 with small molecule agonist (R)-Zinc-3573
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7s8n
Q-score: 0.579
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ Pubmed: 34789874 DOI: doi:10.1038/s41586-021-04126-6 ]
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member x2 (37 kDa, Protein from Homo sapiens)
- Mrgprx2-gq (r)-zinc-3573 (150 MDa, Complex from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- (3r)-n,n-dimethyl-1-[(8s)-5-phenylpyrazolo[1,5-a]pyrimidin-7-yl]pyrrolidin-3-amine (307 Da, Ligand)
CryoEM structure of Gi-coupled MRGPRX2 with small molecule agonist (R)-Zinc-3573
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 7s8o
Q-score: 0.591
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ Pubmed: 34789874 DOI: doi:10.1038/s41586-021-04126-6 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- Mrgprx2-gi1 (r)-zinc-3573 (150 MDa, Complex from Homo sapiens)
- Soluble cytochrome b562,mas-related g-protein coupled receptor member x2 chimera (54 kDa, Protein from Homo sapiens)
- (3r)-n,n-dimethyl-1-[(8s)-5-phenylpyrazolo[1,5-a]pyrimidin-7-yl]pyrrolidin-3-amine (307 Da, Ligand)
CryoEM structure of Gq-coupled MRGPRX4 with small molecule agonist MS47134
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7s8p
Q-score: 0.605
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ Pubmed: 34789874 DOI: doi:10.1038/s41586-021-04126-6 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Mrgprx4-gq ms47134 (Complex from Homo sapiens)
- N-[(1r,3r,5s,7r)-3,5-dimethyltricyclo[3.3.1.1~3,7~]decane-1-carbonyl]-d-phenylalanine (355 Da, Ligand)
- Soluble cytochrome b562,mas-related g-protein coupled receptor member x4 chimera (53 kDa, Protein from Homo sapiens)
Cryo-EM structure of TMEM16F in digitonin with calcium bound
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6p46
Q-score: 0.403
Feng S, Dang S, Han TW, Ye W, Jin P, Cheng T, Li J, Jan YN, Jan LY, Cheng Y
Cell Rep (2019) 28 pp. 567-579.e4 [ Pubmed: 31291589 DOI: doi:10.1016/j.celrep.2019.06.023 ]
- Anoctamin-6 (106 kDa, Protein from Mus musculus)
- Calcium ion (40 Da, Ligand)
- Tmem16f with calcium bound (Complex from Mus musculus)
Cryo-EM structure of TMEM16F in digitonin without calcium
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6p47
Q-score: 0.365
Feng S, Dang S, Han TW, Ye W, Jin P, Cheng T, Li J, Jan YN, Jan LY, Cheng Y
Cell Rep (2019) 28 pp. 567-579.e4 [ Pubmed: 31291589 DOI: doi:10.1016/j.celrep.2019.06.023 ]
- Anoctamin-6 (106 kDa, Protein from Mus musculus)
- Tmem16f without calcium bound (Complex from Mus musculus)
Cryo-EM structure of calcium-bound TMEM16F in nanodisc with supplement of PIP2 in Cl1
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6p48
Q-score: 0.383
Feng S, Dang S, Han TW, Ye W, Jin P, Cheng T, Li J, Jan YN, Jan LY, Cheng Y
Cell Rep (2019) 28 pp. 567-579.e4 [ Pubmed: 31291589 DOI: doi:10.1016/j.celrep.2019.06.023 ]
- Anoctamin-6 (106 kDa, Protein from Mus musculus)
- Calcium ion (40 Da, Ligand)
- Tmem16f with calcium bound (Complex from Mus musculus)
Cryo-EM structure of calcium-bound TMEM16F in nanodisc with supplement of PIP2 in Cl2
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6p49
Q-score: 0.338
Feng S, Dang S, Han TW, Ye W, Jin P, Cheng T, Li J, Jan YN, Jan LY, Cheng Y
Cell Rep (2019) 28 pp. 567-579.e4 [ Pubmed: 31291589 DOI: doi:10.1016/j.celrep.2019.06.023 ]
- Anoctamin-6 (106 kDa, Protein from Mus musculus)
- Calcium ion (40 Da, Ligand)
- Tmem16f with calcium bound (Complex from Mus musculus)
Structure of a mechanotransduction ion channel Drosophila NOMPC in nanodisc
Resolution: 3.55 Å
EM Method: Single-particle
Fitted PDBs: 5vkq
Q-score: 0.378
Jin P, Bulkley D, Guo Y, Zhang W, Guo Z, Huynh W, Wu S, Meltzer S, Cheng T, Jan LY, Jan YN, Cheng Y
Nature (2017) 547 pp. 118-122 [ Pubmed: 28658211 DOI: doi:10.1038/nature22981 ]
- No mechanoreceptor potential c isoform l (188 kDa, Protein from Drosophila melanogaster)
- Tetrameric channel (754 kDa, Complex from Drosophila melanogaster)
- 1,2-diacyl-sn-glycero-3-phoshocholine (734 Da, Ligand)