Inward-facing conformation of the ABC transporter BmrA C436S/A582C cross-linked mutant
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 8chb
Q-score: 0.437
Di Cesare M, Kaplan E, Rendon J, Gerbaud G, Valimehr S, Gobet A, Ngo TT, Chaptal V, Falson P, Martinho M, Dorlet P, Hanssen E, Jault JM, Orelle C
J Biol Chem (2023) 300 pp. 105546-105546 [ Pubmed: 38072053 DOI: doi:10.1016/j.jbc.2023.105546 ]
Inward-facing conformation of the ABC transporter BmrA
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 8qoe
Q-score: 0.459
Di Cesare M, Kaplan E, Rendon J, Gerbaud G, Valimehr S, Gobet A, Ngo TT, Chaptal V, Falson P, Martinho M, Dorlet P, Hanssen E, Jault JM, Orelle C
J Biol Chem (2023) 300 pp. 105546-105546 [ Pubmed: 38072053 DOI: doi:10.1016/j.jbc.2023.105546 ]
CryoEM structure of the ABC transporter BmrA E504A mutant in complex with ATP-Mg
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7ow8
Q-score: 0.437
Chaptal V, Zampieri V, Wiseman B, Orelle C, Martin J, Nguyen KA, Gobet A, Di Cesare M, Magnard S, Javed W, Eid J, Kilburg A, Peuchmaur M, Marcoux J, Monticelli L, Hogbom M, Schoehn G, Jault JM, Boumendjel A, Falson P
Sci Adv (2022) 8 pp. eabg9215-eabg9215 [ DOI: doi:10.1126/sciadv.abg9215 Pubmed: 35080979 ]
- Multidrug resistance abc transporter atp-binding/permease protein bmra (65 kDa, Protein from Bacillus subtilis (strain 168))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Complex of bmra with atp-mg (130 kDa, Complex from Bacillus subtilis)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7bg4
Q-score: 0.309
Chaptal V, Zampieri V, Wiseman B, Orelle C, Martin J, Nguyen KA, Gobet A, Di Cesare M, Magnard S, Javed W, Eid J, Kilburg A, Peuchmaur M, Marcoux J, Monticelli L, Hogbom M, Schoehn G, Jault JM, Boumendjel A, Falson P
Sci Adv (2022) 8 pp. eabg9215-eabg9215 [ DOI: doi:10.1126/sciadv.abg9215 Pubmed: 35080979 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Multidrug resistance abc transporter atp-binding/permease protein bmra (65 kDa, Protein from Bacillus subtilis)
- Rhodamine 6g (443 Da, Ligand)
- Multidrug resistance transporter from bacillus subtilis bound with atp, mg, and rhodamine 6g. (Complex from Bacillus subtilis)
Multidrug resistance transporter BmrA mutant E504A bound with ATP and Mg solved by Cryo-EM
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6r81
Q-score: 0.359
Chaptal V, Zampieri V, Wiseman B, Orelle C, Martin J, Nguyen KA, Gobet A, Di Cesare M, Magnard S, Javed W, Eid J, Kilburg A, Peuchmaur M, Marcoux J, Monticelli L, Hogbom M, Schoehn G, Jault JM, Boumendjel A, Falson P
Sci Adv (2022) 8 pp. eabg9215-eabg9215 [ DOI: doi:10.1126/sciadv.abg9215 Pubmed: 35080979 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Lipid a export atp-binding/permease protein msba (65 kDa, Protein from Bacillus subtilis)
- Multidrug resistance transporter from bacillus subtilis bound with atp and mg. (Complex from Bacillus subtilis)
- Magnesium ion (24 Da, Ligand)
Electron cryo-microscopy of ABC BmrA in 12-fold symmetry rings
Fribourg PF, Chami M, Sorzano CO, Gubellini F, Marabini R, Marco S, Jault JM, Levy D
J Mol Biol (2014) 426 pp. 2059-2069 [ Pubmed: 24630999 DOI: doi:10.1016/j.jmb.2014.03.002 ]
- Bmra reconstituted at high density into lipid bilayer. (3 MDa, Sample)
- Bmra (3 MDa, Protein from Bacillus subtilis)
Electron cryo-microscopy of ABC BmrA in 13-fold symmetry rings
Fribourg PF, Chami M, Sorzano CO, Gubellini F, Marabini R, Marco S, Jault JM, Levy D
J Mol Biol (2014) 426 pp. 2059-2069 [ Pubmed: 24630999 DOI: doi:10.1016/j.jmb.2014.03.002 ]
- Bmra reconstituted at high density into lipid bilayer. (5 MDa, Sample)
- Bmra (5 MDa, Protein from Bacillus subtilis)