CryoEM structure of AcrIIA2 in complex with CRISPR-Cas9
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6mcb
Q-score: 0.438
Jiang F, Liu JJ, Osuna BA, Xu M, Berry JD, Rauch BJ, Nogales E, Bondy-Denomy J, Doudna JA
Mol Cell (2019) 73 pp. 601- [ Pubmed: 30595438 DOI: doi:10.1016/j.molcel.2018.11.016 ]
- Crispr-associated endonuclease cas9 (158 kDa, Protein from Streptococcus pyogenes M1 GAS)
- Anti-crispr protein acriia2 (14 kDa, Protein from Listeria phage LP-101)
- Acriia2 (Complex from Listeria phage LP-101)
- Single guide rna (116-mer) (37 kDa, RNA from Streptococcus pyogenes M1 GAS)
- Acriia2 interacting with sgrna-bound streptococcus pyogenes cas9 (Complex)
- Sgrna-bound streptococcus pyogenes cas9 (Complex from Streptococcus pyogenes M1 GAS)
CryoEM structure of AcrIIA2 homolog in complex with CRISPR-Cas9
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6mcc
Q-score: 0.385
Jiang F, Liu JJ, Osuna BA, Xu M, Berry JD, Rauch BJ, Nogales E, Bondy-Denomy J, Doudna JA
Mol Cell (2019) 73 pp. 601- [ Pubmed: 30595438 DOI: doi:10.1016/j.molcel.2018.11.016 ]
- Crispr-associated endonuclease cas9 (158 kDa, Protein from Streptococcus pyogenes M1 GAS)
- Acriia2 homolog (acriia2b) (Complex from Listeria phage LP-101)
- Single guide rna (116-mer) (37 kDa, RNA from Streptococcus pyogenes M1 GAS)
- Acriia2 homolog (acriia2b) bound to sgrna-bound streptococcus pyogenes cas9 (Complex)
- Anti-crispr acriia2 homolog (15 kDa, Protein from Listeria phage LP-101)
- Sgrna-bound streptococcus pyogenes cas9 (Complex from Streptococcus pyogenes M1 GAS)
cryo-EM structure of the Cas9-sgRNA-AcrIIA4 anti-CRISPR complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5vzl
Q-score: 0.006
Shin J, Jiang F, Liu JJ, Bray NL, Rauch BJ, Baik SH, Nogales E, Bondy-Denomy J, Corn JE, Doudna JA
Sci Adv (2017) 3 pp. e1701620-e1701620 [ Pubmed: 28706995 DOI: doi:10.1126/sciadv.1701620 ]
- Unidentified phage (Complex)
- Crispr-associated endonuclease cas9 (158 kDa, Protein from Streptococcus pyogenes M1 GAS)
- Streptococcus pyogenes m1 gas (Complex from unidentified phage)
- Single guide rna (116-mer) (38 kDa, RNA from Streptococcus pyogenes M1 GAS)
- Phage anti-crispr acriia4 (10 kDa, Protein from unidentified phage)
- The complex of streptococcus progenies cas9 with sgrna and acriia4 (Complex from Streptococcus pyogenes M1 GAS)
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA
Science (2016) 351 pp. 867-871 [ DOI: doi:10.1126/science.aad8282 Pubmed: 26841432 ]
- Cas9 bound to single guide-rna (194 kDa, Sample)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)
- Single guide-rna (36 kDa, RNA from Streptococcus pyogenes)
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA
Science (2016) 351 pp. 867-871 [ DOI: doi:10.1126/science.aad8282 Pubmed: 26841432 ]
- Cas9 bound to single guide-rna and 40-bp target dna (218 kDa, Sample)
- Single guide-rna (36 kDa, RNA from Streptococcus pyogenes)
- Target 40-bp double stranded dna (24 kDa, DNA)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)