Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 7udt
Q-score: 0.431
Gong R, Jiang F, Moreland ZG, Reynolds MJ, de Los Reyes SE, Gurel P, Shams A, Heidings JB, Bowl MR, Bird JE, Alushin GM
Sci Adv (2022) 8 pp. eabl4733-eabl4733 [ Pubmed: 35857845 DOI: doi:10.1126/sciadv.abl4733 ]
- Magnesium ion (24 Da, Ligand)
- Isoform 3 of unconventional myosin-xv (Complex from Mus musculus)
- Actin, alpha skeletal muscle (41 kDa, Protein from chicken)
- Regulatory light chain (16 kDa, Protein from Gallus gallus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin (Complex from Gallus gallus)
- Unconventional myosin-xv (82 kDa, Protein from Mus musculus)
- The rigor state wild type myosin-15-f-actin complex (5 MDa, Complex)
- Chicken muscle regulatory light chain (Complex from Gallus gallus)
Resolution: 4.15 Å
EM Method: Single-particle
Fitted PDBs: 7udu
Q-score: 0.341
Gong R, Jiang F, Moreland ZG, Reynolds MJ, de Los Reyes SE, Gurel P, Shams A, Heidings JB, Bowl MR, Bird JE, Alushin GM
Sci Adv (2022) 8 pp. eabl4733-eabl4733 [ Pubmed: 35857845 DOI: doi:10.1126/sciadv.abl4733 ]
- Magnesium ion (24 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from chicken)
- Isoform 3 of unconventional myosin-xv (Complex from Mus musculus)
- Regulatory light chain (16 kDa, Protein from Gallus gallus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin (Complex from Gallus gallus)
- Unconventional myosin-xv (82 kDa, Protein from Mus musculus)
- The rigor state wild type myosin-15-f-actin complex (5 MDa, Complex)
- Chicken muscle regulatory light chain (Complex from Gallus gallus)
Cryo-EM structure of the ADP state actin filament
Resolution: 2.82 Å
EM Method: Helical reconstruction
Fitted PDBs: 7r8v
Q-score: 0.612
Gong R, Jiang F, Moreland ZG, Reynolds MJ, de Los Reyes SE, Gurel P, Shams A, Heidings JB, Bowl MR, Bird JE, Alushin GM
Sci Adv (2022) 8 pp. eabl4733-eabl4733 [ Pubmed: 35857845 DOI: doi:10.1126/sciadv.abl4733 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Filamentous chicken skeletal muscle actin (5 MDa, Complex from Gallus gallus)
- Actin, alpha skeletal muscle, intermediate form (41 kDa, Protein from Chicken)
- Magnesium ion (24 Da, Ligand)
cryo-EM structure of the rigor state wild type myosin-15-F-actin complex
Resolution: 2.83 Å
EM Method: Helical reconstruction
Fitted PDBs: 7r91
Q-score: 0.547
Gong R, Jiang F, Moreland ZG, Reynolds MJ, de Los Reyes SE, Gurel P, Shams A, Heidings JB, Bowl MR, Bird JE, Alushin GM
Sci Adv (2022) 8 pp. eabl4733-eabl4733 [ Pubmed: 35857845 DOI: doi:10.1126/sciadv.abl4733 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Isoform 3 of unconventional myosin-xv (78 kDa, Protein from Mus musculus)
- Rigor state wild type myosin15 in complex with filamentous actin (5 MDa, Complex from Mus musculus)
- Actin, alpha skeletal muscle, intermediate form (41 kDa, Protein from Chicken)
cryo-EM structure of the ADP state wild type myosin-15-F-actin complex
Resolution: 3.63 Å
EM Method: Helical reconstruction
Fitted PDBs: 7rb8
Q-score: 0.442
Gong R, Jiang F, Moreland ZG, Reynolds MJ, de Los Reyes SE, Gurel P, Shams A, Heidings JB, Bowl MR, Bird JE, Alushin GM
Sci Adv (2022) 8 pp. eabl4733-eabl4733 [ Pubmed: 35857845 DOI: doi:10.1126/sciadv.abl4733 ]
- Magnesium ion (24 Da, Ligand)
- Isoform 3 of unconventional myosin-xv (Complex from Mus musculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin (Complex from Gallus gallus)
- The adp state wild type myosin15 in complex with filamentous actin (5 MDa, Complex)
- Isoform 3 of unconventional myosin-xv (78 kDa, Protein from Mus musculus)
- Actin, alpha skeletal muscle, intermediate form (41 kDa, Protein from Chicken)
Cryo-EM structure of the rigor state Jordan myosin-15-F-actin complex
Resolution: 3.76 Å
EM Method: Helical reconstruction
Fitted PDBs: 7rb9
Q-score: 0.407
Gong R, Jiang F, Moreland ZG, Reynolds MJ, de Los Reyes SE, Gurel P, Shams A, Heidings JB, Bowl MR, Bird JE, Alushin GM
Sci Adv (2022) 8 pp. eabl4733-eabl4733 [ Pubmed: 35857845 DOI: doi:10.1126/sciadv.abl4733 ]
- Magnesium ion (24 Da, Ligand)
- Isoform 3 of unconventional myosin-xv (Complex from Mus musculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin (Complex from Gallus gallus)
- The rigor state jordan myosin-15-f-actin complex (5 MDa, Complex)
- Isoform 3 of unconventional myosin-xv (78 kDa, Protein from Mus musculus)
- Actin, alpha skeletal muscle, intermediate form (41 kDa, Protein from Chicken)
cryo-EM structure of the Cas9-sgRNA-AcrIIA4 anti-CRISPR complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5vzl
Q-score: 0.006
Shin J, Jiang F, Liu JJ, Bray NL, Rauch BJ, Baik SH, Nogales E, Bondy-Denomy J, Corn JE, Doudna JA
Sci Adv (2017) 3 pp. e1701620-e1701620 [ Pubmed: 28706995 DOI: doi:10.1126/sciadv.1701620 ]
- Unidentified phage (Complex)
- Crispr-associated endonuclease cas9 (158 kDa, Protein from Streptococcus pyogenes M1 GAS)
- Streptococcus pyogenes m1 gas (Complex from unidentified phage)
- Single guide rna (116-mer) (38 kDa, RNA from Streptococcus pyogenes M1 GAS)
- Phage anti-crispr acriia4 (10 kDa, Protein from unidentified phage)
- The complex of streptococcus progenies cas9 with sgrna and acriia4 (Complex from Streptococcus pyogenes M1 GAS)
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA
Science (2016) 351 pp. 867-871 [ DOI: doi:10.1126/science.aad8282 Pubmed: 26841432 ]
- Cas9 bound to single guide-rna (194 kDa, Sample)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)
- Single guide-rna (36 kDa, RNA from Streptococcus pyogenes)
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA
Science (2016) 351 pp. 867-871 [ DOI: doi:10.1126/science.aad8282 Pubmed: 26841432 ]
- Cas9 bound to single guide-rna and 40-bp target dna (218 kDa, Sample)
- Single guide-rna (36 kDa, RNA from Streptococcus pyogenes)
- Target 40-bp double stranded dna (24 kDa, DNA)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)