Structure and dynamics of the essential endogenous mycobacterial polyketide synthase Pks13
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ Pubmed: 36782050 DOI: 10.1038/s41594-022-00918-0 ]
Image sets:
KS-AT di-domain of mycobacterial Pks13 with endogenous KS ligand bound
Resolution: 1.94 Å
EM Method: Single-particle
Fitted PDBs: 7uk4
Q-score: 0.628
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ DOI: doi:10.1038/s41594-022-00918-0 Pubmed: 36782050 ]
- Water (18 Da, Ligand)
- Unknown ligand (312 Da, Ligand)
- Ketosynthase domain (Complex from Mycolicibacterium smegmatis MC2 155)
- Acyl transferase domain (Complex from Mycolicibacterium smegmatis MC2 155)
- Polyketide synthase pks13 (198 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Mycobacterial polyketide synthase 13 (Complex from Mycolicibacterium smegmatis MC2 155)
ACP1-KS-AT domains of mycobacterial Pks13
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 8cuy
Q-score: 0.574
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ DOI: doi:10.1038/s41594-022-00918-0 Pubmed: 36782050 ]
KS-AT domains of mycobacterial Pks13 with inward AT conformation
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8cuz
Q-score: 0.522
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ DOI: doi:10.1038/s41594-022-00918-0 Pubmed: 36782050 ]
KS-AT domains of mycobacterial Pks13 with outward AT conformation
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8cv0
Q-score: 0.508
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ DOI: doi:10.1038/s41594-022-00918-0 Pubmed: 36782050 ]
ACP1-KS-AT domains of mycobacterial Pks13
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8cv1
Q-score: 0.543
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023) 30 pp. 296-308 [ DOI: doi:10.1038/s41594-022-00918-0 Pubmed: 36782050 ]
- Water (18 Da, Ligand)
- Unknown ligand (312 Da, Ligand)
- Polyketide synthase pks13 (194 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Mycobacterial polyketide synthase 13 (Complex from Mycolicibacterium smegmatis MC2 155)
Bartonella henselae NrnC bound to pGG. D4 Symmetry
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 7mqb
Q-score: 0.572
Lormand JD, Kim SK, Walters-Marrah GA, Brownfield BA, Fromme JC, Winkler WC, Goodson JR, Lee VT, Sondermann H
eLife (2021) 10 [ DOI: doi:10.7554/eLife.70146 Pubmed: 34533457 ]
- Rna (5'-r(p*gp*g)-3') (645 Da, RNA from synthetic construct)
- Nanornase c (23 kDa, Protein from Bartonella henselae)
- Pgg (Complex from Bartonella henselae)
- Nrnc octamer bound to pgg (186 MDa, Complex)
- Nrnc octamer (Complex from Bartonella henselae)
Bartonella henselae NrnC bound to pGG. C1 reconstruction.
Resolution: 3.64 Å
EM Method: Single-particle
Fitted PDBs: 7mqc
Q-score: 0.521
Lormand JD, Kim SK, Walters-Marrah GA, Brownfield BA, Fromme JC, Winkler WC, Goodson JR, Lee VT, Sondermann H
eLife (2021) 10 [ DOI: doi:10.7554/eLife.70146 Pubmed: 34533457 ]
- Rna (5'-r(p*gp*g)-3') (645 Da, RNA from synthetic construct)
- Nanornase c (23 kDa, Protein from Bartonella henselae)
- Pgg (Complex from Bartonella henselae)
- Nrnc octamer bound to pgg (186 MDa, Complex)
- Nrnc octamer (Complex from Bartonella henselae)
Bartonella henselae NrnC complexed with pAGG. D4 symmetry.
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 7mqd
Q-score: 0.566
Lormand JD, Kim SK, Walters-Marrah GA, Brownfield BA, Fromme JC, Winkler WC, Goodson JR, Lee VT, Sondermann H
eLife (2021) 10 [ DOI: doi:10.7554/eLife.70146 Pubmed: 34533457 ]
- Nrnc octamer incubated with pagg. (186 MDa, Complex)
- Pagg (Complex from Bartonella henselae)
- Rna (5'-r(p*ap*gp*g)-3') (974 Da, RNA from synthetic construct)
- Nanornase c (23 kDa, Protein from Bartonella henselae)
- Nrnc octamer (Complex from Bartonella henselae)
Bartonella henselae NrnC complexed with pAGG. C1 reconstruction.
Resolution: 3.69 Å
EM Method: Single-particle
Fitted PDBs: 7mqe
Q-score: 0.525
Lormand JD, Kim SK, Walters-Marrah GA, Brownfield BA, Fromme JC, Winkler WC, Goodson JR, Lee VT, Sondermann H
eLife (2021) 10 [ DOI: doi:10.7554/eLife.70146 Pubmed: 34533457 ]
- Nrnc octamer complexed with pagg. (186 MDa, Complex)
- Pagg (Complex from Bartonella henselae)
- Rna (5'-r(p*ap*gp*g)-3') (974 Da, RNA from synthetic construct)
- Nanornase c (23 kDa, Protein from Bartonella henselae)
- Nrnc octamer (Complex from Bartonella henselae)