De novo designed tetrahedral nanoparticle T33_dn10
Resolution: 3.86 Å
EM Method: Single-particle
Fitted PDBs: 6vfh
Q-score: 0.404
Ueda G, Antanasijevic A, Fallas JA, Sheffler W, Copps J, Ellis D, Hutchinson GB, Moyer A, Yasmeen A, Tsybovsky Y, Park YJ, Bick MJ, Sankaran B, Gillespie RA, Brouwer PJ, Zwart PH, Veesler D, Kanekiyo M, Graham BS, Sanders RW, Moore JP, Klasse PJ, Ward AB, King NP, Baker D
eLife (2020) 9 [ Pubmed: 32748788 DOI: doi:10.7554/eLife.57659 ]
- T33_dn10b (31 kDa, Protein from synthetic construct)
- T33_dn10a (14 kDa, Protein from synthetic construct)
- De novo designed tetrahedral nanoparticle t33_dn10 (546 kDa, Complex from synthetic construct)
De novo designed octahedral nanoparticle O43_dn18
Resolution: 4.54 Å
EM Method: Single-particle
Fitted PDBs: 6vfi
Q-score: 0.268
Ueda G, Antanasijevic A, Fallas JA, Sheffler W, Copps J, Ellis D, Hutchinson GB, Moyer A, Yasmeen A, Tsybovsky Y, Park YJ, Bick MJ, Sankaran B, Gillespie RA, Brouwer PJ, Zwart PH, Veesler D, Kanekiyo M, Graham BS, Sanders RW, Moore JP, Klasse PJ, Ward AB, King NP, Baker D
eLife (2020) 9 [ Pubmed: 32748788 DOI: doi:10.7554/eLife.57659 ]
- O43_dn18a (22 kDa, Protein from synthetic construct)
- De novo designed nanoparticle of octahedral symmetry o43_dn18_np (810 kDa, Complex from synthetic construct)
- O43_dn18b (13 kDa, Protein from synthetic construct)
De novo designed icosahedral nanoparticle I53_dn5
Resolution: 5.35 Å
EM Method: Single-particle
Fitted PDBs: 6vfj
Q-score: 0.166
Ueda G, Antanasijevic A, Fallas JA, Sheffler W, Copps J, Ellis D, Hutchinson GB, Moyer A, Yasmeen A, Tsybovsky Y, Park YJ, Bick MJ, Sankaran B, Gillespie RA, Brouwer PJ, Zwart PH, Veesler D, Kanekiyo M, Graham BS, Sanders RW, Moore JP, Klasse PJ, Ward AB, King NP, Baker D
eLife (2020) 9 [ Pubmed: 32748788 DOI: doi:10.7554/eLife.57659 ]
- I53_dn5a (17 kDa, Protein from synthetic construct)
- De novo designed nanoparticle of icosahedral symmetry i53_dn5_np (1 MDa, Complex from synthetic construct)
- I53_dn5b (15 kDa, Protein from synthetic construct)
Resolution: 4.25 Å
EM Method: Single-particle
Fitted PDBs: 6vfk
Q-score: 0.316
Antanasijevic A, Ueda G, Brouwer PJM, Copps J, Huang D, Allen JD, Cottrell CA, Yasmeen A, Sewall LM, Bontjer I, Ketas TJ, Turner HL, Berndsen ZT, Montefiori DC, Klasse PJ, Crispin M, Nemazee D, Moore JP, Sanders RW, King NP, Baker D, Ward AB
PLoS Pathog (2020) 16 pp. e1008665-e1008665 [ DOI: doi:10.1371/journal.ppat.1008665 Pubmed: 32780770 ]
- T33_dn10b (31 kDa, Protein from synthetic construct)
- De novo designed tetrahedral nanoparticle t33_dn10 displaying 4 copies of bg505-sosip trimer on the surface. nanoparticle consists of 12 copies of both subunits (bg505-sosip-t33_dn10a and t33_dn10b) (Complex from synthetic construct)
- Bg505-sosip-t33_dn10a (88 kDa, Protein from synthetic construct)
Resolution: 4.14 Å
EM Method: Single-particle
Fitted PDBs: 6vfl
Q-score: 0.404
Antanasijevic A, Ueda G, Brouwer PJM, Copps J, Huang D, Allen JD, Cottrell CA, Yasmeen A, Sewall LM, Bontjer I, Ketas TJ, Turner HL, Berndsen ZT, Montefiori DC, Klasse PJ, Crispin M, Nemazee D, Moore JP, Sanders RW, King NP, Baker D, Ward AB
PLoS Pathog (2020) 16 pp. e1008665-e1008665 [ DOI: doi:10.1371/journal.ppat.1008665 Pubmed: 32780770 ]
- Bg505-sosipv5.2(7s) - gp120 (56 kDa, Protein from synthetic construct)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Bg505-sosipv5.2(7s) - gp41 (17 kDa, Protein from synthetic construct)
- Bg505-sosip reconstructed from the tetrahedral nanoparticle t33_dn10 by subparticle extraction and refinement (Complex from synthetic construct)
Single Particle Electron Cryo-Microscopy Reconstruction of BG505 SOSIP-I53-50NP
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6p6f
Q-score: 0.301
Brouwer PJM, Antanasijevic A, Berndsen Z, Yasmeen A, Fiala B, Bijl TPL, Bontjer I, Bale JB, Sheffler W, Allen JD, Schorcht A, Burger JA, Camacho M, Ellis D, Cottrell CA, Behrens AJ, Catalano M, Del Moral-Sanchez I, Ketas TJ, LaBranche C, van Gils MJ, Sliepen K, Stewart LJ, Crispin M, Montefiori DC, Baker D, Moore JP, Klasse PJ, Ward AB, King NP, Sanders RW
Nat Commun (2019) 10 pp. 4272-4272 [ DOI: doi:10.1038/s41467-019-12080-1 Pubmed: 31537780 ]
- I53-50b.4pt1 (18 kDa, Protein from synthetic construct)
- I53-50b.4pt1 (Complex from synthetic construct)
- Two-component self-assembling nanoparticle consisting of bg505 sosip-i53-50a.1nt1 and i53-50b.4pt1 (Complex)
- I53-50a.1nt1 (99 kDa, Protein from synthetic construct)
- I53-50a.1nt1 (Complex from synthetic construct)