Klebl DP, McMillan SN, Risi C, Forgacs E, Virok B, Atherton JL, Stofella M, Winkelmann DA, Sobott F, Galkin VE, Knight PJ, Muench SP, Scarff CA, White HD
To Be Published
CryoEM structure of the primed actomyosin-5a complex
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 8r9v
Q-score: 0.148
Klebl DP, McMillan SN, Risi C, Forgacs E, Virok B, Atherton JL, Stofella M, Winkelmann DA, Sobott F, Galkin VE, Knight PJ, Muench SP, Scarff CA, White HD
To Be Published
- Phosphate ion (94 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Unconventional myosin-va (92 kDa, Protein from Mus musculus)
- Unconventional myosin-5a (Complex from Mus musculus)
- Primed actomyosin-5a complex (Complex from Mus musculus)
- F-actin (Complex from Oryctolagus cuniculus)
CryoEM structure of the post-powerstroke actomyosin-5a complex
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 8rbf
Q-score: 0.216
Klebl DP, McMillan SN, Risi C, Forgacs E, Virok B, Atherton JL, Stofella M, Winkelmann DA, Sobott F, Galkin VE, Knight PJ, Muench SP, Scarff CA, White HD
To Be Published
- Magnesium ion (24 Da, Ligand)
- Unconventional myosin-va (87 kDa, Protein from Mus musculus)
- Post-powerstroke actomyosin-5a complex (Complex from Mus musculus)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Unconventional myosin-5a (Complex from Oryctolagus cuniculus)
- F-actin (Complex)
CryoEM structure of primed myosin-5a (ADP-Pi state)
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 8rbg
Q-score: 0.208
Klebl DP, McMillan SN, Risi C, Forgacs E, Virok B, Atherton JL, Stofella M, Winkelmann DA, Sobott F, Galkin VE, Knight PJ, Muench SP, Scarff CA, White HD
To Be Published
- Phosphate ion (94 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Unconventional myosin-va (92 kDa, Protein from Mus musculus)
- Unconventional myosin-5a (Complex from Mus musculus)
Cryo-EM structure of actomyosin-5a-S1 with the full-length lever (nucleotide free, class D)
Resolution: 7.5 Å
EM Method: Single-particle
Fitted PDBs: 8of8
Q-score: 0.112
Gravett MSC, Klebl DP, Harlen OG, Read DJ, Harris SA, Muench SP, Peckham M
To Be Published
- Actin, alpha skeletal muscle (Complex from Oryctolagus cuniculus)
- Unconventional myosin-va with calmodulin-1 bound (Complex from Mus musculus)
- Unconventional myosin-va (106 kDa, Protein from Mus musculus)
- Myosin-5a bound to f-actin (20 kDa, Complex)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Calmodulin-1 (16 kDa, Protein from Mus musculus)
- Calmodulin-1 (Complex from Mus musculus)
Structure of the endocytic adaptor complex AENTH
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7b2l
Q-score: 0.373
Lizarrondo J, Klebl DP, Niebling S, Abella M, Schroer MA, Mertens HDT, Veith K, Thuenauer R, Svergun DI, Skruzny M, Sobott F, Muench SP, Garcia-Alai MM
Nat Commun (2021) 12 pp. 2889-2889 [ DOI: doi:10.1038/s41467-021-23151-7 Pubmed: 34001871 ]
- Enth domain of epsin ent1 (18 kDa, Protein from Saccharomyces cerevisiae S288C)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- 16 mer of 8 sla2 anth and 8 epsin-1 enth domains in complex with pip2 (Complex from Saccharomyces cerevisiae S288C)
- Anth domain of sla2 (33 kDa, Protein from Saccharomyces cerevisiae S288C)
Structure of the human mitochondrial HSPD1 single ring
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7azp
Q-score: 0.407
Klebl DP, Feasey MC, Hesketh EL, Ranson NA, Wurdak H, Sobott F, Bon RS, Muench SP
iScience (2021) 24 pp. 102022-102022 [ DOI: doi:10.1016/j.isci.2020.102022 Pubmed: 33506187 ]
HSPD1 single ring deposited by spraying (6 ms delay)
Klebl DP, Gravett MSC, Kontziampasis D, Wright DJ, Bon RS, Monteiro DCF, Trebbin M, Sobott F, White HD, Darrow MC, Thompson RF, Muench SP
Structure (2020) 28 pp. 1238-1248.e4 [ Pubmed: 32814033 DOI: doi:10.1016/j.str.2020.07.018 ]
HSPD1 single ring deposited by spraying (50 ms delay)
Klebl DP, Gravett MSC, Kontziampasis D, Wright DJ, Bon RS, Monteiro DCF, Trebbin M, Sobott F, White HD, Darrow MC, Thompson RF, Muench SP
Structure (2020) 28 pp. 1238-1248.e4 [ Pubmed: 32814033 DOI: doi:10.1016/j.str.2020.07.018 ]
HSPD1 single ring deposited using the chameleon (54 ms delay)
Klebl DP, Gravett MSC, Kontziampasis D, Wright DJ, Bon RS, Monteiro DCF, Trebbin M, Sobott F, White HD, Darrow MC, Thompson RF, Muench SP
Structure (2020) 28 pp. 1238-1248.e4 [ Pubmed: 32814033 DOI: doi:10.1016/j.str.2020.07.018 ]