Cryo-EM structure of E. coli FimH lectin domain bound to Fabs 440-2 and 454-3
Silmon de Monerri NC, Che Y, Lees JA, Jasti J, Wu H, Griffor MC, Kodali S, Hawkins JC, Lypowy J, Ponce C, Curley K, Esadze A, Carcamo J, McLellan T, Keeney D, Illenberger A, Matsuka YV, Shanker S, Chorro L, Gribenko AV, Han S, Anderson AS, Donald RGK
PLoS Pathog (2025) 21 pp. e1012325-e1012325 [ Pubmed: 39970181 DOI: doi:10.1371/journal.ppat.1012325 ]
- 440-2 fab light chain (24 kDa, Protein from Mus musculus)
- 440-2 fab heavy chain (24 kDa, Protein from Mus musculus)
- 445-3 fab heavy chain (24 kDa, Protein from Mus musculus)
- 445-3 fab light chain (23 kDa, Protein from Mus musculus)
- Type 1 fimbrin d-mannose specific adhesin fimh, donor strand complemented with fimg peptide 'triple mutant' (32 kDa, Protein from Escherichia coli)
- Fimh-dsg tm in complex with fabs 440-2 and 445-3 (Complex)
- Fimh-dsg tm (Complex from Escherichia coli)
- Fab 445-3 (Complex from Mus musculus)
- Fab 440-2 (Complex from Mus musculus)
Cryo-EM structure of E. coli FimH lectin domain bound to Fabs 329-2 and 454-3
Silmon de Monerri NC, Che Y, Lees JA, Jasti J, Wu H, Griffor MC, Kodali S, Hawkins JC, Lypowy J, Ponce C, Curley K, Esadze A, Carcamo J, McLellan T, Keeney D, Illenberger A, Matsuka YV, Shanker S, Chorro L, Gribenko AV, Han S, Anderson AS, Donald RGK
PLoS Pathog (2025) 21 pp. e1012325-e1012325 [ Pubmed: 39970181 DOI: doi:10.1371/journal.ppat.1012325 ]
- Fab 329-2 light chain (23 kDa, Protein from Mus musculus)
- Ternary complex of e. coli fimh with fabs 329-2 and 454-3 (Complex from Escherichia coli)
- Type 1 fimbrin d-mannose specific adhesin fimh, donor strand complemented with fimg peptide 'triple mutant' (32 kDa, Protein from Escherichia coli)
- Fab 329-2 heavy chain (24 kDa, Protein from Mus musculus)
- Fab 454-3 light chain (23 kDa, Protein from Mus musculus)
- Fab 454-3 heavy chain (23 kDa, Protein from Mus musculus)
Cryo-EM structure of Human respiratory syncytial virus F variant (construct pXCS847A)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7uja
Q-score: 0.492
Che Y, Gribenko AV, Song X, Handke LD, Efferen KS, Tompkins K, Kodali S, Nunez L, Prasad AK, Phelan LM, Ammirati M, Yu X, Lees JA, Chen W, Martinez L, Roopchand V, Han S, Qiu X, DeVincenzo JP, Jansen KU, Dormitzer PR, Swanson KA
Sci Transl Med (2023) 15 pp. eade6422-eade6422 [ Pubmed: 37023209 DOI: doi:10.1126/scitranslmed.ade6422 ]
- Am14 fab heavy chain (26 kDa, Protein from Mus musculus)
- Human respiratory syncytial virus f variant (construct pxcs847a) with fabs am14 and am22 (Complex from Respiratory syncytial virus)
- Rsv variant (construct pxcs847a) f2 (12 kDa, Protein from Respiratory syncytial virus)
- Am22 fab light chain (23 kDa, Protein from Mus musculus)
- Am22 fab heavy chain (25 kDa, Protein from Mus musculus)
- Rsv variant (construct pxcs847a) f1 (45 kDa, Protein from Respiratory syncytial virus)
- Am14 fab light chain (23 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)