The structure of the Tetrahymena thermophila outer dynein arm on doublet microtubule
Resolution: 8.0 Å
EM Method: Single-particle
Fitted PDBs: 7moq
Q-score: 0.067
Kubo S, Yang SK, Black CS, Dai D, Valente-Paterno M, Gaertig J, Ichikawa M, Bui KH
EMBO Rep (2021) 22 pp. e52911-e52911 [ DOI: doi:10.15252/embr.202152911 Pubmed: 34338432 ]
- Docking complex 1 protein (64 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein-1-alpha heavy chain, flagellar inner arm i1 complex protein, putative (475 kDa, Protein from Tetrahymena thermophila CU428)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (13 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm complex from tetrahymena thermophila (2 MDa, Cellular component from Tetrahymena thermophila CU428)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila CU428)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain roadblock-type 2 protein (18 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm docking complex protein oda protein (35 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm docking complex protein oda protein (63 kDa, Protein from Tetrahymena thermophila CU428)
- Outer arm dynein beta heavy chain (530 kDa, Protein from Tetrahymena thermophila CU428)
- Docking complex 1/2 protein (11 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Thioredoxin (12 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein intermediate chain 2 (77 kDa, Protein from Tetrahymena thermophila CU428)
Complex of yeast cytoplasmic dynein MTBD-High and MT without DTT
Resolution: 3.94 Å
EM Method: Helical reconstruction
Fitted PDBs: 6kio
Q-score: 0.414
Nishida N, Komori Y, Takarada O, Watanabe A, Tamura S, Kubo S, Shimada I, Kikkawa M
Nat Commun (2020) 11 pp. 1038-1038 [ DOI: doi:10.1038/s41467-020-14842-8 Pubmed: 32098965 ]
- Dynein heavy chain, cytoplasmic (15 kDa, Protein from Saccharomyces cerevisiae S288c)
- Tubulin alpha-1a chain (45 kDa, Protein from Sus scrofa)
- Mtbd-high/mt complex without dtt (Cell from Sus scrofa)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
Complex of yeast cytoplasmic dynein MTBD-High and MT with DTT
Resolution: 3.62 Å
EM Method: Helical reconstruction
Fitted PDBs: 6kiq
Q-score: 0.455
Nishida N, Komori Y, Takarada O, Watanabe A, Tamura S, Kubo S, Shimada I, Kikkawa M
Nat Commun (2020) 11 pp. 1038-1038 [ DOI: doi:10.1038/s41467-020-14842-8 Pubmed: 32098965 ]
- Alpha tubulin (45 kDa, Protein from Sus scrofa)
- Dynein heavy chain, cytoplasmic (15 kDa, Protein from Saccharomyces cerevisiae S288c)
- Mtbd-high/mt complex with dtt (Cell from Sus scrofa)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
The inner junction complex of Chlamydomonas reinhardtii doublet microtubule
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6ve7
Q-score: 0.455
Khalifa AAZ, Ichikawa M, Dai D, Kubo S, Black C, Peri K, McAlear TS, Veyron S, Yang SK, Vargas J, Bechstedt S, Trempe JF, Bui KH
eLife (2020) 9 [ DOI: doi:10.7554/eLife.52760 Pubmed: 31951202 ]
- Tubulin beta (49 kDa, Protein from Chlamydomonas reinhardtii)
- Cilia- and flagella-associated protein 20 (22 kDa, Protein from Chlamydomonas reinhardtii)
- Inner junction complex of the cilia (1 MDa, Cellular component from Chlamydomonas reinhardtii)
- Fap52 (68 kDa, Protein from Chlamydomonas reinhardtii)
- Pacrg (34 kDa, Protein from Chlamydomonas reinhardtii)
- Fap276 (10 kDa, Protein from Chlamydomonas reinhardtii)
- Protein flattop homolog (15 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar associated protein (27 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin alpha (49 kDa, Protein from Chlamydomonas reinhardtii)
Cryo-EM structure of the 48-nm repeat unit of the doublet microtubule from Tetrahymena thermophila
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6u0h
Q-score: 0.357
Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF, Vargas J, Bui KH
PNAS (2019) 116 pp. 19930-19938 [ DOI: doi:10.1073/pnas.1911119116 Pubmed: 31527277 ]
- 48nm-repeat of microtubule doublet (Cellular component from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila)
- Tubulin lattice (Complex from Tetrahymena thermophila)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila)
- Guanosine-5'-triphosphate (523 Da, Ligand)