CryoEM Structure INO80core Hexasome complex Rvb core refinement state1
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8ooc
Q-score: 0.568
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ DOI: doi:10.1126/science.adf6287 Pubmed: 37384673 ]
- Dash complex subunit dad4 (87 kDa, Protein from Thermochaetoides thermophila)
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Ruvb-like helicase (53 kDa, Protein from Thermochaetoides thermophila)
- Vps72/yl1 c-terminal domain-containing protein (23 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ino80 complex subunit b-like conserved region domain-containing protein (53 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Ruvb-like helicase (50 kDa, Protein from Thermochaetoides thermophila)
CryoEM Structure INO80core Hexasome complex composite map state1
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8oo7
Q-score: 0.556
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ DOI: doi:10.1126/science.adf6287 Pubmed: 37384673 ]
- Chromatin remodeler ino80 (Complex from Thermochaetoides thermophila)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Ino80 core module in complex with hexasome (861 kDa, Complex)
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Ruvb-like protein 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Dna (Complex from Synthetic construct)
- Ino eighty subunit 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Histones (Complex from Homo sapiens)
- Ruvb-like protein 1 (50 kDa, Protein from Thermochaetoides thermophila)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
CryoEM Structure INO80core Hexasome complex ATPase-DNA refinement state1
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8oo9
Q-score: 0.515
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ DOI: doi:10.1126/science.adf6287 Pubmed: 37384673 ]
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Magnesium ion (24 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
CryoEM Structure INO80core Hexasome complex Hexasome refinement state1
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8ooa
Q-score: 0.525
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ DOI: doi:10.1126/science.adf6287 Pubmed: 37384673 ]
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
CryoEM Structure INO80core Hexasome complex Arp5 Ies6 refinement state1
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8oof
Q-score: 0.571
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ DOI: doi:10.1126/science.adf6287 Pubmed: 37384673 ]
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
CryoEM Structure INO80core Hexasome complex Arp5 grappler refinement state1
Resolution: 5.69 Å
EM Method: Single-particle
Fitted PDBs: 8ook
Q-score: 0.251
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ DOI: doi:10.1126/science.adf6287 Pubmed: 37384673 ]
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
CryoEM Structure INO80core Hexasome complex overall refinement state1
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weiss F, diMaio F, Hopfner KP, Eustermann S
To Be Published
INO80 core bound to hexasome composite map of state 2
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8oop
Q-score: 0.554
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ DOI: doi:10.1126/science.adf6287 Pubmed: 37384673 ]
- Chromatin remodeler ino80 (Complex from Thermochaetoides thermophila)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Ino80 core module in complex with hexasome (861 kDa, Complex)
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Ruvb-like protein 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Dna (Complex from Synthetic construct)
- Ino eighty subunit 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Histones (Complex from Homo sapiens)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Ruvb-like protein 1 (50 kDa, Protein from Thermochaetoides thermophila)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
CryoEM Structure INO80core Hexasome complex Rvb core refinement state2
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8oor
Q-score: 0.597
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ DOI: doi:10.1126/science.adf6287 Pubmed: 37384673 ]
- Ruvb-like protein 1 (50 kDa, Protein from Thermochaetoides thermophila)
- Ruvb-like protein 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ino eighty subunit 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)