CryoEM Structure INO80core Hexasome complex Rvb core refinement state1
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8ooc
Q-score: 0.568
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ Pubmed: 37384673 DOI: doi:10.1126/science.adf6287 ]
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Ruvb-like helicase (53 kDa, Protein from Thermochaetoides thermophila)
- Dash complex subunit dad4 (87 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ino80 complex subunit b-like conserved region domain-containing protein (53 kDa, Protein from Thermochaetoides thermophila)
- Ruvb-like helicase (50 kDa, Protein from Thermochaetoides thermophila)
- Vps72/yl1 c-terminal domain-containing protein (23 kDa, Protein from Thermochaetoides thermophila)
CryoEM Structure INO80core Hexasome complex composite map state1
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8oo7
Q-score: 0.556
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ Pubmed: 37384673 DOI: doi:10.1126/science.adf6287 ]
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Histones (Complex from Homo sapiens)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ruvb-like protein 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Chromatin remodeler ino80 (Complex from Thermochaetoides thermophila)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Ruvb-like protein 1 (50 kDa, Protein from Thermochaetoides thermophila)
- Ino eighty subunit 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Dna (Complex from Synthetic construct)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Tetrafluoroaluminate ion (102 Da, Ligand)
CryoEM Structure INO80core Hexasome complex ATPase-DNA refinement state1
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8oo9
Q-score: 0.515
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ Pubmed: 37384673 DOI: doi:10.1126/science.adf6287 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Magnesium ion (24 Da, Ligand)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Tetrafluoroaluminate ion (102 Da, Ligand)
CryoEM Structure INO80core Hexasome complex Hexasome refinement state1
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8ooa
Q-score: 0.525
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ Pubmed: 37384673 DOI: doi:10.1126/science.adf6287 ]
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
CryoEM Structure INO80core Hexasome complex Arp5 Ies6 refinement state1
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8oof
Q-score: 0.571
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ Pubmed: 37384673 DOI: doi:10.1126/science.adf6287 ]
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
CryoEM Structure INO80core Hexasome complex Arp5 grappler refinement state1
Resolution: 5.69 Å
EM Method: Single-particle
Fitted PDBs: 8ook
Q-score: 0.251
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ Pubmed: 37384673 DOI: doi:10.1126/science.adf6287 ]
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
CryoEM Structure INO80core Hexasome complex overall refinement state1
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weiss F, diMaio F, Hopfner KP, Eustermann S
To Be Published
INO80 core bound to hexasome composite map of state 2
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8oop
Q-score: 0.554
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ Pubmed: 37384673 DOI: doi:10.1126/science.adf6287 ]
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Histones (Complex from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ruvb-like protein 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Chromatin remodeler ino80 (Complex from Thermochaetoides thermophila)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Ruvb-like protein 1 (50 kDa, Protein from Thermochaetoides thermophila)
- Ino eighty subunit 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Dna (Complex from Synthetic construct)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Tetrafluoroaluminate ion (102 Da, Ligand)
CryoEM Structure INO80core Hexasome complex Rvb core refinement state2
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8oor
Q-score: 0.597
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ Pubmed: 37384673 DOI: doi:10.1126/science.adf6287 ]
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ruvb-like protein 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Ruvb-like protein 1 (50 kDa, Protein from Thermochaetoides thermophila)
- Ino eighty subunit 2 (53 kDa, Protein from Thermochaetoides thermophila)