Structure of Arp4-Ies4-N-actin-Arp8-Ino80HSA subcomplex (A-module) of Chaetomium thermophilum INO80
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8a5d
Q-score: 0.572
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Ies4 (29 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 8 (84 kDa, Protein from Thermochaetoides thermophila)
- Actin related protein 4 (arp4) (51 kDa, Protein from Thermochaetoides thermophila)
- Ino80 atpase (37 kDa, Protein from Thermochaetoides thermophila)
- Actin (41 kDa, Protein from Thermochaetoides thermophila)
- Magnesium ion (24 Da, Ligand)
- Chaetomium thermophilum ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Thermochaetoides thermophila)
Cryo-EM reconstruction of Arp4-Ies4-N-actin-Arp8-Ino80HSA subcomplex (A-module) of INO80
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8a5a
Q-score: 0.509
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Saccharomyces cerevisiae S288C)
- Actin-like protein arp8 (100 kDa, Protein from Saccharomyces cerevisiae S288C)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Actin (41 kDa, Protein from Saccharomyces cerevisiae S288C)
- Chromatin-remodeling atpase ino80 (72 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ino eighty subunit 4 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- Actin-related protein 4 (54 kDa, Protein from Saccharomyces cerevisiae S288C)
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
- Ino80 (Protein from Saccharomyces cerevisiae S288C)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) adp state (Complex from Saccharomyces cerevisiae S288C)
- Ies4 (Protein from Saccharomyces cerevisiae S288C)
- Arp4 (Protein from Saccharomyces cerevisiae S288C)
- Actin (Protein from Saccharomyces cerevisiae S288C)
- Arp8 (Protein from Saccharomyces cerevisiae S288C)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8a5o
Q-score: 0.535
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Saccharomyces cerevisiae S288C)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Actin-like protein arp8 (100 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- Actin (41 kDa, Protein from Saccharomyces cerevisiae S288C)
- Chromatin-remodeling atpase ino80 (72 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ino eighty subunit 4 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- Actin-related protein 4 (54 kDa, Protein from Saccharomyces cerevisiae S288C)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8a5p
Q-score: 0.415
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
- Ies4 (29 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 8 (84 kDa, Protein from Thermochaetoides thermophila)
- Actin related protein 4 (arp4) (51 kDa, Protein from Thermochaetoides thermophila)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) on curved dna (Complex from Thermochaetoides thermophila)
- Dna (36-mer) (11 kDa, DNA from synthetic construct)
- Ino80 atpase (210 kDa, Protein from Thermochaetoides thermophila)
- Actin (41 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (36-mer) (11 kDa, DNA from synthetic construct)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8a5q
Q-score: 0.483
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
- Ies4 (29 kDa, Protein from Thermochaetoides thermophila)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Thermochaetoides thermophila)
- Actin-related protein 8 (84 kDa, Protein from Thermochaetoides thermophila)
- Actin related protein 4 (arp4) (51 kDa, Protein from Thermochaetoides thermophila)
- Dna (26-mer) (70 kDa, DNA from synthetic construct)
- Actin (41 kDa, Protein from Thermochaetoides thermophila)
- Ino80 atpase (210 kDa, Protein from Thermochaetoides thermophila)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) on straight dna (Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Dna (26-mer) (69 kDa, DNA from synthetic construct)
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
- Ino80 (Protein from Saccharomyces cerevisiae S288C)
- Ies4 (Protein from Saccharomyces cerevisiae S288C)
- Arp4 (Protein from Saccharomyces cerevisiae S288C)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) bound to dna (Complex from Saccharomyces cerevisiae S288C)
- Dna (DNA from Saccharomyces cerevisiae S288C)
- Actin (Protein from Saccharomyces cerevisiae S288C)
- Arp8 (Protein from Saccharomyces cerevisiae S288C)
A-module of Chaetomium thermophilum INO80 bound to curved DNA
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]