Structure of Arp4-Ies4-N-actin-Arp8-Ino80HSA subcomplex (A-module) of Chaetomium thermophilum INO80
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8a5d
Q-score: 0.572
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ Pubmed: 36490333 DOI: doi:10.1126/sciadv.add3189 ]
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Actin related protein 4 (arp4) (51 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 8 (84 kDa, Protein from Thermochaetoides thermophila)
- Actin (41 kDa, Protein from Thermochaetoides thermophila)
- Ino80 atpase (37 kDa, Protein from Thermochaetoides thermophila)
- Ies4 (29 kDa, Protein from Thermochaetoides thermophila)
- Chaetomium thermophilum ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Thermochaetoides thermophila)
Cryo-EM reconstruction of Arp4-Ies4-N-actin-Arp8-Ino80HSA subcomplex (A-module) of INO80
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8a5a
Q-score: 0.509
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ Pubmed: 36490333 DOI: doi:10.1126/sciadv.add3189 ]
- Ino eighty subunit 4 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin-related protein 4 (54 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Saccharomyces cerevisiae S288C)
- Actin-like protein arp8 (100 kDa, Protein from Saccharomyces cerevisiae S288C)
- Chromatin-remodeling atpase ino80 (72 kDa, Protein from Saccharomyces cerevisiae S288C)
- Actin (41 kDa, Protein from Saccharomyces cerevisiae S288C)
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ Pubmed: 36490333 DOI: doi:10.1126/sciadv.add3189 ]
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ Pubmed: 36490333 DOI: doi:10.1126/sciadv.add3189 ]
- Ies4 (Protein from Saccharomyces cerevisiae S288C)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) adp state (Complex from Saccharomyces cerevisiae S288C)
- Actin (Protein from Saccharomyces cerevisiae S288C)
- Arp4 (Protein from Saccharomyces cerevisiae S288C)
- Arp8 (Protein from Saccharomyces cerevisiae S288C)
- Ino80 (Protein from Saccharomyces cerevisiae S288C)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8a5o
Q-score: 0.535
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ Pubmed: 36490333 DOI: doi:10.1126/sciadv.add3189 ]
- Ino eighty subunit 4 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Actin-related protein 4 (54 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Saccharomyces cerevisiae S288C)
- Actin-like protein arp8 (100 kDa, Protein from Saccharomyces cerevisiae S288C)
- Chromatin-remodeling atpase ino80 (72 kDa, Protein from Saccharomyces cerevisiae S288C)
- Actin (41 kDa, Protein from Saccharomyces cerevisiae S288C)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8a5p
Q-score: 0.415
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ Pubmed: 36490333 DOI: doi:10.1126/sciadv.add3189 ]
- Dna (36-mer) (11 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin related protein 4 (arp4) (51 kDa, Protein from Thermochaetoides thermophila)
- Ino80 atpase (210 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 8 (84 kDa, Protein from Thermochaetoides thermophila)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) on curved dna (Complex from Thermochaetoides thermophila)
- Actin (41 kDa, Protein from Thermochaetoides thermophila)
- Ies4 (29 kDa, Protein from Thermochaetoides thermophila)
- Dna (36-mer) (11 kDa, DNA from synthetic construct)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8a5q
Q-score: 0.483
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ Pubmed: 36490333 DOI: doi:10.1126/sciadv.add3189 ]
- Dna (26-mer) (69 kDa, DNA from synthetic construct)
- Dna (26-mer) (70 kDa, DNA from synthetic construct)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin related protein 4 (arp4) (51 kDa, Protein from Thermochaetoides thermophila)
- Magnesium ion (24 Da, Ligand)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) on straight dna (Complex)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Thermochaetoides thermophila)
- Ino80 atpase (210 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 8 (84 kDa, Protein from Thermochaetoides thermophila)
- Actin (41 kDa, Protein from Thermochaetoides thermophila)
- Dna (Complex from synthetic construct)
- Ies4 (29 kDa, Protein from Thermochaetoides thermophila)
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ Pubmed: 36490333 DOI: doi:10.1126/sciadv.add3189 ]
- Dna (DNA from Saccharomyces cerevisiae S288C)
- Ies4 (Protein from Saccharomyces cerevisiae S288C)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) bound to dna (Complex from Saccharomyces cerevisiae S288C)
- Actin (Protein from Saccharomyces cerevisiae S288C)
- Arp4 (Protein from Saccharomyces cerevisiae S288C)
- Arp8 (Protein from Saccharomyces cerevisiae S288C)
- Ino80 (Protein from Saccharomyces cerevisiae S288C)
A-module of Chaetomium thermophilum INO80 bound to curved DNA
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ Pubmed: 36490333 DOI: doi:10.1126/sciadv.add3189 ]
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ Pubmed: 36490333 DOI: doi:10.1126/sciadv.add3189 ]