Structure of Arp4-Ies4-N-actin-Arp8-Ino80HSA subcomplex (A-module) of Chaetomium thermophilum INO80
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8a5d
Q-score: 0.572
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Ies4 (29 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 8 (84 kDa, Protein from Thermochaetoides thermophila)
- Actin related protein 4 (arp4) (51 kDa, Protein from Thermochaetoides thermophila)
- Ino80 atpase (37 kDa, Protein from Thermochaetoides thermophila)
- Actin (41 kDa, Protein from Thermochaetoides thermophila)
- Magnesium ion (24 Da, Ligand)
- Chaetomium thermophilum ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Thermochaetoides thermophila)
Cryo-EM reconstruction of Arp4-Ies4-N-actin-Arp8-Ino80HSA subcomplex (A-module) of INO80
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8a5a
Q-score: 0.509
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Saccharomyces cerevisiae S288C)
- Actin-like protein arp8 (100 kDa, Protein from Saccharomyces cerevisiae S288C)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Actin (41 kDa, Protein from Saccharomyces cerevisiae S288C)
- Chromatin-remodeling atpase ino80 (72 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ino eighty subunit 4 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- Actin-related protein 4 (54 kDa, Protein from Saccharomyces cerevisiae S288C)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8a5o
Q-score: 0.535
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Saccharomyces cerevisiae S288C)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Actin-like protein arp8 (100 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- Actin (41 kDa, Protein from Saccharomyces cerevisiae S288C)
- Chromatin-remodeling atpase ino80 (72 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ino eighty subunit 4 (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- Actin-related protein 4 (54 kDa, Protein from Saccharomyces cerevisiae S288C)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8a5p
Q-score: 0.415
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
- Ies4 (29 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 8 (84 kDa, Protein from Thermochaetoides thermophila)
- Actin related protein 4 (arp4) (51 kDa, Protein from Thermochaetoides thermophila)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) on curved dna (Complex from Thermochaetoides thermophila)
- Dna (36-mer) (11 kDa, DNA from synthetic construct)
- Ino80 atpase (210 kDa, Protein from Thermochaetoides thermophila)
- Actin (41 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (36-mer) (11 kDa, DNA from synthetic construct)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8a5q
Q-score: 0.483
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
- Ies4 (29 kDa, Protein from Thermochaetoides thermophila)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Thermochaetoides thermophila)
- Actin-related protein 8 (84 kDa, Protein from Thermochaetoides thermophila)
- Actin related protein 4 (arp4) (51 kDa, Protein from Thermochaetoides thermophila)
- Dna (26-mer) (70 kDa, DNA from synthetic construct)
- Actin (41 kDa, Protein from Thermochaetoides thermophila)
- Ino80 atpase (210 kDa, Protein from Thermochaetoides thermophila)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) on straight dna (Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Dna (26-mer) (69 kDa, DNA from synthetic construct)
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 8atf
Q-score: 0.474
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ DOI: doi:10.1126/sciadv.add3189 Pubmed: 36490333 ]
- Histone h3.2 (15 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Histone h2a (13 kDa, Protein from Homo sapiens)
- Dna (226-mer) (70 kDa, DNA from DNA molecule)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Ino80 atpase (131 kDa, Protein from Thermochaetoides thermophila)
- Papa-1 domain-containing protein (53 kDa, Protein from Thermochaetoides thermophila)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (227-mer) (69 kDa, DNA from DNA molecule)
- Chaetomium thermophilum ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Thermochaetoides thermophila)