Herdman M, von Kügelgen A, Kureisaite-Ciziene D, Duman R, El Omari K, Garman EF, Kjaer A, Kolokouris D, Löwe J, Wagner A, Stansfeld PJ, Bharat TAM
Structure (2021) [ DOI: 10.1016/j.str.2021.10.012 Pubmed: 34800371 ]
Image sets:
- Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours
- Gain reference image of the non-tilted and tilted dataset in MRC format
- Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt
- Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt (converted from .mrc into .tif with relion_convert_to_tiff)
- Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours (converted from .mrc into .tif with relion_convert_to_tiff)
Resolution: 4.37 Å
EM Method: Single-particle
Fitted PDBs: 7peo
Q-score: 0.298
Herdman M, von Kugelgen A, Kureisaite-Ciziene D, Duman R, El Omari K, Garman EF, Kjaer A, Kolokouris D, Lowe J, Wagner A, Stansfeld PJ, Bharat TAM
Structure (2022) 30 pp. 215- [ DOI: doi:10.1016/j.str.2021.10.012 Pubmed: 34800371 ]
- S-layer protein (25 kDa, Protein from Caulobacter vibrioides)
- Calcium ion (40 Da, Ligand)
- Holmium atom (164 Da, Ligand)
- Structure of the caulobacter crescentus s-layer protein rsaa n-terminal domain bound to lps and soaked with holmium (Complex from Caulobacter vibrioides)
In situ structure of the Caulobacter crescentus S-layer
von Kugelgen A, Tang H, Hardy GG, Kureisaite-Ciziene D, Brun YV, Stansfeld PJ, Robinson CV, Bharat TAM
Cell (2020) 180 pp. 348-358.e15 [ Pubmed: 31883796 DOI: doi:10.1016/j.cell.2019.12.006 ]
Structure of the RsaA N-terminal domain bound to LPS
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6t72
Q-score: 0.493
von Kugelgen A, Tang H, Hardy GG, Kureisaite-Ciziene D, Brun YV, Stansfeld PJ, Robinson CV, Bharat TAM
Cell (2020) 180 pp. 348-358.e15 [ Pubmed: 31883796 DOI: doi:10.1016/j.cell.2019.12.006 ]
- Calcium ion (40 Da, Ligand)
- S-layer protein (25 kDa, Protein from Caulobacter vibrioides CB15)
- Structure of rsaa n-terminal domain bound to lps (577 kDa, Complex from Caulobacter vibrioides CB15)
Cryo-EM reconstruction of Thermus thermophilus bactofilin double helical filaments
Resolution: 4.2 Å
EM Method: Helical reconstruction
Fitted PDBs: 6rib
Q-score: 0.363
Deng X, Gonzalez Llamazares A, Wagstaff JM, Hale VL, Cannone G, McLaughlin SH, Kureisaite-Ciziene D, Lowe J
Nat Microbiol (2019) 4 pp. 2357-2368 [ Pubmed: 31501539 DOI: doi:10.1038/s41564-019-0544-0 ]
Resolution: 3.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 5o09
Q-score: 0.276
Deng X, Fink G, Bharat TAM, He S, Kureisaite-Ciziene D, Lowe J
PNAS (2017) 114 pp. E5950-E5958 [ Pubmed: 28673988 DOI: doi:10.1073/pnas.1705062114 ]
- Btubabc mini microtubule (Complex from Prosthecobacter dejongeii)
- Bacterial kinesin light chain (27 kDa, Protein from Prosthecobacter vanneervenii)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin btubb (46 kDa, Protein from Prosthecobacter dejongeii)
- Tubulin (47 kDa, Protein from Prosthecobacter dejongeii)
- Btubabc mini microtubule (Complex from Prosthecobacter vanneervenii)
- Btubabc mini microtubule (Complex)
Structure of the C. crescentus S-layer
Resolution: 7.4 Å
EM Method: Subtomogram averaging
Fitted PDBs: 5n97
Q-score: 0.101
Bharat TAM, Kureisaite-Ciziene D, Hardy GG, Yu EW, Devant JM, Hagen WJH, Brun YV, Briggs JAG, Lowe J
Nat Microbiol (2017) 2 pp. 17059-17059 [ Pubmed: 28418382 DOI: doi:10.1038/nmicrobiol.2017.59 ]
- Caulobacter crescentus s-layer (Cellular component from Caulobacter crescentus NA1000)
- Calcium ion (40 Da, Ligand)
- S-layer protein rsaa (73 kDa, Protein from Caulobacter crescentus NA1000)
cryoEM structure of crenactin double helical filament at 3.8A resolution
Izore T, Kureisaite-Ciziene D, McLaughlin SH, Lowe J
eLife (2016) 5 [ Pubmed: 27852434 DOI: doi:10.7554/eLife.21600 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Crenactin (Complex from Pyrobaculum calidifontis)
- Actin/actin family protein (48 kDa, Protein from Pyrobaculum calidifontis (strain JCM 11548 / VA1))