Cryo-EM structure of BG505 Env mutant A517E in complex with antibody ACS202 Fab
Reveiz M, Xu K, Lee M, Wang S, Olia AS, Harris DR, Liu K, Liu T, Schaub AJ, Stephens T, Wang Y, Zhang B, Huang R, Tsybovsky Y, Kwong PD, Rawi R
Front Immunol (2024) 15 pp. 1484029-1484029 [ DOI: doi:10.3389/fimmu.2024.1484029 Pubmed: 39611147 ]
- Hiv-1 env trimer complexed with antibody fab (Complex from Human immunodeficiency virus 1)
- Envelope glycoprotein gp120 (53 kDa, Protein from Human immunodeficiency virus 1)
- Antibody acs202 fab light chain (23 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Envelope glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Antibody acs202 fab heavy chain (25 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Antibody fab (Complex from Homo sapiens)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8gav
Q-score: 0.605
Lederhofer J, Tsybovsky Y, Nguyen L, Raab JE, Creanga A, Stephens T, Gillespie RA, Syeda HZ, Fisher BE, Skertic M, Yap C, Schaub AJ, Rawi R, Kwong PD, Graham BS, McDermott AB, Andrews SF, King NP, Kanekiyo M
Immunity (2024) 57 pp. 574-586.e7 [ DOI: doi:10.1016/j.immuni.2024.02.003 Pubmed: 38430907 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Influenza virus neuraminidase from a/darwin/09/2021 (h3n2) in complex with fab nds.3 (380 kDa, Complex from Homo sapiens)
- Fab nds.3, heavy chain (24 kDa, Protein from Homo sapiens)
- Fab nds.3, light chain (22 kDa, Protein from Homo sapiens)
- Neuraminidase (51 kDa, Protein from Influenza A virus)
Vaccine-elicited human antibody 2C06 in complex with HIV-1 envelope trimer BG505 DS-SOSIP
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 8g4m
Q-score: 0.564
Wang S, Matassoli F, Zhang B, Liu T, Shen CH, Bylund T, Johnston T, Henry AR, Teng IT, Tripathi P, Becker JE, Changela A, Chaudhary R, Cheng C, Gaudinski M, Gorman J, Harris DR, Lee M, Morano NC, Novik L, O'Dell S, Olia AS, Parchment DK, Rawi R, Roberts-Torres J, Stephens T, Tsybovsky Y, Wang D, Van Wazer DJ, Zhou T, Doria-Rose NA, Koup RA, Shapiro L, Douek DC, McDermott AB, Kwong PD
Cell Rep (2023) 42 pp. 112755-112755 [ DOI: doi:10.1016/j.celrep.2023.112755 Pubmed: 37436899 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Envelope glycoprotein gp120 (52 kDa, Protein from Human immunodeficiency virus 1)
- Antibody light chain (23 kDa, Protein from Homo sapiens)
- Antibody heavy chain (25 kDa, Protein from Homo sapiens)
- Hiv envelope trimer in complex with three copies of antibody fab (Complex)
- Envelope glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
Vaccine-elicited human antibody 2C09 in complex with HIV-1 envelope trimer BG505 DS-SOSIP
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8g4t
Q-score: 0.56
Wang S, Matassoli F, Zhang B, Liu T, Shen CH, Bylund T, Johnston T, Henry AR, Teng IT, Tripathi P, Becker JE, Changela A, Chaudhary R, Cheng C, Gaudinski M, Gorman J, Harris DR, Lee M, Morano NC, Novik L, O'Dell S, Olia AS, Parchment DK, Rawi R, Roberts-Torres J, Stephens T, Tsybovsky Y, Wang D, Van Wazer DJ, Zhou T, Doria-Rose NA, Koup RA, Shapiro L, Douek DC, McDermott AB, Kwong PD
Cell Rep (2023) 42 pp. 112755-112755 [ DOI: doi:10.1016/j.celrep.2023.112755 Pubmed: 37436899 ]
- Fab 2c09 light chain (23 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fab 2c09 heavy chain (24 kDa, Protein from Homo sapiens)
- Hiv envelope trimer in complex with three copies of antibody fab (Complex)
- Envelope glycoprotein bg505 ds-sosip gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Envelope glycoprotein bg505 ds-sosip gp120 (52 kDa, Protein from Human immunodeficiency virus 1)
Antibody vFP53.02 in complex with HIV-1 envelope trimer BG505 DS-SOSIP
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 8fr6
Q-score: 0.528
Sastry M, Changela A, Gorman J, Xu K, Chuang GY, Shen CH, Cheng C, Geng H, O'Dell S, Ou L, Rawi R, Reveiz M, Stewart-Jones GBE, Wang S, Zhang B, Zhou T, Biju A, Chambers M, Chen X, Corrigan AR, Lin BC, Louder MK, McKee K, Nazzari AF, Olia AS, Parchment DK, Sarfo EK, Stephens T, Stuckey J, Tsybovsky Y, Verardi R, Wang Y, Zheng CY, Chen Y, Doria-Rose NA, McDermott AB, Mascola JR, Kwong PD
J Virol (2023) 97 pp. e0160422-e0160422 [ Pubmed: 37098956 DOI: doi:10.1128/jvi.01604-22 ]
- Antibody fab (Complex from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Envelope glycoprotein gp120 (52 kDa, Protein from Human immunodeficiency virus 1)
- Complex of antibody fab vfp53.02 with hiv-1 env trimer bg505 ds-sosip (Complex)
- Vfp53.02 fab heavy chain (24 kDa, Protein from Mus musculus)
- Hiv-1 env trimer bg505 ds-sosip (Complex from Human immunodeficiency virus 1)
- Vfp53.02 fab light chain (24 kDa, Protein from Mus musculus)
- Envelope glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
Cryo-EM structure of SARS-CoV-2 spike in complex with antibodies B1-182.1 and A19-61.1
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 7tb8
Q-score: 0.41
Zhou T, Wang L, Misasi J, Pegu A, Zhang Y, Harris DR, Olia AS, Talana CA, Yang ES, Chen M, Choe M, Shi W, Teng IT, Creanga A, Jenkins C, Leung K, Liu T, Stancofski ED, Stephens T, Zhang B, Tsybovsky Y, Graham BS, Mascola JR, Sullivan NJ, Kwong PD
Science (2022) 376 pp. eabn8897-eabn8897 [ Pubmed: 35324257 DOI: doi:10.1126/science.abn8897 ]
- The light chain of sars-cov-2 antibody b1-182.1 (11 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- The light chain of sars-cov-2 antibody a19-61.1 (11 kDa, Protein from Homo sapiens)
- The heavy chain of sars-cov-2 antibody a19-61.1 (13 kDa, Protein from Homo sapiens)
- Sars-cov-2 spike in complex with antibodies b1-182.1 and a19-61.1 (570 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- Spike glycoprotein (132 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- The heavy chain of sars-cov-2 antiboddy antibody b1-182.1 (13 kDa, Protein from Homo sapiens)
Structure of freshly purified SARS-CoV-2 S2P spike at pH 7.4
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7mtc
Q-score: 0.6
Olia AS, Tsybovsky Y, Chen SJ, Liu C, Nazzari AF, Ou L, Wang L, Kong WP, Leung K, Liu T, Stephens T, Teng IT, Wang S, Yang ES, Zhang B, Zhang Y, Zhou T, Mascola JR, Kwong PD
J Biol Chem (2021) 297 pp. 101127-101127 [ Pubmed: 34461095 DOI: doi:10.1016/j.jbc.2021.101127 ]
CryoEM structure of neutralizing nanobody Nb30 in complex with SARS-CoV2 spike
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 7my2
Q-score: 0.467
Xu J, Xu K, Jung S, Conte A, Lieberman J, Muecksch F, Lorenzi JCC, Park S, Schmidt F, Wang Z, Huang Y, Luo Y, Nair MS, Wang P, Schulz JE, Tessarollo L, Bylund T, Chuang GY, Olia AS, Stephens T, Teng IT, Tsybovsky Y, Zhou T, Munster V, Ho DD, Hatziioannou T, Bieniasz PD, Nussenzweig MC, Kwong PD, Casellas R
Nature (2021) 595 pp. 278-282 [ DOI: doi:10.1038/s41586-021-03676-z Pubmed: 34098567 ]
- Nanobody nb30 (14 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sars-cov2 spike in complex with nanobody nb30 (Complex from Severe acute respiratory syndrome coronavirus 2)
- Nanobody nb30 (Complex from Mus musculus)
- Sars-cov2 spike (Complex)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
CryoEM structure of neutralizing nanobody Nb12 in complex with SARS-CoV2 spike
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7my3
Q-score: 0.491
Xu J, Xu K, Jung S, Conte A, Lieberman J, Muecksch F, Lorenzi JCC, Park S, Schmidt F, Wang Z, Huang Y, Luo Y, Nair MS, Wang P, Schulz JE, Tessarollo L, Bylund T, Chuang GY, Olia AS, Stephens T, Teng IT, Tsybovsky Y, Zhou T, Munster V, Ho DD, Hatziioannou T, Bieniasz PD, Nussenzweig MC, Kwong PD, Casellas R
Nature (2021) 595 pp. 278-282 [ DOI: doi:10.1038/s41586-021-03676-z Pubmed: 34098567 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Nanobody nb12 (13 kDa, Protein from Mus musculus)
- Nanobody nb12 (Complex from Mus musculus)
- Sars-cov2 spike in complex with nanobody nb12 (Complex from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov2 spike (Complex)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Structure of SARS-CoV-2 spike at pH 4.5
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7jwy
Q-score: 0.612
Zhou T, Tsybovsky Y, Gorman J, Rapp M, Cerutti G, Chuang GY, Katsamba PS, Sampson JM, Schon A, Bimela J, Boyington JC, Nazzari A, Olia AS, Shi W, Sastry M, Stephens T, Stuckey J, Teng IT, Wang P, Wang S, Zhang B, Friesner RA, Ho DD, Mascola JR, Shapiro L, Kwong PD
Cell Host Microbe (2020) 28 pp. 867-879.e5 [ DOI: doi:10.1016/j.chom.2020.11.004 Pubmed: 33271067 ]
- Spike glycoprotein (140 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 spike at ph 4.5 (423 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Water (18 Da, Ligand)