Aquaporin Z with ALFA tag and bound to nanobody
Resolution: 1.9 Å
EM Method: Single-particle
Fitted PDBs: 8uy6
Q-score: 0.68
Stover L, Bahramimoghaddam H, Wang L, Schrecke S, Yadav GP, Zhou M, Laganowsky A
J Struct Biol X (2024) 9 pp. 100097-100097 [ DOI: doi:10.1016/j.yjsbx.2024.100097 Pubmed: 38361954 ]
- Cardiolipin (1 kDa, Ligand)
- Aquaporin z with alfa tag bound to nanobody (161 kDa, Complex)
- Nanobody (Complex from Vicugna pacos)
- Aquaporin z (26 kDa, Protein from Escherichia coli)
- Anti-alfa nanobody (13 kDa, Protein from Vicugna pacos)
- Aquaporin z with alfa tag (Complex from Escherichia coli)
KDL bound, nucleotide-free MsbA in open, outward-facing conformation
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 8tso
Q-score: 0.59
Zhang T, Lyu J, Yang B, Yun SD, Scott E, Zhao M, Laganowsky A
Nat Commun (2024) 15 pp. 5946-5946 [ Pubmed: 39009687 DOI: doi:10.1038/s41467-024-50350-9 ]
- Atp-binding transport protein msba (64 kDa, Protein from Escherichia coli)
- Pentaethylene glycol monodecyl ether (378 Da, Ligand)
- (2~{r},4~{r},5~{r},6~{r})-6-[(1~{r})-1,2-bis(oxidanyl)ethyl]-2-[(2~{r},4~{r},5~{r},6~{r})-6-[(1~{r})-1,2-bis(oxidanyl)ethyl]-2-carboxy-2-[[(2~{r},3~{s},4~{r},5~{r},6~{r})-5-[[(3~{r})-3-dodecanoyloxytetradecanoyl]amino]-6-[[(2~{r},3~{s},4~{r},5~{r},6~{r})-3-oxidanyl-5-[[(3~{r})-3-oxidanyltetradecanoyl]amino]-4-[(3~{r})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(3~{r})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]methoxy]-5-oxidanyl-oxan-4-yl]oxy-4,5-bis(oxidanyl)oxane-2-carboxylic acid (2 kDa, Ligand)
- E. coli msba incubated with atp for 6 hours (Complex from Escherichia coli)
Open, inward-facing MsbA structure (OIF1)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8tsp
Q-score: 0.381
Zhang T, Lyu J, Yang B, Yun SD, Scott E, Zhao M, Laganowsky A
Nat Commun (2024) 15 pp. 5946-5946 [ Pubmed: 39009687 DOI: doi:10.1038/s41467-024-50350-9 ]
Open, inward-facing MsbA structure (OIF2)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8tsq
Q-score: 0.424
Zhang T, Lyu J, Yang B, Yun SD, Scott E, Zhao M, Laganowsky A
Nat Commun (2024) 15 pp. 5946-5946 [ Pubmed: 39009687 DOI: doi:10.1038/s41467-024-50350-9 ]
Open, inward-facing MsbA structure (OIF4)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8tsr
Q-score: 0.37
Zhang T, Lyu J, Yang B, Yun SD, Scott E, Zhao M, Laganowsky A
Nat Commun (2024) 15 pp. 5946-5946 [ Pubmed: 39009687 DOI: doi:10.1038/s41467-024-50350-9 ]
Open, inward-facing MsbA structure (OIF3)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8tss
Q-score: 0.392
Zhang T, Lyu J, Yang B, Yun SD, Scott E, Zhao M, Laganowsky A
Nat Commun (2024) 15 pp. 5946-5946 [ Pubmed: 39009687 DOI: doi:10.1038/s41467-024-50350-9 ]
Structure of the vanadate-trapped MsbA bound to KDL
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 8dmm
Q-score: 0.45
Lyu J, Liu C, Zhang T, Schrecke S, Elam NP, Packianathan C, Hochberg GKA, Russell D, Zhao M, Laganowsky A
Nat Commun (2022) 13 pp. 7291-7291 [ Pubmed: 36435815 DOI: doi:10.1038/s41467-022-34905-2 ]
- Atp-binding transport protein msba (64 kDa, Protein from Escherichia coli)
- Adp orthovanadate (544 Da, Ligand)
- (2~{r},4~{r},5~{r},6~{r})-6-[(1~{r})-1,2-bis(oxidanyl)ethyl]-2-[(2~{r},4~{r},5~{r},6~{r})-6-[(1~{r})-1,2-bis(oxidanyl)ethyl]-2-carboxy-2-[[(2~{r},3~{s},4~{r},5~{r},6~{r})-5-[[(3~{r})-3-dodecanoyloxytetradecanoyl]amino]-6-[[(2~{r},3~{s},4~{r},5~{r},6~{r})-3-oxidanyl-5-[[(3~{r})-3-oxidanyltetradecanoyl]amino]-4-[(3~{r})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(3~{r})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]methoxy]-5-oxidanyl-oxan-4-yl]oxy-4,5-bis(oxidanyl)oxane-2-carboxylic acid (2 kDa, Ligand)
- Vanadate-trapped msba kdl and adp orthovanadate (aov) (Complex from Escherichia coli)
Structure of open, inward-facing MsbA from E. coli
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8dmo
Q-score: 0.307
Lyu J, Liu C, Zhang T, Schrecke S, Elam NP, Packianathan C, Hochberg GKA, Russell D, Zhao M, Laganowsky A
Nat Commun (2022) 13 pp. 7291-7291 [ Pubmed: 36435815 DOI: doi:10.1038/s41467-022-34905-2 ]
- Copper-bound msba (Complex from Escherichia coli)
- Atp-binding transport protein msba (64 kDa, Protein from Escherichia coli)