Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 9bh8
Q-score: 0.401
Zerio CJ, Bai Y, Sosa-Alvarado BA, Guzi T, Lander GC
To Be Published
- Dna polymerase theta (99 kDa, Protein from Homo sapiens)
- Human dna polymerase theta helicase domain bound to stem-loop dna with microhomology in the 3' overhang (20 kDa, Complex from Homo sapiens)
- Stem-loop dna with microhomology in the 3' overhang (17 kDa, DNA from synthetic construct)
- Human dna polymerase theta helicase domain (Complex from Homo sapiens)
- Stem-loop dna with microhomology in the 3' overhang (Complex from Homo sapiens)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9bh9
Q-score: 0.422
Zerio CJ, Bai Y, Sosa-Alvarado BA, Guzi T, Lander GC
To Be Published
- Dna polymerase theta (99 kDa, Protein from Homo sapiens)
- Human dna polymerase theta helicase domain bound to stem-loop dna with microhomology in the 3' overhang (20 kDa, Complex from Homo sapiens)
- Stem-loop dna with microhomology in the 3' overhang (17 kDa, DNA from synthetic construct)
- Human dna polymerase theta helicase domain (Complex from Homo sapiens)
- Stem-loop dna with microhomology in the 3' overhang (Complex from Homo sapiens)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9bha
Q-score: 0.43
Zerio CJ, Bai Y, Sosa-Alvarado BA, Guzi T, Lander GC
To Be Published
- Dna polymerase theta (99 kDa, Protein from Homo sapiens)
- Human dna polymerase theta helicase domain bound to stem-loop dna with microhomology in the 3' overhang (20 kDa, Complex from Homo sapiens)
- Stem-loop dna with microhomology in the 3' overhang (17 kDa, DNA from synthetic construct)
- Human dna polymerase theta helicase domain (Complex from Homo sapiens)
- Stem-loop dna with microhomology in the 3' overhang (Complex from Homo sapiens)
Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 8flj
Q-score: 0.35
Santiago-Frangos A, Henriques WS, Wiegand T, Gauvin CC, Buyukyoruk M, Graham AB, Wilkinson RA, Triem L, Neselu K, Eng ET, Lander GC, Wiedenheft B
Nat Struct Mol Biol (2023) 30 pp. 1675-1685 [ DOI: doi:10.1038/s41594-023-01097-2 Pubmed: 37710013 ]
- Crispr repeat and prespacer, sense strand of dna (18 kDa, DNA from synthetic construct)
- Cas1-cas2/3 heterohexameric integrase (Complex from Pseudomonas aeruginosa PA14)
- Crispr-associated nuclease/helicase cas3 subtype i-f/ypest (121 kDa, Protein from Pseudomonas aeruginosa PA14)
- Integration host factor subunit alpha (11 kDa, Protein from Pseudomonas aeruginosa PA14)
- Crispr leader, repeat, and prespacer dna annealed to mimic a half-site integration intermediate. (Complex)
- Ihf heterodimer (Complex from Pseudomonas aeruginosa PA14)
- Cas1-cas2/3 integrase and ihf bound to crispr leader, repeat and foreign dna (122 kDa, Complex)
- Integration host factor subunit beta (10 kDa, Protein from Pseudomonas aeruginosa PA14)
- Crispr leader, sense strand of dna (42 kDa, DNA from Pseudomonas aeruginosa PA14)
- Crispr leader and repeat, anti-sense strand of dna (52 kDa, DNA from Pseudomonas aeruginosa PA14)
- Crispr-associated endonuclease cas1 (38 kDa, Protein from Pseudomonas aeruginosa PA14)
- Prespacer, anti-sense strand of dna (8 kDa, DNA from synthetic construct)
Cryo-EM structure of Ephydatia fluviatilis PiwiA-piRNA complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7kx7
Q-score: 0.457
Anzelon TA, Chowdhury S, Hughes SM, Xiao Y, Lander GC, MacRae IJ
Nature (2021) 597 pp. 285-289 [ Pubmed: 34471284 DOI: doi:10.1038/s41586-021-03856-x ]
- Complex between efpiwi and pirna (83 kDa, Complex)
- Piwi-a (88 kDa, Protein from Ephydatia fluviatilis)
- Piwi-a (Complex from Ephydatia fluviatilis)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(p*up*cp*up*cp*ap*gp*(omc))-3') (8 kDa, RNA from synthetic construct)
- Pirna (Complex from synthetic construct)
Cryo-EM structure of EfPiwiA(MID/PIWI domains)-piRNA-long-target complex
Anzelon TA, Chowdhury S, Hughes SM, Xiao Y, Lander GC, MacRae IJ
Nature (2021) 597 pp. 285-289 [ Pubmed: 34471284 DOI: doi:10.1038/s41586-021-03856-x ]
Cryo-EM structure of Ephydatia fluviatilis PiwiA-piRNA-target complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7kx9
Q-score: 0.461
Anzelon TA, Chowdhury S, Hughes SM, Xiao Y, Lander GC, MacRae IJ
Nature (2021) 597 pp. 285-289 [ Pubmed: 34471284 DOI: doi:10.1038/s41586-021-03856-x ]
- Rna (5'-r(p*up*cp*up*cp*up*up*gp*ap*gp*up*up*gp*gp*ap*cp*ap*ap*ap*up*gp*gp*cp*ap*(omg))-3') (7 kDa, RNA from synthetic construct)
- Pirna-target (Complex from synthetic construct)
- Piwi-a (88 kDa, Protein from Ephydatia fluviatilis)
- Piwi-a (Complex from Ephydatia fluviatilis)
- Efpiwi-pirna-target complex (94 kDa, Complex)
- Rna (5'-r(p*up*gp*up*cp*cp*ap*ap*cp*up*cp*ap*ap*gp*ap*gp*a)-3') (5 kDa, RNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)