Cryo-EM structure of LKB1-STRADalpha-MO25alpha heterocomplex
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 8vsu
Q-score: 0.575
Chan LM, Courteau BJ, Maker A, Wu M, Basanta B, Mehmood H, Bulkley D, Joyce D, Lee BC, Mick S, Czarnik C, Gulati S, Lander GC, Verba KA
J Struct Biol (2024) 216 pp. 108108-108108 [ Pubmed: 38944401 DOI: doi:10.1016/j.jsb.2024.108108 ]
- Isoform 3 of ste20-related kinase adapter protein alpha (47 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Serine/threonine-protein kinase stk11 (50 kDa, Protein from Homo sapiens)
- Calcium-binding protein 39 (41 kDa, Protein from Homo sapiens)
- Heterotrimeric complex of serine/threonine kinase lkb1 with psuedokinase strada and scaffolding mo25a (140 kDa, Complex from Homo sapiens)
Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 8flj
Q-score: 0.35
Santiago-Frangos A, Henriques WS, Wiegand T, Gauvin CC, Buyukyoruk M, Graham AB, Wilkinson RA, Triem L, Neselu K, Eng ET, Lander GC, Wiedenheft B
Nat Struct Mol Biol (2023) 30 pp. 1675-1685 [ Pubmed: 37710013 DOI: doi:10.1038/s41594-023-01097-2 ]
- Crispr leader, sense strand of dna (42 kDa, DNA from Pseudomonas aeruginosa PA14)
- Crispr-associated nuclease/helicase cas3 subtype i-f/ypest (121 kDa, Protein from Pseudomonas aeruginosa PA14)
- Integration host factor subunit alpha (11 kDa, Protein from Pseudomonas aeruginosa PA14)
- Ihf heterodimer (Complex from Pseudomonas aeruginosa PA14)
- Integration host factor subunit beta (10 kDa, Protein from Pseudomonas aeruginosa PA14)
- Crispr-associated endonuclease cas1 (38 kDa, Protein from Pseudomonas aeruginosa PA14)
- Crispr leader, repeat, and prespacer dna annealed to mimic a half-site integration intermediate. (Complex)
- Crispr repeat and prespacer, sense strand of dna (18 kDa, DNA from synthetic construct)
- Crispr leader and repeat, anti-sense strand of dna (52 kDa, DNA from Pseudomonas aeruginosa PA14)
- Cas1-cas2/3 heterohexameric integrase (Complex from Pseudomonas aeruginosa PA14)
- Cas1-cas2/3 integrase and ihf bound to crispr leader, repeat and foreign dna (122 kDa, Complex)
- Prespacer, anti-sense strand of dna (8 kDa, DNA from synthetic construct)
Lon Protease from Yersinia pestis
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6v11
Q-score: 0.405
Shin M, Puchades C, Asmita A, Puri N, Adjei E, Wiseman RL, Karzai AW, Lander GC
Sci Adv (2020) 6 pp. eaba8404-eaba8404 [ DOI: doi:10.1126/sciadv.aba8404 Pubmed: 32490208 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon protease from yersinia pestis (Complex from Yersinia pestis)
- Lon protease (57 kDa, Protein from Yersinia pestis)
human m-AAA protease AFG3L2, substrate-bound
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6nyy
Q-score: 0.481
Puchades C, Ding B, Song A, Wiseman RL, Lander GC, Glynn SE
Mol Cell (2019) 75 pp. 1073- [ DOI: doi:10.1016/j.molcel.2019.06.016 Pubmed: 31327635 ]
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Afg3l2 (360 kDa, Cellular component from Homo sapiens)
- Afg3-like protein 2 (58 kDa, Protein from Homo sapiens)
- Substrate (799 Da, Protein from Homo sapiens)
Lon Protease from Yersinia pestis with Y2853 substrate
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6on2
Q-score: 0.537
Shin M, Puchades C, Asmita A, Puri N, Adjei E, Wiseman RL, Karzai AW, Lander GC
Sci Adv (2020) 6 pp. eaba8404-eaba8404 [ DOI: doi:10.1126/sciadv.aba8404 Pubmed: 32490208 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Bound y2853 substrate (515 Da, Protein from Yersinia pestis)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon protease bound to y2853 substrate (Complex from Yersinia pestis)
- Atp-dependent protease la (57 kDa, Protein from Yersinia pestis)
Mitochondrial ATPase Protease YME1
Puchades C, Rampello AJ, Shin M, Giuliano CJ, Wiseman RL, Glynn SE, Lander GC
Science (2017) 358 [ Pubmed: 29097521 DOI: doi:10.1126/science.aao0464 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Poly(unk) (869 Da, Protein from Escherichia coli)
- Yme1 atpase protease walker b mutant bound to substrate (295 kDa, Complex from Saccharomyces cerevisiae)
- Mitochondrial inner membrane i-aaa protease supercomplex subunit yme1 (48 kDa, Protein from Saccharomyces cerevisiae (strain RM11-1a))
Chowdhury S, Carter J, Rollins MF, Golden SM, Jackson RN, Hoffmann C, Nosaka L, Bondy-Denomy J, Maxwell KL, Davidson AR, Fischer ER, Lander GC, Wiedenheft B
Cell (2017) 169 pp. 47-57.e11 [ DOI: doi:10.1016/j.cell.2017.03.012 Pubmed: 28340349 ]
- Anti-crispr protein 30 (10 kDa, Protein from Pseudomonas phage D3112)
- Crispr-associated protein csy2 (36 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crispr rna (60-mer) (19 kDa, RNA from Pseudomonas aeruginosa UCBPP-PA14)
- Anti-crisprs acrf1 and acrf2 bound p. aeruginosa crrna-guided crispr surveillance(csy)complex. (450 kDa, Complex from Pseudomonas aeruginosa UCBPP-PA14)
- Crispr-associated protein csy3 (37 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crispr-associated protein csy1 (49 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Anti-crispr protein acr30-35 (8 kDa, Protein from Pseudomonas phage JBD30)