Yeast Lon (PIM1) with endogenous substrate
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7sxo
Q-score: 0.471
Yang J, Song AS, Wiseman RL, Lander GC
J Biol Chem (2022) 298 pp. 101694-101694 [ DOI: doi:10.1016/j.jbc.2022.101694 Pubmed: 35143841 ]
- Cryo-em structure of yeast lon (pim1) in complex with endogenous substrate (Complex from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Endogenous substrate (1 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon protease homolog, mitochondrial (108 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Human mitochondrial LONP1 in complex with Bortezomib
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7krz
Q-score: 0.572
Shin M, Watson ER, Song AS, Mindrebo JT, Novick SJ, Griffin PR, Wiseman RL, Lander GC
Nat Commun (2021) 12 pp. 3239-3239 [ Pubmed: 34050165 DOI: doi:10.1038/s41467-021-23495-0 ]
- Endogenous co-purified substrate (1 kDa, Protein from Escherichia coli BL21(DE3))
- Lon protease homolog, mitochondrial (59 kDa, Protein from Homo sapiens)
- Human mitochondrial lonp1 in complex with bortezomib (462 kDa, Complex from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- N-[(1r)-1-(dihydroxyboryl)-3-methylbutyl]-n-(pyrazin-2-ylcarbonyl)-l-phenylalaninamide (384 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
WalkerB mutant human mitochondrial LONP1 bound to endogenous substrate
Shin M, Watson ER, Song AS, Mindrebo JT, Novick SJ, Griffin PR, Wiseman RL, Lander GC
Nat Commun (2021) 12 pp. 3239-3239 [ Pubmed: 34050165 DOI: doi:10.1038/s41467-021-23495-0 ]
Substrate-free human mitochondrial LONP1
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7ksl
Q-score: 0.506
Shin M, Watson ER, Song AS, Mindrebo JT, Novick SJ, Griffin PR, Wiseman RL, Lander GC
Nat Commun (2021) 12 pp. 3239-3239 [ DOI: doi:10.1038/s41467-021-23495-0 Pubmed: 34050165 ]
- Lon protease homolog, mitochondrial (58 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Substrate-free human mitochondrial lonp1 (462 kDa, Complex from Homo sapiens)
Human mitochondrial LONP1 with endogenous substrate
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7ksm
Q-score: 0.568
Shin M, Watson ER, Song AS, Mindrebo JT, Novick SJ, Griffin PR, Wiseman RL, Lander GC
Nat Commun (2021) 12 pp. 3239-3239 [ Pubmed: 34050165 DOI: doi:10.1038/s41467-021-23495-0 ]
- Unidentified endogenous substrate (1 kDa, Protein from Escherichia coli BL21(DE3))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Lon protease homolog, mitochondrial (59 kDa, Protein from Homo sapiens)
- Human mitochondrial lonp1 with endogenous substrate (462 kDa, Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Lon Protease from Yersinia pestis
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6v11
Q-score: 0.405
Shin M, Puchades C, Asmita A, Puri N, Adjei E, Wiseman RL, Karzai AW, Lander GC
Sci Adv (2020) 6 pp. eaba8404-eaba8404 [ DOI: doi:10.1126/sciadv.aba8404 Pubmed: 32490208 ]
- Lon protease (57 kDa, Protein from Yersinia pestis)
- Lon protease from yersinia pestis (Complex from Yersinia pestis)
- Adenosine-5'-diphosphate (427 Da, Ligand)
human m-AAA protease AFG3L2, substrate-bound
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6nyy
Q-score: 0.481
Puchades C, Ding B, Song A, Wiseman RL, Lander GC, Glynn SE
Mol Cell (2019) 75 pp. 1073- [ DOI: doi:10.1016/j.molcel.2019.06.016 Pubmed: 31327635 ]
- Afg3-like protein 2 (58 kDa, Protein from Homo sapiens)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Substrate (799 Da, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Afg3l2 (360 kDa, Cellular component from Homo sapiens)
Lon Protease from Yersinia pestis with Y2853 substrate
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6on2
Q-score: 0.537
Shin M, Puchades C, Asmita A, Puri N, Adjei E, Wiseman RL, Karzai AW, Lander GC
Sci Adv (2020) 6 pp. eaba8404-eaba8404 [ DOI: doi:10.1126/sciadv.aba8404 Pubmed: 32490208 ]
- Bound y2853 substrate (515 Da, Protein from Yersinia pestis)
- Atp-dependent protease la (57 kDa, Protein from Yersinia pestis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Lon protease bound to y2853 substrate (Complex from Yersinia pestis)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Mitochondrial ATPase Protease YME1
Puchades C, Rampello AJ, Shin M, Giuliano CJ, Wiseman RL, Glynn SE, Lander GC
Science (2017) 358 [ Pubmed: 29097521 DOI: doi:10.1126/science.aao0464 ]
- Yme1 atpase protease walker b mutant bound to substrate (295 kDa, Complex from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Poly(unk) (869 Da, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Mitochondrial inner membrane i-aaa protease supercomplex subunit yme1 (48 kDa, Protein from Saccharomyces cerevisiae (strain RM11-1a))
- Adenosine-5'-diphosphate (427 Da, Ligand)