Yeast Lon (PIM1) with endogenous substrate
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7sxo
Q-score: 0.471
Yang J, Song AS, Wiseman RL, Lander GC
J Biol Chem (2022) 298 pp. 101694-101694 [ Pubmed: 35143841 DOI: doi:10.1016/j.jbc.2022.101694 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Cryo-em structure of yeast lon (pim1) in complex with endogenous substrate (Complex from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Endogenous substrate (1 kDa, Protein from Escherichia coli)
- Lon protease homolog, mitochondrial (108 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Human mitochondrial LONP1 in complex with Bortezomib
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7krz
Q-score: 0.572
Shin M, Watson ER, Song AS, Mindrebo JT, Novick SJ, Griffin PR, Wiseman RL, Lander GC
Nat Commun (2021) 12 pp. 3239-3239 [ DOI: doi:10.1038/s41467-021-23495-0 Pubmed: 34050165 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon protease homolog, mitochondrial (59 kDa, Protein from Homo sapiens)
- N-[(1r)-1-(dihydroxyboryl)-3-methylbutyl]-n-(pyrazin-2-ylcarbonyl)-l-phenylalaninamide (384 Da, Ligand)
- Human mitochondrial lonp1 in complex with bortezomib (462 kDa, Complex from Homo sapiens)
- Endogenous co-purified substrate (1 kDa, Protein from Escherichia coli BL21(DE3))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
WalkerB mutant human mitochondrial LONP1 bound to endogenous substrate
Shin M, Watson ER, Song AS, Mindrebo JT, Novick SJ, Griffin PR, Wiseman RL, Lander GC
Nat Commun (2021) 12 pp. 3239-3239 [ DOI: doi:10.1038/s41467-021-23495-0 Pubmed: 34050165 ]
Substrate-free human mitochondrial LONP1
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7ksl
Q-score: 0.506
Shin M, Watson ER, Song AS, Mindrebo JT, Novick SJ, Griffin PR, Wiseman RL, Lander GC
Nat Commun (2021) 12 pp. 3239-3239 [ DOI: doi:10.1038/s41467-021-23495-0 Pubmed: 34050165 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Substrate-free human mitochondrial lonp1 (462 kDa, Complex from Homo sapiens)
- Lon protease homolog, mitochondrial (58 kDa, Protein from Homo sapiens)
Human mitochondrial LONP1 with endogenous substrate
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7ksm
Q-score: 0.568
Shin M, Watson ER, Song AS, Mindrebo JT, Novick SJ, Griffin PR, Wiseman RL, Lander GC
Nat Commun (2021) 12 pp. 3239-3239 [ DOI: doi:10.1038/s41467-021-23495-0 Pubmed: 34050165 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon protease homolog, mitochondrial (59 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Unidentified endogenous substrate (1 kDa, Protein from Escherichia coli BL21(DE3))
- Human mitochondrial lonp1 with endogenous substrate (462 kDa, Complex from Homo sapiens)
Lon Protease from Yersinia pestis
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6v11
Q-score: 0.405
Shin M, Puchades C, Asmita A, Puri N, Adjei E, Wiseman RL, Karzai AW, Lander GC
Sci Adv (2020) 6 pp. eaba8404-eaba8404 [ Pubmed: 32490208 DOI: doi:10.1126/sciadv.aba8404 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon protease from yersinia pestis (Complex from Yersinia pestis)
- Lon protease (57 kDa, Protein from Yersinia pestis)
human m-AAA protease AFG3L2, substrate-bound
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6nyy
Q-score: 0.481
Puchades C, Ding B, Song A, Wiseman RL, Lander GC, Glynn SE
Mol Cell (2019) 75 pp. 1073- [ DOI: doi:10.1016/j.molcel.2019.06.016 Pubmed: 31327635 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Afg3-like protein 2 (58 kDa, Protein from Homo sapiens)
- Afg3l2 (360 kDa, Cellular component from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Substrate (799 Da, Protein from Homo sapiens)
Lon Protease from Yersinia pestis with Y2853 substrate
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6on2
Q-score: 0.537
Shin M, Puchades C, Asmita A, Puri N, Adjei E, Wiseman RL, Karzai AW, Lander GC
Sci Adv (2020) 6 pp. eaba8404-eaba8404 [ Pubmed: 32490208 DOI: doi:10.1126/sciadv.aba8404 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon protease bound to y2853 substrate (Complex from Yersinia pestis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent protease la (57 kDa, Protein from Yersinia pestis)
- Bound y2853 substrate (515 Da, Protein from Yersinia pestis)
Mitochondrial ATPase Protease YME1
Puchades C, Rampello AJ, Shin M, Giuliano CJ, Wiseman RL, Glynn SE, Lander GC
Science (2017) 358 [ DOI: doi:10.1126/science.aao0464 Pubmed: 29097521 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mitochondrial inner membrane i-aaa protease supercomplex subunit yme1 (48 kDa, Protein from Saccharomyces cerevisiae (strain RM11-1a))
- Zinc ion (65 Da, Ligand)
- Poly(unk) (869 Da, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Yme1 atpase protease walker b mutant bound to substrate (295 kDa, Complex from Saccharomyces cerevisiae)