Yeast Lon (PIM1) with endogenous substrate
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7sxo
Q-score: 0.471
Yang J, Song AS, Wiseman RL, Lander GC
J Biol Chem (2022) 298 pp. 101694-101694 [ Pubmed: 35143841 DOI: doi:10.1016/j.jbc.2022.101694 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Cryo-em structure of yeast lon (pim1) in complex with endogenous substrate (Complex from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Endogenous substrate (1 kDa, Protein from Escherichia coli)
- Lon protease homolog, mitochondrial (108 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Human mitochondrial LONP1 in complex with Bortezomib
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7krz
Q-score: 0.572
Shin M, Watson ER, Song AS, Mindrebo JT, Novick SJ, Griffin PR, Wiseman RL, Lander GC
Nat Commun (2021) 12 pp. 3239-3239 [ DOI: doi:10.1038/s41467-021-23495-0 Pubmed: 34050165 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon protease homolog, mitochondrial (59 kDa, Protein from Homo sapiens)
- N-[(1r)-1-(dihydroxyboryl)-3-methylbutyl]-n-(pyrazin-2-ylcarbonyl)-l-phenylalaninamide (384 Da, Ligand)
- Human mitochondrial lonp1 in complex with bortezomib (462 kDa, Complex from Homo sapiens)
- Endogenous co-purified substrate (1 kDa, Protein from Escherichia coli BL21(DE3))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Substrate-free human mitochondrial LONP1
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7ksl
Q-score: 0.506
Shin M, Watson ER, Song AS, Mindrebo JT, Novick SJ, Griffin PR, Wiseman RL, Lander GC
Nat Commun (2021) 12 pp. 3239-3239 [ DOI: doi:10.1038/s41467-021-23495-0 Pubmed: 34050165 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Substrate-free human mitochondrial lonp1 (462 kDa, Complex from Homo sapiens)
- Lon protease homolog, mitochondrial (58 kDa, Protein from Homo sapiens)
Human mitochondrial LONP1 with endogenous substrate
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7ksm
Q-score: 0.568
Shin M, Watson ER, Song AS, Mindrebo JT, Novick SJ, Griffin PR, Wiseman RL, Lander GC
Nat Commun (2021) 12 pp. 3239-3239 [ DOI: doi:10.1038/s41467-021-23495-0 Pubmed: 34050165 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon protease homolog, mitochondrial (59 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Unidentified endogenous substrate (1 kDa, Protein from Escherichia coli BL21(DE3))
- Human mitochondrial lonp1 with endogenous substrate (462 kDa, Complex from Homo sapiens)
Lon Protease from Yersinia pestis
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6v11
Q-score: 0.405
Shin M, Puchades C, Asmita A, Puri N, Adjei E, Wiseman RL, Karzai AW, Lander GC
Sci Adv (2020) 6 pp. eaba8404-eaba8404 [ Pubmed: 32490208 DOI: doi:10.1126/sciadv.aba8404 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon protease from yersinia pestis (Complex from Yersinia pestis)
- Lon protease (57 kDa, Protein from Yersinia pestis)
human m-AAA protease AFG3L2, substrate-bound
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6nyy
Q-score: 0.481
Puchades C, Ding B, Song A, Wiseman RL, Lander GC, Glynn SE
Mol Cell (2019) 75 pp. 1073- [ DOI: doi:10.1016/j.molcel.2019.06.016 Pubmed: 31327635 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Afg3-like protein 2 (58 kDa, Protein from Homo sapiens)
- Afg3l2 (360 kDa, Cellular component from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Substrate (799 Da, Protein from Homo sapiens)
Lon Protease from Yersinia pestis with Y2853 substrate
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6on2
Q-score: 0.537
Shin M, Puchades C, Asmita A, Puri N, Adjei E, Wiseman RL, Karzai AW, Lander GC
Sci Adv (2020) 6 pp. eaba8404-eaba8404 [ Pubmed: 32490208 DOI: doi:10.1126/sciadv.aba8404 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon protease bound to y2853 substrate (Complex from Yersinia pestis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent protease la (57 kDa, Protein from Yersinia pestis)
- Bound y2853 substrate (515 Da, Protein from Yersinia pestis)
Mitochondrial ATPase Protease YME1
Puchades C, Rampello AJ, Shin M, Giuliano CJ, Wiseman RL, Glynn SE, Lander GC
Science (2017) 358 [ DOI: doi:10.1126/science.aao0464 Pubmed: 29097521 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mitochondrial inner membrane i-aaa protease supercomplex subunit yme1 (48 kDa, Protein from Saccharomyces cerevisiae (strain RM11-1a))
- Zinc ion (65 Da, Ligand)
- Poly(unk) (869 Da, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Yme1 atpase protease walker b mutant bound to substrate (295 kDa, Complex from Saccharomyces cerevisiae)