Single Particle cryoEM dataset of Type I-F CRISPR integration complex from Pseudomonas aeruginosa
Santiago-Frangos A, Henriques WS, Wiegand T, Gauvin CC, Buyukyoruk M, Graham AB, Wilkinson RA, Triem L, Neselu K, Eng ET, Lander GC, Wiedenheft B
Nat Struct Mol Biol (2023) [ DOI: 10.1038/s41594-023-01097-2 ]
Image sets:
Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 8flj
Q-score: 0.35
Santiago-Frangos A, Henriques WS, Wiegand T, Gauvin CC, Buyukyoruk M, Graham AB, Wilkinson RA, Triem L, Neselu K, Eng ET, Lander GC, Wiedenheft B
Nat Struct Mol Biol (2023) 30 pp. 1675-1685 [ DOI: doi:10.1038/s41594-023-01097-2 Pubmed: 37710013 ]
- Crispr leader and repeat, anti-sense strand of dna (52 kDa, DNA from Pseudomonas aeruginosa PA14)
- Ihf heterodimer (Complex from Pseudomonas aeruginosa PA14)
- Cas1-cas2/3 integrase and ihf bound to crispr leader, repeat and foreign dna (122 kDa, Complex)
- Crispr-associated endonuclease cas1 (38 kDa, Protein from Pseudomonas aeruginosa PA14)
- Integration host factor subunit beta (10 kDa, Protein from Pseudomonas aeruginosa PA14)
- Cas1-cas2/3 heterohexameric integrase (Complex from Pseudomonas aeruginosa PA14)
- Crispr-associated nuclease/helicase cas3 subtype i-f/ypest (121 kDa, Protein from Pseudomonas aeruginosa PA14)
- Integration host factor subunit alpha (11 kDa, Protein from Pseudomonas aeruginosa PA14)
- Crispr leader, sense strand of dna (42 kDa, DNA from Pseudomonas aeruginosa PA14)
- Prespacer, anti-sense strand of dna (8 kDa, DNA from synthetic construct)
- Crispr repeat and prespacer, sense strand of dna (18 kDa, DNA from synthetic construct)
- Crispr leader, repeat, and prespacer dna annealed to mimic a half-site integration intermediate. (Complex)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6w1x
Q-score: 0.36
Hirschi M, Lu WT, Santiago-Frangos A, Wilkinson R, Golden SM, Davidson AR, Lander GC, Wiedenheft B
Nat Commun (2020) 11 pp. 2730-2730 [ Pubmed: 32483187 DOI: doi:10.1038/s41467-020-16512-1 ]
- Crispr-associated protein csy1 (49 kDa, Protein from Pseudomonas aeruginosa)
- Anti-crispr acrif9 bound to the type i-f crrna-guided crispr surveillance complex (Complex)
- Anti-crispr acrif9 (7 kDa, Protein from Proteus penneri)
- Type i-f crrna-guided crispr surveillance complex (Complex from Pseudomonas aeruginosa)
- Anti-crispr acrif9 (Complex from Proteus penneri)
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa)
- Rna (60-mer) (19 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (39 kDa, Protein from Pseudomonas aeruginosa)
- Type i-f crispr-associated protein csy2 (36 kDa, Protein from Pseudomonas aeruginosa)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6whi
Q-score: 0.373
Hirschi M, Lu WT, Santiago-Frangos A, Wilkinson R, Golden SM, Davidson AR, Lander GC, Wiedenheft B
Nat Commun (2020) 11 pp. 2730-2730 [ Pubmed: 32483187 DOI: doi:10.1038/s41467-020-16512-1 ]
- Anti-crispr acrif9 (Protein from Proteus penneri)
- Crispr-associated protein csy3 (Protein from Pseudomonas aeruginosa)
- Crispr-associated endonuclease cas6/csy4 (Protein from Pseudomonas aeruginosa)
- Type i-f crispr-associated protein csy2 (Protein from Pseudomonas aeruginosa)
- Anti-crispr acrif9 bound to the type i-f crrna-guided crispr surveillance complex (Complex)
- Crispr-associated protein csy1 (Protein from Pseudomonas aeruginosa)
- Non-target dsdna (Protein from Pseudomonas aeruginosa)
- Anti-crispr acrif9 (Complex from Proteus penneri)
- Type i-f crrna-guided crispr surveillance complex (Complex from Pseudomonas aeruginosa)
- Rna (60-mer) (Protein from Pseudomonas aeruginosa)