Cryo-EM structure of S. cerevisiae PolE-core-DNA
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9b8r
Q-score: 0.332
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ DOI: doi:10.1126/science.adk5901 Pubmed: 39088616 ]
- Primer dna (3 kDa, DNA from DNA molecule)
- Pole-core-dna complex (Complex from Saccharomyces cerevisiae)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase epsilon catalytic subunit (133 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (3 kDa, DNA from DNA molecule)
Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA complex in Apo state conformation I
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8tw7
Q-score: 0.488
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ DOI: doi:10.1126/science.adk5901 Pubmed: 39088616 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 2 (38 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 4 (35 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (30 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Proliferating cell nuclear antigen (28 kDa, Protein from Saccharomyces cerevisiae)
- Ctf18-rfc-pcna complex (Complex from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA complex in Apo state conformation I
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8tw8
Q-score: 0.426
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ DOI: doi:10.1126/science.adk5901 Pubmed: 39088616 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 2 (38 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 4 (35 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (30 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Proliferating cell nuclear antigen (28 kDa, Protein from Saccharomyces cerevisiae)
- Ctf18-rfc-pcna complex (Complex from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae PolE-Ctf18-8-1-DNA
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8tw9
Q-score: 0.438
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ DOI: doi:10.1126/science.adk5901 Pubmed: 39088616 ]
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (3 kDa, Protein from Saccharomyces cerevisiae)
- Sister chromatid cohesion protein dcc1 (44 kDa, Protein from Saccharomyces cerevisiae)
- Primer dna (2 kDa, DNA from synthetic construct)
- Template dna (4 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Chromosome transmission fidelity protein 8 (15 kDa, Protein from Saccharomyces cerevisiae)
- Ctf18-rfc-pcna complex (Complex from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA-PolE-DNA complex
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8twa
Q-score: 0.349
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ DOI: doi:10.1126/science.adk5901 Pubmed: 39088616 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 2 (38 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (3 kDa, Protein from Saccharomyces cerevisiae)
- Sister chromatid cohesion protein dcc1 (44 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 4 (35 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (84 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Primer dna (2 kDa, DNA from synthetic construct)
- Template dna (4 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Chromosome transmission fidelity protein 8 (15 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Ctf18-rfc-pcna-pole-dna complex (Complex from Saccharomyces cerevisiae)
- Proliferating cell nuclear antigen (28 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA-DNA complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8twb
Q-score: 0.482
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ DOI: doi:10.1126/science.adk5901 Pubmed: 39088616 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 2 (38 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (5 kDa, DNA from synthetic construct)
- Replication factor c subunit 4 (35 kDa, Protein from Saccharomyces cerevisiae)
- Primer dna (5 kDa, DNA from synthetic construct)
- Replication factor c subunit 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (30 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Proliferating cell nuclear antigen (28 kDa, Protein from Saccharomyces cerevisiae)
- Ctf18-rfc-pcna complex (Complex from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase in Apo state conformation I
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8foc
Q-score: 0.407
Yuan Z, Georgescu R, Li H, O'Donnell ME
Nat Commun (2023) 14 pp. 3697-3697 [ Pubmed: 37344454 DOI: doi:10.1038/s41467-023-39441-1 ]
- Dna polymerase alpha/primase complex (Complex from Saccharomyces cerevisiae)
- Dna primase (47 kDa, Protein from Saccharomyces cerevisiae)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase (167 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex in Apo state conformation II
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8fod
Q-score: 0.357
Yuan Z, Georgescu R, Li H, O'Donnell ME
Nat Commun (2023) 14 pp. 3697-3697 [ Pubmed: 37344454 DOI: doi:10.1038/s41467-023-39441-1 ]
- Dna polymerase alpha/primase complex (Complex from Saccharomyces cerevisiae)
- Dna primase (47 kDa, Protein from Saccharomyces cerevisiae)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase (167 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex bound to a template DNA
Resolution: 5.6 Å
EM Method: Single-particle
Fitted PDBs: 8foe
Q-score: 0.212
Yuan Z, Georgescu R, Li H, O'Donnell ME
Nat Commun (2023) 14 pp. 3697-3697 [ Pubmed: 37344454 DOI: doi:10.1038/s41467-023-39441-1 ]
- Dna polymerase alpha/primase complex (Complex from Saccharomyces cerevisiae)
- Dna primase (47 kDa, Protein from Saccharomyces cerevisiae)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase (167 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex in the RNA synthesis state
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 8foh
Q-score: 0.358
Yuan Z, Georgescu R, Li H, O'Donnell ME
Nat Commun (2023) 14 pp. 3697-3697 [ Pubmed: 37344454 DOI: doi:10.1038/s41467-023-39441-1 ]
- Dna polymerase alpha/primase complex (Complex from Saccharomyces cerevisiae)
- Dna primase (47 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (3 kDa, DNA from synthetic construct)
- Primer rna (2 kDa, RNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase (167 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)