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author:"Li H" AND author_name:"Zhao Y" AND database:"EMDB" AND author_name:"Oost"
CryoEM structure of ThermoCas9 in post-cleavage state bound with the DNA containing NNNNCCA PAM
Release date: Feb. 26, 2025
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 9ar4
Q-score: 0.558
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 9ar4
Q-score: 0.558
Roth M, Shu Y, Trasanidis D, Zhao Y, Hoffman R, van der Oost J, Li H
To Be Published
Sample:
- Single guide rna (47 kDa, RNA from Geobacillus thermodenitrificans)
- Cleaved 5' target dna strand (4 kDa, DNA from synthetic construct)
- Cleaved 5' non-target dna strand (1 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Thermocas9 post-cleavage complex (Complex from Geobacillus thermodenitrificans)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Cleaved 3' target dna strand (7 kDa, DNA from synthetic construct)
- Cleaved 3' non-target dna strand (4 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas9 (126 kDa, Protein from Geobacillus thermodenitrificans)
CryoEM structure of ThermoCas9 in pre-cleavage state bound with the DNA containing NNNNCCA PAM
Release date: Feb. 26, 2025
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 9ar6
Q-score: 0.501
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 9ar6
Q-score: 0.501
Roth M, Shu Y, Trasanidou D, Zhao Y, van der Oost J, Li H
To Be Published
Sample:
- Ternary complex of thermocas9 with single guide rna and target dna (Complex from Geobacillus thermodenitrificans)
- Water (18 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Target-strand dna (12 kDa, DNA from Geobacillus thermodenitrificans)
- Non-target dna (3 kDa, DNA from Geobacillus thermodenitrificans)
- Crispr-associated endonuclease cas9 (126 kDa, Protein from Geobacillus thermodenitrificans)
- Single guide rna (47 kDa, RNA from Geobacillus thermodenitrificans)
CryoEM structure of ThermoCas9 bound with target DNA strand only
Release date: Feb. 26, 2025
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 9ar7
Q-score: 0.518
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 9ar7
Q-score: 0.518
Roth M, Shu Y, Trasandidou D, Zhao Y, van der Oost J, Li H
To Be Published
Sample:
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Cleaved 5' target strand (4 kDa, DNA from Geobacillus thermodenitrificans)
- Cleaved 3' target dna strand (12 kDa, DNA from Geobacillus thermodenitrificans)
- Single guide rna (47 kDa, RNA from Geobacillus thermodenitrificans)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of thermocas9 with rna and dna (Complex from Geobacillus thermodenitrificans NG80-2)
- Crispr-associated endonuclease cas9 (126 kDa, Protein from Geobacillus thermodenitrificans)