Structure of electron bifurcating Ni-Fe hydrogenase complex HydABCSL in FMN-free apo state
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7t2r
Q-score: 0.452
Feng X, Schut GJ, Haja DK, Adams MWW, Li H
Sci Adv (2022) 8 pp. eabm7546-eabm7546 [ DOI: doi:10.1126/sciadv.abm7546 Pubmed: 35213221 ]
- Nife hydrogenase complex abcsl (500 kDa, Complex from Acetomicrobium mobile)
- Nife hydrogenase large subunit (53 kDa, Protein from Acetomicrobium mobile)
- Nife hydrogenase small subunit (19 kDa, Protein from Acetomicrobium mobile)
- Nickel (iii) ion (58 Da, Ligand)
- Carbonmonoxide-(dicyano) iron (135 Da, Ligand)
- Nife hydrogenase subunit a (76 kDa, Protein from Acetomicrobium mobile)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nife hydrogenase subunit b (65 kDa, Protein from Acetomicrobium mobile)
- Nife hydrogenase subunit c (17 kDa, Protein from Acetomicrobium mobile)
- Iron/sulfur cluster (351 Da, Ligand)
Structure of electron bifurcating Ni-Fe hydrogenase complex HydABCSL in FMN/NAD(H) bound state
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7t30
Q-score: 0.51
Feng X, Schut GJ, Haja DK, Adams MWW, Li H
Sci Adv (2022) 8 pp. eabm7546-eabm7546 [ DOI: doi:10.1126/sciadv.abm7546 Pubmed: 35213221 ]
- Nife hydrogenase complex abcsl (500 kDa, Complex from Acetomicrobium mobile)
- Nife hydrogenase large subunit (53 kDa, Protein from Acetomicrobium mobile)
- Nife hydrogenase small subunit (19 kDa, Protein from Acetomicrobium mobile)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- Nife hydrogenase subunit a (76 kDa, Protein from Acetomicrobium mobile)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nickel (iii) ion (58 Da, Ligand)
- Carbonmonoxide-(dicyano) iron (135 Da, Ligand)
- Nife hydrogenase subunit b (65 kDa, Protein from Acetomicrobium mobile)
- Nife hydrogenase subunit c (17 kDa, Protein from Acetomicrobium mobile)
- Iron/sulfur cluster (351 Da, Ligand)
Electron bifurcating flavoprotein Fix/EtfABCX
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7koe
Q-score: 0.56
Feng X, Schut GJ, Lipscomb GL, Li H, Adams MWW
PNAS (2021) 118 [ Pubmed: 33372143 DOI: doi:10.1073/pnas.2016978118 ]
- Ferredoxin-like protein (10 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099))
- Octameric complex of electron bifurcating flavoprotein fix/etfabcx (Complex from Thermotoga maritima MSB8)
- Electron transfer flavoprotein, alpha subunit (36 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099))
- Electron transfer flavoprotein-quinone oxidoreductase fixc (49 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099))
- Iron/sulfur cluster (351 Da, Ligand)
- Menaquinone-7 (648 Da, Ligand)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Electron transfer flavoprotein, beta subunit (33 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099))