Cryo-EM map of human GlcNAc-1-phosphotransferase A2B2 subcomplex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7s05
Q-score: 0.503
Li H, Lee WS, Feng X, Bai L, Jennings BC, Liu L, Doray B, Canfield WM, Kornfeld S, Li H
Nat Struct Mol Biol (2022) 29 pp. 348-356 [ Pubmed: 35332324 DOI: doi:10.1038/s41594-022-00748-0 ]
- Glcnac-1-phosphotransferase (Complex from Homo sapiens)
- N-acetylglucosamine-1-phosphotransferase subunits alpha/beta (134 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Cryo-EM structure of human GlcNAc-1-phosphotransferase A2B2 subcomplex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7s06
Q-score: 0.425
Li H, Lee WS, Feng X, Bai L, Jennings BC, Liu L, Doray B, Canfield WM, Kornfeld S, Li H
Nat Struct Mol Biol (2022) 29 pp. 348-356 [ Pubmed: 35332324 DOI: doi:10.1038/s41594-022-00748-0 ]
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7vh5
Q-score: 0.401
Zhao P, Zhao C, Chen D, Yun C, Li H, Bai L
Nat Commun (2021) 12 pp. 6439-6439 [ Pubmed: 34750373 DOI: doi:10.1038/s41467-021-26782-y ]
- Sphingosine (299 Da, Ligand)
- Plasma membrane atpase 1 (99 MDa, Complex from Saccharomyces cerevisiae)
- Plasma membrane atpase 1 (99 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7vh6
Q-score: 0.289
Zhao P, Zhao C, Chen D, Yun C, Li H, Bai L
Nat Commun (2021) 12 pp. 6439-6439 [ Pubmed: 34750373 DOI: doi:10.1038/s41467-021-26782-y ]
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- Plasma membrane atpase 1 (99 MDa, Complex from Saccharomyces cerevisiae)
- Plasma membrane atpase 1 (99 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Beryllium trifluoride ion (66 Da, Ligand)
Structure of the S. cerevisiae P4B ATPase lipid flippase in the E2P state
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 7rd6
Q-score: 0.439
Bai L, Jain BK, You Q, Duan HD, Takar M, Graham TR, Li H
Nat Commun (2021) 12 pp. 5963-5963 [ Pubmed: 34645814 DOI: doi:10.1038/s41467-021-26273-0 ]
- Beryllium trifluoride ion (66 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Probable phospholipid-transporting atpase neo1 (130 kDa, Protein from Saccharomyces cerevisiae)
- P4b atpase lipid flippase in the e2p state (Complex from Saccharomyces cerevisiae)
Structure of the S. cerevisiae P4B ATPase lipid flippase in the E2P-transition state
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 7rd7
Q-score: 0.48
Bai L, Jain BK, You Q, Duan HD, Takar M, Graham TR, Li H
Nat Commun (2021) 12 pp. 5963-5963 [ DOI: doi:10.1038/s41467-021-26273-0 Pubmed: 34645814 ]
- Tetrafluoroaluminate ion (102 Da, Ligand)
- P4b atpase lipid flippase in the e2p-transition state (Complex from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Probable phospholipid-transporting atpase neo1 (130 kDa, Protein from Saccharomyces cerevisiae)
Structure of the S. cerevisiae P4B ATPase lipid flippase in the E1-ATP state
Resolution: 5.64 Å
EM Method: Single-particle
Fitted PDBs: 7rd8
Q-score: 0.195
Bai L, Jain BK, You Q, Duan HD, Takar M, Graham TR, Li H
Nat Commun (2021) 12 pp. 5963-5963 [ DOI: doi:10.1038/s41467-021-26273-0 Pubmed: 34645814 ]
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Probable phospholipid-transporting atpase neo1 (130 kDa, Protein from Saccharomyces cerevisiae)
- P4b atpase lipid flippase in the e1-atp state (Complex from Saccharomyces cerevisiae)
Resolution: 3.98 Å
EM Method: Single-particle
Fitted PDBs: 7ky5
Q-score: 0.295
Bai L, You Q, Jain BK, Duan HD, Kovach A, Graham TR, Li H
eLife (2020) 9 [ Pubmed: 33320091 DOI: doi:10.7554/eLife.62163 ]
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phospholipid-transporting atpase dnf2 (182 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- S. cerevisiae phosphatidylcholine flippase dnf2-lem3 complex in the e2p transition state (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Structure of the S. cerevisiae phosphatidylcholine flippase Dnf1-Lem3 complex in the apo E1 state
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ky6
Q-score: 0.515
Bai L, You Q, Jain BK, Duan HD, Kovach A, Graham TR, Li H
eLife (2020) 9 [ Pubmed: 33320091 DOI: doi:10.7554/eLife.62163 ]
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- S. cerevisiae phosphatidylcholine flippase dnf1-lem3 complex in the apo e1 state (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phospholipid-transporting atpase dnf1 (178 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of the S. cerevisiae phosphatidylcholine flippase Dnf2-Lem3 complex in the apo E1 state
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 7ky7
Q-score: 0.469
Bai L, You Q, Jain BK, Duan HD, Kovach A, Graham TR, Li H
eLife (2020) 9 [ Pubmed: 33320091 DOI: doi:10.7554/eLife.62163 ]
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Phospholipid-transporting atpase dnf2 (182 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- S. cerevisiae phosphatidylcholine flippase dnf2-lem3 complex in the apo e1 state (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Cholesterol (386 Da, Ligand)