Cryo-EM map of semi-attached mutant OCCM-DNA complex (ORC-Cdc6-Cdt1-Mcm2-7 with Mcm6 WHD truncation)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6wgc
Q-score: 0.301
Yuan Z, Schneider S, Dodd T, Riera A, Bai L, Yan C, Magdalou I, Ivanov I, Stillman B, Li H, Speck C
PNAS (2020) 117 pp. 17747-17756 [ DOI: doi:10.1073/pnas.2006231117 Pubmed: 32669428 ]
- Dna (41-mer) (12 kDa, DNA from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae)
- Dna (41-mer) (12 kDa, DNA from Saccharomyces cerevisiae)
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 1 (104 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 6 (58 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 4 (60 kDa, Protein from Saccharomyces cerevisiae)
- Orc-cdc6-mcm3-mcm7-dsdna (Complex from Saccharomyces cerevisiae)
Cryo-EM map of mutant Mcm2-7 hexamer with Mcm6 WHD truncation
Resolution: 7.7 Å
EM Method: Single-particle
Fitted PDBs: 6wgf
Q-score: 0.064
Yuan Z, Schneider S, Dodd T, Riera A, Bai L, Yan C, Magdalou I, Ivanov I, Stillman B, Li H, Speck C
PNAS (2020) 117 pp. 17747-17756 [ DOI: doi:10.1073/pnas.2006231117 Pubmed: 32669428 ]
- Mcm2-7 (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM map of pre-insertion mutant OCCM-DNA complex (ORC-Cdc6-Cdt1-Mcm2-7 with Mcm6 WHD truncation)
Resolution: 8.1 Å
EM Method: Single-particle
Fitted PDBs: 6wgg
Q-score: 0.104
Yuan Z, Schneider S, Dodd T, Riera A, Bai L, Yan C, Magdalou I, Ivanov I, Stillman B, Li H, Speck C
PNAS (2020) 117 pp. 17747-17756 [ DOI: doi:10.1073/pnas.2006231117 Pubmed: 32669428 ]
- Cell division cycle protein cdt1 (68 kDa, Protein from Saccharomyces cerevisiae)
- Dna (41-mer) (12 kDa, DNA from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Orc-cdc6-mcm2-7-dsdna (Complex from Saccharomyces cerevisiae)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 1 (104 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 6 (58 kDa, Protein from Saccharomyces cerevisiae)
- Dna (41-mer) (12 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 4 (60 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 10.0 Å
EM Method: Single-particle
Fitted PDBs: 6wgi
Q-score: 0.086
Yuan Z, Schneider S, Dodd T, Riera A, Bai L, Yan C, Magdalou I, Ivanov I, Stillman B, Li H, Speck C
PNAS (2020) 117 pp. 17747-17756 [ DOI: doi:10.1073/pnas.2006231117 Pubmed: 32669428 ]
- Cell division cycle protein cdt1 (68 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 1 (104 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 6 (58 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae)
- Dna (34-mer) (10 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 4 (60 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (34-mer) (10 kDa, DNA from Saccharomyces cerevisiae)
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae)
- Orc-cdc6-mcm-cdt1-dsdna (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
Structure of the S. cerevisiae replicative helicase CMG in complex with a forked DNA
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6u0m
Q-score: 0.219
Yuan Z, Georgescu R, Bai L, Zhang D, Li H, O'Donnell ME
Nat Commun (2020) 11 pp. 688- [ DOI: doi:10.1038/s41467-020-14577-6 ]
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Dna (26-mer) (7 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (23 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (21 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (73 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (82 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (85 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 45 (73 kDa, Protein from Saccharomyces cerevisiae)
- Cmg-forkdna (Complex from Saccharomyces cerevisiae)
- Dna (15-mer) (4 kDa, DNA from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna replication licensing factor mcm2 (74 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (74 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (79 kDa, Protein from Saccharomyces cerevisiae)
Structure of Ctf4 trimer in complex with three CMG helicases
Resolution: 7.0 Å
EM Method: Single-particle
Fitted PDBs: 6pto
Q-score: 0.087
Yuan Z, Georgescu R, Santos RLA, Zhang D, Bai L, Yao NY, Zhao G, O'Donnell ME, Li H
eLife (2019) 8 [ Pubmed: 31589141 DOI: doi:10.7554/eLife.47405 ]
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 45 (74 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha-binding protein (104 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (21 kDa, Protein from Saccharomyces cerevisiae)
- Cmg-cmg-cmg-ctf4 (Complex from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
Yuan Z, Georgescu R, Santos RLA, Zhang D, Bai L, Yao NY, Zhao G, O'Donnell ME, Li H
eLife (2019) 8 [ Pubmed: 31589141 DOI: doi:10.7554/eLife.47405 ]
Cryo-EM structure of Mcm2-7 double hexamer on dsDNA
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5bk4
Q-score: 0.393
Noguchi Y, Yuan Z, Bai L, Schneider S, Zhao G, Stillman B, Speck C, Li H
PNAS (2017) 114 pp. E9529-E9538 [ Pubmed: 29078375 DOI: doi:10.1073/pnas.1712537114 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mcm double hexamer bound to double-stranded dna (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna (60-mer), strand 1 (18 kDa, DNA from Saccharomyces cerevisiae)
- Mcm double hexamer (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Dna (60-mer), strand 2 (18 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Synthetic double-stranded dna (Complex from Saccharomyces cerevisiae)
Structural basis of MCM2-7 replicative helicase loading by ORC-Cdc6 and Cdt1
Yuan Z, Riera A, Bai L, Sun J, Nandi S, Spanos C, Chen ZA, Barbon M, Rappsilber J, Stillman B, Speck C, Li H
Nat Struct Mol Biol (2017) 24 pp. 316-324 [ DOI: doi:10.1038/nsmb.3372 Pubmed: 28191893 ]
- Orc-cdc6-cdt1-mcm2-7 (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (39-mer) (11 kDa, DNA from synthetic construct)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Cell division control protein 6 (58 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (39-mer) (12 kDa, DNA from synthetic construct)
- Origin recognition complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Origin recognition complex subunit 4 (60 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Cell division cycle protein cdt1 (68 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Origin recognition complex subunit 1 (104 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Structure of Ctf4 trimer in complex with one CMG helicase
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6ptj
Q-score: 0.414
Yuan Z, Georgescu R, Santos RLA, Zhang D, Bai L, Yao NY, Zhao G, O'Donnell ME, Li H
eLife (2019) 8 [ Pubmed: 31589141 DOI: doi:10.7554/eLife.47405 ]
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 45 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Cmg-ctf4 (Complex from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha-binding protein (104 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)