Resolution: 3.98 Å
EM Method: Single-particle
Fitted PDBs: 7ky5
Q-score: 0.295
Bai L, You Q, Jain BK, Duan HD, Kovach A, Graham TR, Li H
eLife (2020) 9 [ Pubmed: 33320091 DOI: doi:10.7554/eLife.62163 ]
- S. cerevisiae phosphatidylcholine flippase dnf2-lem3 complex in the e2p transition state (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Phospholipid-transporting atpase dnf2 (182 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
Structure of the S. cerevisiae phosphatidylcholine flippase Dnf1-Lem3 complex in the apo E1 state
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ky6
Q-score: 0.515
Bai L, You Q, Jain BK, Duan HD, Kovach A, Graham TR, Li H
eLife (2020) 9 [ Pubmed: 33320091 DOI: doi:10.7554/eLife.62163 ]
- Phospholipid-transporting atpase dnf1 (178 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- S. cerevisiae phosphatidylcholine flippase dnf1-lem3 complex in the apo e1 state (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Structure of the S. cerevisiae phosphatidylcholine flippase Dnf2-Lem3 complex in the apo E1 state
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 7ky7
Q-score: 0.469
Bai L, You Q, Jain BK, Duan HD, Kovach A, Graham TR, Li H
eLife (2020) 9 [ Pubmed: 33320091 DOI: doi:10.7554/eLife.62163 ]
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phospholipid-transporting atpase dnf2 (182 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- S. cerevisiae phosphatidylcholine flippase dnf2-lem3 complex in the apo e1 state (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Cholesterol (386 Da, Ligand)
Structure of the S. cerevisiae phosphatidylcholine flippase Dnf2-Lem3 complex in the E1-ATP state
Resolution: 3.85 Å
EM Method: Single-particle
Fitted PDBs: 7ky8
Q-score: 0.452
Bai L, You Q, Jain BK, Duan HD, Kovach A, Graham TR, Li H
eLife (2020) 9 [ Pubmed: 33320091 DOI: doi:10.7554/eLife.62163 ]
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Phospholipid-transporting atpase dnf2 (182 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- S. cerevisiae phosphatidylcholine flippase dnf2-lem3 complex in the e1-atp state (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Alpha-d-mannopyranose (180 Da, Ligand)
Structure of the S. cerevisiae phosphatidylcholine flippase Dnf2-Lem3 complex in the E1-ADP state
Resolution: 4.05 Å
EM Method: Single-particle
Fitted PDBs: 7ky9
Q-score: 0.269
Bai L, You Q, Jain BK, Duan HD, Kovach A, Graham TR, Li H
eLife (2020) 9 [ Pubmed: 33320091 DOI: doi:10.7554/eLife.62163 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Alpha-d-mannopyranose (180 Da, Ligand)
- Phospholipid-transporting atpase dnf2 (182 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- S. cerevisiae phosphatidylcholine flippase dnf2-lem3 complex in the e1-adp state (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tetrafluoroaluminate ion (102 Da, Ligand)
Structure of the S. cerevisiae phosphatidylcholine flippase Dnf2-Lem3 complex in the E2P state
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7kya
Q-score: 0.473
Bai L, You Q, Jain BK, Duan HD, Kovach A, Graham TR, Li H
eLife (2020) 9 [ Pubmed: 33320091 DOI: doi:10.7554/eLife.62163 ]
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Phospholipid-transporting atpase dnf2 (182 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- S. cerevisiae phosphatidylcholine flippase dnf2-lem3 complex in the e2p state (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Structure of the S. cerevisiae phosphatidylcholine flippase Dnf1-Lem3 complex in the E1-ADP state
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7kyb
Q-score: 0.545
Bai L, You Q, Jain BK, Duan HD, Kovach A, Graham TR, Li H
eLife (2020) 9 [ Pubmed: 33320091 DOI: doi:10.7554/eLife.62163 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phospholipid-transporting atpase dnf1 (178 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Cryo-em 3d map of the s. cerevisiae phosphatidylcholine flippase dnf1-lem3 complex in the apo e1 state (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
Structure of the S. cerevisiae phosphatidylcholine flippase Dnf1-Lem3 complex in the E2P state
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7kyc
Q-score: 0.613
Bai L, You Q, Jain BK, Duan HD, Kovach A, Graham TR, Li H
eLife (2020) 9 [ Pubmed: 33320091 DOI: doi:10.7554/eLife.62163 ]
- Phospholipid-transporting atpase dnf1 (178 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Alkylphosphocholine resistance protein lem3 (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- S. cerevisiae phosphatidylcholine flippase dnf1-lem3 complex in the e2p state (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
Structure of Ctf4 trimer in complex with three CMG helicases
Resolution: 7.0 Å
EM Method: Single-particle
Fitted PDBs: 6pto
Q-score: 0.087
Yuan Z, Georgescu R, Santos RLA, Zhang D, Bai L, Yao NY, Zhao G, O'Donnell ME, Li H
eLife (2019) 8 [ Pubmed: 31589141 DOI: doi:10.7554/eLife.47405 ]
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 45 (74 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha-binding protein (104 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (21 kDa, Protein from Saccharomyces cerevisiae)
- Cmg-cmg-cmg-ctf4 (Complex from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
Yuan Z, Georgescu R, Santos RLA, Zhang D, Bai L, Yao NY, Zhao G, O'Donnell ME, Li H
eLife (2019) 8 [ Pubmed: 31589141 DOI: doi:10.7554/eLife.47405 ]