Resolution: 2.72 Å
EM Method: Single-particle
Fitted PDBs: 9dzu
Q-score: 0.479
Chen X, Duan HD, Hoy MJ, Koteva K, Spitzer M, Guitor AK, Puumala E, Hu G, Yiu B, Chou S, Bian Z, Guo ABY, Sun S, Robbins N, Cook MA, Truant R, MacNeil LT, Brown ED, Kronstad JW, Cowen LE, Heitman J, Li H, Wright GD
bioRxiv (2025) [ Pubmed: 39829750 DOI: doi:10.1101/2025.01.06.630955 ]
- Apt1-cdc50 heterodimer bound with butyrolactol a (222 kDa, Complex from Cryptococcus neoformans var. grubii H99)
- Beryllium trifluoride ion (66 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transcription regulator (45 kDa, Protein from Cryptococcus neoformans var. grubii H99)
- Phospholipid-transporting atpase (176 kDa, Protein from Cryptococcus neoformans var. grubii H99)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (3r,4r,5s)-3,4-dihydroxy-5-[(1r,2r,3s,4s,5r,6r,10e,14z,16e,18z)-1,2,3,4,5-pentahydroxy-6,20,20-trimethylhenicosa-10,14,16,18-tetraen-1-yl]oxolan-2-one (526 Da, Ligand)
Cryo-EM structure of the C. neoformans lipid flippase Apt1-Cdc50 in the E1 state
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 9dzv
Q-score: 0.399
Chen X, Duan HD, Hoy MJ, Koteva K, Spitzer M, Guitor AK, Puumala E, Hu G, Yiu B, Chou S, Bian Z, Guo ABY, Sun S, Robbins N, Cook MA, Truant R, MacNeil LT, Brown ED, Kronstad JW, Cowen LE, Heitman J, Li H, Wright GD
bioRxiv (2025) [ Pubmed: 39829750 DOI: doi:10.1101/2025.01.06.630955 ]
- Magnesium ion (24 Da, Ligand)
- Apt1-cdc50 heterodimer (222 kDa, Complex from Cryptococcus neoformans var. grubii H99)
- Transcription regulator (45 kDa, Protein from Cryptococcus neoformans var. grubii H99)
- Phospholipid-transporting atpase (176 kDa, Protein from Cryptococcus neoformans var. grubii H99)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Human TPC2 in Complex with Antagonist (S)-SG-094
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8ouo
Q-score: 0.509
Chi G, Jaslan D, Kudrina V, Bock J, Li H, Pike ACW, Rautenberg S, Krogsaeter E, Bohstedt T, Wang D, McKinley G, Fernandez-Cid A, Mukhopadhyay SMM, Burgess-Brown NA, Keller M, Bracher F, Grimm C, Durr KL
Structure (2024) 32 pp. 1137-1149.e4 [ Pubmed: 38815576 DOI: doi:10.1016/j.str.2024.05.005 ]
- 2-{[(4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25r)-spirost-5-en-3-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranoside (1 kDa, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Homodimeric complex of human two-pore channel 2 (tpc2) (150 kDa, Complex from Homo sapiens)
- Two pore channel protein 2 (85 kDa, Protein from Homo sapiens)
- 1,2-diacyl-sn-glycero-3-phoshocholine (734 Da, Ligand)
- Di-heneicosanoyl phosphatidyl choline (875 Da, Ligand)
- (1s)-6-methoxy-2-methyl-7-phenoxy-1-[(4-phenoxyphenyl)methyl]-3,4-dihydro-1h-isoquinoline (451 Da, Ligand)
Human TPC2 in Complex withAntagonist (R)-SG-094
Chi G, Jaslan D, Kudrina V, Bock J, Li H, Pike ACW, Rautenberg S, Krogsaeter E, Bohstedt T, Wang D, McKinley G, Fernandez-Cid A, Mukhopadhyay SMM, Burgess-Brown NA, Keller M, Bracher F, Grimm C, Durr KL
Structure (2024) 32 pp. 1137-1149.e4 [ Pubmed: 38815576 DOI: doi:10.1016/j.str.2024.05.005 ]