Cryo-EM structure of S. cerevisiae PolE-Ctf18-8-1-DNA
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8tw9
Q-score: 0.438
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ DOI: doi:10.1126/science.adk5901 Pubmed: 39088616 ]
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (3 kDa, Protein from Saccharomyces cerevisiae)
- Sister chromatid cohesion protein dcc1 (44 kDa, Protein from Saccharomyces cerevisiae)
- Primer dna (2 kDa, DNA from synthetic construct)
- Template dna (4 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Chromosome transmission fidelity protein 8 (15 kDa, Protein from Saccharomyces cerevisiae)
- Ctf18-rfc-pcna complex (Complex from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA-PolE-DNA complex
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8twa
Q-score: 0.349
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ DOI: doi:10.1126/science.adk5901 Pubmed: 39088616 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 2 (38 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (3 kDa, Protein from Saccharomyces cerevisiae)
- Sister chromatid cohesion protein dcc1 (44 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 4 (35 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (84 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Primer dna (2 kDa, DNA from synthetic construct)
- Template dna (4 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Chromosome transmission fidelity protein 8 (15 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Ctf18-rfc-pcna-pole-dna complex (Complex from Saccharomyces cerevisiae)
- Proliferating cell nuclear antigen (28 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA-DNA complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8twb
Q-score: 0.482
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ DOI: doi:10.1126/science.adk5901 Pubmed: 39088616 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 2 (38 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (5 kDa, DNA from synthetic construct)
- Replication factor c subunit 4 (35 kDa, Protein from Saccharomyces cerevisiae)
- Primer dna (5 kDa, DNA from synthetic construct)
- Replication factor c subunit 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (30 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Proliferating cell nuclear antigen (28 kDa, Protein from Saccharomyces cerevisiae)
- Ctf18-rfc-pcna complex (Complex from Saccharomyces cerevisiae)
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 8umv
Q-score: 0.533
He Q, Wang F, O'Donnell ME, Li H
PNAS (2024) 121 pp. e2319727121-e2319727121 [ Pubmed: 38669181 DOI: doi:10.1073/pnas.2319727121 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- The human clamp-clamp loader complex pcna-ctf18 (300 kDa, Complex from Homo sapiens)
- Replication factor c subunit 3 (40 kDa, Protein from Homo sapiens)
- Proliferating cell nuclear antigen (28 kDa, Protein from Homo sapiens)
- Chromosome transmission fidelity protein 18 homolog (107 kDa, Protein from Homo sapiens)
- Dna (40-mer) (12 kDa, DNA from synthetic construct)
- Replication factor c subunit 4 (39 kDa, Protein from Homo sapiens)
- Replication factor c subunit 5 (38 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Homo sapiens)
- Dna (20-mer) (6 kDa, DNA from synthetic construct)
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8umw
Q-score: 0.495
He Q, Wang F, O'Donnell ME, Li H
PNAS (2024) 121 pp. e2319727121-e2319727121 [ Pubmed: 38669181 DOI: doi:10.1073/pnas.2319727121 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- The human clamp-clamp loader complex pcna-ctf18 (300 kDa, Complex from Homo sapiens)
- Replication factor c subunit 3 (40 kDa, Protein from Homo sapiens)
- Proliferating cell nuclear antigen (28 kDa, Protein from Homo sapiens)
- Chromosome transmission fidelity protein 18 homolog (107 kDa, Protein from Homo sapiens)
- Dna (40-mer) (12 kDa, DNA from synthetic construct)
- Replication factor c subunit 4 (39 kDa, Protein from Homo sapiens)
- Replication factor c subunit 5 (38 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Homo sapiens)
- Dna (20-mer) (6 kDa, DNA from synthetic construct)
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 8umy
Q-score: 0.51
He Q, Wang F, O'Donnell ME, Li H
PNAS (2024) 121 pp. e2319727121-e2319727121 [ Pubmed: 38669181 DOI: doi:10.1073/pnas.2319727121 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- The human clamp-clamp loader complex pcna-ctf18 (300 kDa, Complex from Homo sapiens)
- Replication factor c subunit 3 (40 kDa, Protein from Homo sapiens)
- Proliferating cell nuclear antigen (28 kDa, Protein from Homo sapiens)
- Chromosome transmission fidelity protein 18 homolog (107 kDa, Protein from Homo sapiens)
- Dna (40-mer) (12 kDa, DNA from synthetic construct)
- Replication factor c subunit 4 (39 kDa, Protein from Homo sapiens)
- Replication factor c subunit 5 (38 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Homo sapiens)
- Dna (20-mer) (6 kDa, DNA from synthetic construct)
Cryo-EM map of the human CTF18-RFC-PCNA-DNA ternary complex with cracked and closed PCNA (state 7)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8un0
Q-score: 0.451
He Q, Wang F, O'Donnell ME, Li H
PNAS (2024) 121 pp. e2319727121-e2319727121 [ Pubmed: 38669181 DOI: doi:10.1073/pnas.2319727121 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- The human clamp-clamp loader complex pcna-ctf18 (300 kDa, Complex from Homo sapiens)
- Replication factor c subunit 3 (40 kDa, Protein from Homo sapiens)
- Proliferating cell nuclear antigen (28 kDa, Protein from Homo sapiens)
- Chromosome transmission fidelity protein 18 homolog (107 kDa, Protein from Homo sapiens)
- Dna (40-mer) (12 kDa, DNA from synthetic construct)
- Replication factor c subunit 4 (39 kDa, Protein from Homo sapiens)
- Replication factor c subunit 5 (38 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Homo sapiens)
- Dna (20-mer) (6 kDa, DNA from synthetic construct)
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8fs3
Q-score: 0.503
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Checkpoint protein rad24 (62 kDa, Protein from Saccharomyces cerevisiae)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8fs4
Q-score: 0.452
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand 1 (6 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Checkpoint protein rad24 (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8fs5
Q-score: 0.548
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Checkpoint protein rad24 (62 kDa, Protein from Saccharomyces cerevisiae)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Primer strand 1 (6 kDa, DNA from synthetic construct)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)