Cryo-EM structure of S. cerevisiae PolE-Ctf18-8-1-DNA
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8tw9
Q-score: 0.438
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ DOI: doi:10.1126/science.adk5901 Pubmed: 39088616 ]
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (3 kDa, Protein from Saccharomyces cerevisiae)
- Sister chromatid cohesion protein dcc1 (44 kDa, Protein from Saccharomyces cerevisiae)
- Primer dna (2 kDa, DNA from synthetic construct)
- Template dna (4 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Chromosome transmission fidelity protein 8 (15 kDa, Protein from Saccharomyces cerevisiae)
- Ctf18-rfc-pcna complex (Complex from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA-PolE-DNA complex
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8twa
Q-score: 0.349
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ DOI: doi:10.1126/science.adk5901 Pubmed: 39088616 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 2 (38 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (3 kDa, Protein from Saccharomyces cerevisiae)
- Sister chromatid cohesion protein dcc1 (44 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 4 (35 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (84 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Primer dna (2 kDa, DNA from synthetic construct)
- Template dna (4 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Chromosome transmission fidelity protein 8 (15 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Ctf18-rfc-pcna-pole-dna complex (Complex from Saccharomyces cerevisiae)
- Proliferating cell nuclear antigen (28 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA-DNA complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8twb
Q-score: 0.482
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ DOI: doi:10.1126/science.adk5901 Pubmed: 39088616 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 2 (38 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (5 kDa, DNA from synthetic construct)
- Replication factor c subunit 4 (35 kDa, Protein from Saccharomyces cerevisiae)
- Primer dna (5 kDa, DNA from synthetic construct)
- Replication factor c subunit 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (30 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Proliferating cell nuclear antigen (28 kDa, Protein from Saccharomyces cerevisiae)
- Ctf18-rfc-pcna complex (Complex from Saccharomyces cerevisiae)
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8fs3
Q-score: 0.503
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Checkpoint protein rad24 (62 kDa, Protein from Saccharomyces cerevisiae)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8fs4
Q-score: 0.452
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand 1 (6 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Checkpoint protein rad24 (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8fs5
Q-score: 0.548
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Checkpoint protein rad24 (62 kDa, Protein from Saccharomyces cerevisiae)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Primer strand 1 (6 kDa, DNA from synthetic construct)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8fs6
Q-score: 0.487
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Checkpoint protein rad24 (62 kDa, Protein from Saccharomyces cerevisiae)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Primer strand 1 (6 kDa, DNA from synthetic construct)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 8fs7
Q-score: 0.542
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Checkpoint protein rad24 (62 kDa, Protein from Saccharomyces cerevisiae)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Primer strand 1 (6 kDa, DNA from synthetic construct)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 8fs8
Q-score: 0.446
Zheng F, Georgescu RE, Yao NY, O'Donnell ME, Li H
bioRxiv (2023) [ DOI: doi:10.1101/2023.05.03.539266 Pubmed: 37205533 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand 1 (6 kDa, DNA from synthetic construct)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Checkpoint protein rad24 (57 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (15 kDa, DNA from synthetic construct)
- Rad24-rfc-911 clamp-dna (Complex)
- Primer strand 2 (6 kDa, DNA from synthetic construct)
- Ddc1 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae)
- Dna damage checkpoint control protein rad17 (45 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from synthetic construct)
- Dna damage checkpoint control protein mec3 (53 kDa, Protein from Saccharomyces cerevisiae)
- Proteins complex (Complex from Saccharomyces cerevisiae)
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7tfh
Q-score: 0.469
Zheng F, Georgescu R, Yao NY, Li H, O'Donnell ME
eLife (2022) 11 [ DOI: doi:10.7554/eLife.77469 Pubmed: 35829698 ]
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Dsdna (Complex from synthetic construct)
- Proliferating cell nuclear antigen (29 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand (6 kDa, DNA from synthetic construct)
- Rfc-pcna-dna1-dna2 (Complex)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (12 kDa, DNA from synthetic construct)
- Replication factor c subunit 1 (95 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Proteins (Complex from Saccharomyces cerevisiae)