Cryo-EM structure of SNAP-94847-bound MCHR1, S1 state
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 8yns
Q-score: 0.476
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Antibody 1b3 fab light chain (23 kDa, Protein from synthetic construct)
- Snap-94847-mchr1-fab1b3-glue complex (Complex from Homo sapiens)
- Melanin-concentrating hormone receptor 1,soluble cytochrome b562 (64 kDa, Protein from Homo sapiens)
- Glue molecule 4-9 (30 kDa, Protein from synthetic construct)
- Antibody 1b3 fab heavy chain (25 kDa, Protein from synthetic construct)
- ~{n}-[3-[1-[[4-[3,4-bis(fluoranyl)phenoxy]phenyl]methyl]piperidin-4-yl]-4-methyl-phenyl]-2-methyl-propanamide (478 Da, Ligand)
Cryo-EM structure of SNAP-94847-bound MCHR1, S2 state
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 8ynt
Q-score: 0.422
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Antibody 1b3 fab light chain (23 kDa, Protein from synthetic construct)
- Snap-94847-mchr1-fab1b3-glue complex (Complex from Homo sapiens)
- Melanin-concentrating hormone receptor 1,soluble cytochrome b562 (64 kDa, Protein from Homo sapiens)
- Glue molecule 4-9 (30 kDa, Protein from synthetic construct)
- Antibody 1b3 fab heavy chain (25 kDa, Protein from synthetic construct)
- ~{n}-[3-[1-[[4-[3,4-bis(fluoranyl)phenoxy]phenyl]methyl]piperidin-4-yl]-4-methyl-phenyl]-2-methyl-propanamide (478 Da, Ligand)
MCH-MCHR1-Gi complex, T1 state
Resolution: 2.61 Å
EM Method: Single-particle
Fitted PDBs: 8wwk
Q-score: 0.611
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mch-mchr1-gi complex t1 state (Complex from Homo sapiens)
- Melanin-concentrating hormone (2 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Fusion protein 1,melanin-concentrating hormone receptor 1,fusion protein 2 (68 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
MCH-MCHR1-Gi complex, T2 state
Resolution: 2.78 Å
EM Method: Single-particle
Fitted PDBs: 8wwl
Q-score: 0.573
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Melanin-concentrating hormone (2 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Fusion protein 1,melanin-concentrating hormone receptor 1,fusion protein 2 (68 kDa, Protein from Homo sapiens)
- Mch-mchr1-gi complex t2 state (Complex from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
MCH-MCHR1-Gi complex, L2 state
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8wwm
Q-score: 0.531
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mch-mchr1-gi complex l2 state (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Melanin-concentrating hormone (2 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Fusion protein 1,melanin-concentrating hormone receptor 1,fusion protein 2 (68 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 8wwn
Q-score: 0.6
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mch-mchr1-gi complex l1 state (Complex from Homo sapiens)
- Melanin-concentrating hormone (2 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Fusion protein 1,melanin-concentrating hormone receptor 1,fusion protein 2 (68 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
Lysophosphatidylserine receptor GPR34-Gi complex
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8iz4
Q-score: 0.52
Gong W, Liu G, Li X, Wang Y, Zhang X
To Be Published
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
- [(2~{r})-2-oxidanyl-3-phosphonooxy-propyl] octadecanoate (438 Da, Ligand)
- Serine (105 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Probable g-protein coupled receptor 34 (64 kDa, Protein from Homo sapiens)
- Lysops-gpr34-gi-scfv16 complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Lysophosphatidylserine receptor GPR34-Gi complex
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8wrb
Q-score: 0.545
Liu G, Li X, Wang Y, Zhang X, Gong W
PLoS Biol (2023) 21 pp. e3002387-e3002387 [ DOI: doi:10.1371/journal.pbio.3002387 Pubmed: 38048360 ]
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
- Serine (105 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- [(2r)-2-oxidanyl-3-phosphonooxy-propyl] (z)-octadec-9-enoate (436 Da, Ligand)
- Lysops-gpr34-gi-scfv16 complex (Complex from Homo sapiens)
- Probable g-protein coupled receptor 34,tag (64 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of an orphan GPCR-Gi protein signaling complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8g05
Q-score: 0.524
Zhang X, Wang Y, Supekar S, Cao X, Zhou J, Dang J, Chen S, Jenkins L, Marsango S, Li X, Liu G, Milligan G, Feng M, Fan H, Gong W, Zhang C
Nat Commun (2023) 14 pp. 5706-5706 [ Pubmed: 37709767 DOI: doi:10.1038/s41467-023-41201-0 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 6-(octylamino)pyrimidine-2,4(3h,5h)-dione (239 Da, Ligand)
- 6oau_gpr84_dngi_scfv16 (Complex from Spodoptera frugiperda)
- G-protein coupled receptor 84 (48 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Lysophosphatidylserine receptor GPR174-Gs complex
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 8izb
Q-score: 0.51
Liu G, Li X, Wang Y, Zhang X, Gong W
PLoS Biol (2023) 21 pp. e3002387-e3002387 [ DOI: doi:10.1371/journal.pbio.3002387 Pubmed: 38048360 ]
- Serine (105 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Lysops-bound gpr174 in complex with gs and nb35. (Complex from Homo sapiens)
- [(2r)-2-oxidanyl-3-phosphonooxy-propyl] (z)-octadec-9-enoate (436 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Probable g-protein coupled receptor 174 (63 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from synthetic construct)