Cryo-EM structure of the NmeCas9-sgRNA-AcrIIC4 ternary complex
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 8ja0
Q-score: 0.442
Li X, Liao F, Gao J, Song G, Zhang C, Ji N, Wang X, Wen J, He J, Wei Y, Zhang H, Li Z, Yu G, Yin H
Nucleic Acids Res (2023) 51 pp. 9442-9451 [ DOI: doi:10.1093/nar/gkad669 Pubmed: 37587688 ]
- Rna (117-mer) (37 kDa, RNA from Neisseria meningitidis)
- A protein complex (Complex from Neisseria meningitidis)
- Crispr-associated endonuclease cas9 (124 kDa, Protein from Neisseria meningitidis)
- Uncharacterized protein (9 kDa, Protein from Haemophilus parainfluenzae)
Cryo-EM structure of TIR-APAZ/Ago-gRNA-DNA complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8i87
Q-score: 0.53
Wang X, Li X, Yu G, Zhang L, Zhang C, Wang Y, Liao F, Wen Y, Yin H, Liu X, Wei Y, Li Z, Deng Z, Zhang H
Cell Res (2023) 33 pp. 699-711 [ DOI: doi:10.1038/s41422-023-00839-7 Pubmed: 37311833 ]
- Tir-apaz/ago-grna-dna complex (Complex from Maribacter polysiphoniae)
- Rna (5'-r(p*up*gp*ap*gp*gp*up*ap*gp*up*ap*gp*gp*up*up*gp*up*ap*up*a)-3') (6 kDa, RNA from Maribacter polysiphoniae)
- Dna (5'-d(p*tp*ap*tp*ap*cp*ap*ap*cp*cp*tp*ap*cp*tp*ap*cp*cp*tp*cp*a)-3') (5 kDa, DNA from Maribacter polysiphoniae)
- Magnesium ion (24 Da, Ligand)
- Piwi domain-containing protein (58 kDa, Protein from Maribacter polysiphoniae)
- Tir domain-containing protein (53 kDa, Protein from Maribacter polysiphoniae)
Cryo-EM structure of TIR-APAZ/Ago-gRNA complex
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8i88
Q-score: 0.433
Wang X, Li X, Yu G, Zhang L, Zhang C, Wang Y, Liao F, Wen Y, Yin H, Liu X, Wei Y, Li Z, Deng Z, Zhang H
Cell Res (2023) 33 pp. 699-711 [ DOI: doi:10.1038/s41422-023-00839-7 Pubmed: 37311833 ]
- Cryo-em structure of tir-apaz/ago-grna complex (Complex from Maribacter polysiphoniae)
- Piwi domain-containing protein (58 kDa, Protein from Maribacter polysiphoniae)
- Rna (5'-r(p*gp*a)-3') (629 Da, RNA from Maribacter polysiphoniae)
- Tir domain-containing protein (53 kDa, Protein from Maribacter polysiphoniae)
Cryo-EM structure of the target ssDNA-bound SIR2-APAZ/Ago-gRNA quaternary complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8in8
Q-score: 0.504
Wang X, Li X, Yu G, Zhang L, Zhang C, Wang Y, Liao F, Wen Y, Yin H, Liu X, Wei Y, Li Z, Deng Z, Zhang H
Cell Res (2023) 33 pp. 699-711 [ DOI: doi:10.1038/s41422-023-00839-7 Pubmed: 37311833 ]
- Magnesium ion (24 Da, Ligand)
- Dna (5'-d(p*ap*ap*cp*gp*ap*cp*gp*tp*cp*tp*ap*ap*gp*ap*ap*ap*cp*cp*ap*tp*tp*ap*a)-3') (7 kDa, DNA from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA))
- Rna (5'-r(p*ap*up*ap*ap*up*gp*gp*up*up*up*cp*up*up*ap*gp*ap*cp*gp*up*cp*gp*up*up*u)-3') (7 kDa, RNA from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA))
- Sir2 superfamily protein (66 kDa, Protein from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA))
- Cryo-em structure of the target ssdna-bound sir2-apaz/ago-grna quaternary complex (Complex from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA))
- Piwi domain protein (53 kDa, Protein from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA))
Structure of the SbCas7-11-crRNA-NTR complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8gna
Q-score: 0.544
Wang X, Yu G, Wen Y, An Q, Li X, Liao F, Lian C, Zhang K, Yin H, Wei Y, Deng Z, Zhang H
Nucleic Acids Res (2022) 50 pp. 12913-12923 [ DOI: doi:10.1093/nar/gkac1151 Pubmed: 36484100 ]
- Rna (32-mer) (10 kDa, RNA from Candidatus Scalindua brodae)
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(p*gp*gp*gp*gp*cp*ap*gp*ap*ap*ap*ap*up*up*gp*gp*gp*u)-3') (14 kDa, RNA from Candidatus Scalindua brodae)
- Ternary complex (Complex from Candidatus Scalindua brodae)
- Ramp superfamily protein (197 kDa, Protein from Candidatus Scalindua brodae)
Structure of the SbCas7-11-crRNA-NTR-Csx29 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8gu6
Q-score: 0.496
Wang X, Yu G, Wen Y, An Q, Li X, Liao F, Lian C, Zhang K, Yin H, Wei Y, Deng Z, Zhang H
Nucleic Acids Res (2022) 50 pp. 12913-12923 [ DOI: doi:10.1093/nar/gkac1151 Pubmed: 36484100 ]
- Rna (5'-r(p*gp*gp*gp*gp*cp*ap*gp*ap*ap*ap*ap*up*up*gp*gp*gp*u)-3') (5 kDa, RNA from Candidatus Scalindua brodae)
- Rna (33-mer) (10 kDa, RNA from Candidatus Scalindua brodae)
- Chat domain protein (82 kDa, Protein from Candidatus Scalindua brodae)
- Ramp superfamily protein (197 kDa, Protein from Candidatus Scalindua brodae)
- Zinc ion (65 Da, Ligand)
- Ternary complex (Complex from Candidatus Scalindua brodae)
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 7x10
Q-score: 0.465
Qian Y, Ma Z, Liu C, Li X, Zhu X, Wang N, Xu Z, Xia R, Liang J, Duan Y, Yin H, Xiong Y, Zhang A, Guo C, Chen Z, Huang Z, He Y
Mol Cell (2022) 82 pp. 4340-4352.e6 [ Pubmed: 36309016 DOI: doi:10.1016/j.molcel.2022.10.009 ]
- Isoform 3 of adhesion g protein-coupled receptor l3 (172 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Engineered g alpha 12 subunit (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (Complex from Homo sapiens)
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 7wy5
Q-score: 0.514
Qian Y, Ma Z, Liu C, Li X, Zhu X, Wang N, Xu Z, Xia R, Liang J, Duan Y, Yin H, Xiong Y, Zhang A, Guo C, Chen Z, Huang Z, He Y
Mol Cell (2022) 82 pp. 4340-4352.e6 [ Pubmed: 36309016 DOI: doi:10.1016/j.molcel.2022.10.009 ]
- Isoform 3 of adhesion g protein-coupled receptor l3 (172 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Engineered mini g alpha q subunit (41 kDa, Protein from Homo sapiens)
- Nb35 (17 kDa, Protein from Homo sapiens)
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 7wy8
Q-score: 0.504
Qian Y, Ma Z, Liu C, Li X, Zhu X, Wang N, Xu Z, Xia R, Liang J, Duan Y, Yin H, Xiong Y, Zhang A, Guo C, Chen Z, Huang Z, He Y
Mol Cell (2022) 82 pp. 4340-4352.e6 [ Pubmed: 36309016 DOI: doi:10.1016/j.molcel.2022.10.009 ]
- Nb35 (17 kDa, Protein from Lama glama)
- Isoform 3 of adhesion g protein-coupled receptor l3 (172 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nb35 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gpcr/g-protein (Complex from Lama glama)
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Engineered mini g alpha s subunit (41 kDa, Protein from Homo sapiens)
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 7wyb
Q-score: 0.352
Qian Y, Ma Z, Liu C, Li X, Zhu X, Wang N, Xu Z, Xia R, Liang J, Duan Y, Yin H, Xiong Y, Zhang A, Guo C, Chen Z, Huang Z, He Y
Mol Cell (2022) 82 pp. 4340-4352.e6 [ Pubmed: 36309016 DOI: doi:10.1016/j.molcel.2022.10.009 ]
- Gpcr/g-protein complex (Complex)
- Isoform 3 of adhesion g protein-coupled receptor l3 (172 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Adgrl3 (Complex from Mus musculus)