Cryo-EM reveals cholesterol binding in the lysosomal GPCR-like protein LYCHOS
Zhao J, Shen Q, Yong X, Li X, Tian X, Sun S, Xu Z, Zhang X, Zhang L, Yang H, Shao Z, Xu H, Jiang Y, Zhang Y, Yan W
Nat Struct Mol Biol (2025) [ Pubmed: 39824976 DOI: doi:10.1038/s41594-024-01470-9 ]
- Water (18 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Lysosomal cholesterol signaling protein (93 kDa, Protein from Homo sapiens)
- Cholesterol-bound lychos (Complex from Homo sapiens)
- [(2r)-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] octadecanoate (720 Da, Ligand)
- Cholesterol (386 Da, Ligand)
Cryo-EM Structure of MILI(K852A)-piRNA-target (26-nt)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 9ij4
Q-score: 0.474
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ Pubmed: 39814893 DOI: doi:10.1038/s41586-024-08438-1 ]
- Mili(k852a)-pirna-target (26-nt) (Complex from Homo sapiens)
- Rna (26-mer) (8 kDa, RNA from Homo sapiens)
- Piwi-like protein 2 (109 kDa, Protein from Mus musculus)
- Magnesium ion (24 Da, Ligand)
- Rna (25-mer) (8 kDa, RNA from Homo sapiens)
Cryo-EM Structure of EfPiwi-piRNA-target (25-nt, bilobed)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9iiy
Q-score: 0.315
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ Pubmed: 39814893 DOI: doi:10.1038/s41586-024-08438-1 ]
- Rna (5'-r(p*cp*gp*up*cp*up*ap*up*ap*cp*ap*ap*cp*cp*gp*ap*up*cp*ap*gp*cp*u)-3') (6 kDa, RNA from Homo sapiens)
- Piwi (110 kDa, Protein from Ephydatia fluviatilis)
- Efpiwi-pirna-target (25-nt, bilobed) (Complex from Homo sapiens)
- Rna (5'-r(p*up*ap*gp*cp*ap*gp*ap*up*cp*gp*gp*up*up*gp*up*ap*up*ap*gp*ap*cp*g)-3') (7 kDa, RNA from Homo sapiens)
Cryo-EM Structure of EfPiwi-piRNA-target (25-nt, comma)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 9iiz
Q-score: 0.214
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ Pubmed: 39814893 DOI: doi:10.1038/s41586-024-08438-1 ]
- Efpiwi-pirna-target (25-nt, comma) (Complex from Homo sapiens)
- Rna (25-mer) (7 kDa, RNA from Homo sapiens)
- Piwi (110 kDa, Protein from Ephydatia fluviatilis)
- Rna (5'-r(p*ap*gp*cp*cp*ap*ap*gp*up*up*up*cp*cp*ap*up*gp*up*up*gp*ap*up*gp*gp*up*a)-3') (7 kDa, RNA from Homo sapiens)
Cryo-EM Structure of MILI-piRNA-target (8-nt)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9ij0
Q-score: 0.417
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ Pubmed: 39814893 DOI: doi:10.1038/s41586-024-08438-1 ]
- Rna (5'-r(p*gp*ap*up*gp*gp*up*ap*a)-3') (2 kDa, RNA from Homo sapiens)
- Rna (26-mer) (8 kDa, RNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Piwi-like protein 2 (109 kDa, Protein from Mus musculus)
- Mili-pirna-target (8-nt) (Complex from Homo sapiens)
Cryo-EM Structure of MILI-piRNA-target (22-nt, bilobed)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9ij1
Q-score: 0.325
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ Pubmed: 39814893 DOI: doi:10.1038/s41586-024-08438-1 ]
- Rna (5'-r(p*cp*ap*ap*gp*up*up*up*cp*cp*ap*up*gp*up*up*gp*ap*up*gp*gp*up*a)-3') (6 kDa, RNA from Homo sapiens)
- Rna (26-mer) (8 kDa, RNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Piwi-like protein 2 (109 kDa, Protein from Mus musculus)
- Mili-pirna-target (22-nt, bilobed) (Complex from Homo sapiens)
Cryo-EM Structure of MILI-piRNA-target (22-nt, comma)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 9ij2
Q-score: 0.188
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ Pubmed: 39814893 DOI: doi:10.1038/s41586-024-08438-1 ]
- Rna (5'-r(p*up*up*ap*cp*cp*ap*up*cp*ap*ap*cp*ap*up*gp*gp*ap*ap*ap*cp*up*up*g)-3') (6 kDa, RNA from Homo sapiens)
- Piwi-like protein 2 (109 kDa, Protein from Mus musculus)
- Rna (5'-r(p*cp*ap*ap*gp*up*up*up*cp*cp*ap*up*gp*up*up*gp*ap*up*gp*gp*up*a)-3') (6 kDa, RNA from Homo sapiens)
- Mili-pirna-target (22-nt, comma) (Complex from Homo sapiens)
Cryo-EM Structure of MILI-piRNA-target (26-nt)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 9ij3
Q-score: 0.463
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ Pubmed: 39814893 DOI: doi:10.1038/s41586-024-08438-1 ]
- Rna (26-mer) (8 kDa, RNA from Homo sapiens)
- Mili-pirna-target (26-nt) (Complex from Homo sapiens)
- Rna (25-mer) (8 kDa, RNA from Homo sapiens)
- Manganese (ii) ion (54 Da, Ligand)
- Piwi-like protein 2 (109 kDa, Protein from Mus musculus)
Cryo-EM Structure of MILI(K853A)-piRNA-target
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 9ij5
Q-score: 0.461
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ Pubmed: 39814893 DOI: doi:10.1038/s41586-024-08438-1 ]
- Mili(k853a)-pirna-target (Complex from Homo sapiens)
- Rna (26-mer) (8 kDa, RNA from Homo sapiens)
- Piwi-like protein 2 (109 kDa, Protein from Mus musculus)
- Magnesium ion (24 Da, Ligand)
- Rna (25-mer) (8 kDa, RNA from Homo sapiens)
Cryo-EM structure of ETBR bound with BQ3020
Hou J, Liu S, Zhang X, Tu G, Wu L, Zhang Y, Yang H, Li X, Liu J, Jiang L, Tan Q, Bai F, Liu Z, Miao C, Hua T, Luo Z
Cell Discov (2024) 10 pp. 79-79 [ DOI: doi:10.1038/s41421-024-00705-9 Pubmed: 39075075 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Exo-alpha-sialidase,endothelin receptor type b (94 kDa, Protein from Homo sapiens)
- Complex of protein gsq with etb and bq3020 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Bq3020 (1 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from Lama glama)