Cryo-EM structure of human Spns1
Chen H, Ha HTT, Elghobashi-Meinhardt N, Le NA, Schmiege P, Nguyen LN, Li X
PNAS (2025) 122 pp. e2409596121-e2409596121 [ DOI: doi:10.1073/pnas.2409596121 Pubmed: 39739806 ]
Cryo-EM structure of human Spns1 in complex with LPC (18:1)
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 9boi
Q-score: 0.494
Chen H, Ha HTT, Elghobashi-Meinhardt N, Le NA, Schmiege P, Nguyen LN, Li X
PNAS (2025) 122 pp. e2409596121-e2409596121 [ DOI: doi:10.1073/pnas.2409596121 Pubmed: 39739806 ]
- Human spns1 purified in the presence of extra lpc addition (Cellular component from Homo sapiens)
- Mvenus,maltose/maltodextrin-binding periplasmic protein,protein spinster homolog 1,designed ankyrin repeat protein (darpin) (139 kDa, Protein from Homo sapiens)
- (4r,7r,18z)-4,7-dihydroxy-n,n,n-trimethyl-10-oxo-3,5,9-trioxa-4-phosphaheptacos-18-en-1-aminium 4-oxide (522 Da, Ligand)
Structure of Apo Sialin at pH7.5
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 8u3d
Q-score: 0.582
Schmiege P, Donnelly L, Elghobashi-Meinhardt N, Lee CH, Li X
Nat Commun (2024) 15 pp. 4386-4386 [ DOI: doi:10.1038/s41467-024-48535-3 Pubmed: 38782953 ]
- Sialin (55 kDa, Protein from Homo sapiens)
- Structure of apo sialin at ph7.5 (Complex from Homo sapiens)
Structure of Apo Sialin at pH5.0
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 8u3e
Q-score: 0.513
Schmiege P, Donnelly L, Elghobashi-Meinhardt N, Lee CH, Li X
Nat Commun (2024) 15 pp. 4386-4386 [ DOI: doi:10.1038/s41467-024-48535-3 Pubmed: 38782953 ]
- Sialin (55 kDa, Protein from Homo sapiens)
- Structure of apo sialin at ph5.0 (Complex from Homo sapiens)
Structure of Apo Sialin R168K mutant
Resolution: 3.31 Å
EM Method: Single-particle
Fitted PDBs: 8u3f
Q-score: 0.531
Schmiege P, Donnelly L, Elghobashi-Meinhardt N, Lee CH, Li X
Nat Commun (2024) 15 pp. 4386-4386 [ DOI: doi:10.1038/s41467-024-48535-3 Pubmed: 38782953 ]
- Structure of apo sialin r168k mutant (Complex from Homo sapiens)
- Sialin (55 kDa, Protein from Homo sapiens)
Structure of NAAG-bound Sialin
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 8u3g
Q-score: 0.479
Schmiege P, Donnelly L, Elghobashi-Meinhardt N, Lee CH, Li X
Nat Commun (2024) 15 pp. 4386-4386 [ DOI: doi:10.1038/s41467-024-48535-3 Pubmed: 38782953 ]
- Aspartic acid (133 Da, Ligand)
- Acetyl group (44 Da, Ligand)
- Sialin (55 kDa, Protein from Homo sapiens)
- Structure of naag-bound sialin (Complex from Homo sapiens)
- Glutamic acid (147 Da, Ligand)
Structure of Fmoc-Leu-OH bound Sialin
Resolution: 3.67 Å
EM Method: Single-particle
Fitted PDBs: 8u3h
Q-score: 0.431
Schmiege P, Donnelly L, Elghobashi-Meinhardt N, Lee CH, Li X
Nat Commun (2024) 15 pp. 4386-4386 [ DOI: doi:10.1038/s41467-024-48535-3 Pubmed: 38782953 ]
- Sialin (55 kDa, Protein from Homo sapiens)
- Structure of fmoc-leu-oh bound sialin (Complex from Homo sapiens)
- Leucine (131 Da, Ligand)
- Fluorenylmethyloxycarbonyl chloride (258 Da, Ligand)
Structure of Apo Sialin S61A mutant
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 9ayb
Q-score: 0.519
Schmiege P, Donnelly L, Elghobashi-Meinhardt N, Lee CH, Li X
Nat Commun (2024) 15 pp. 4386-4386 [ DOI: doi:10.1038/s41467-024-48535-3 Pubmed: 38782953 ]
- Structure of apo sialin s61a mutant (Complex from Homo sapiens)
- Sialin (55 kDa, Protein from Homo sapiens)
Structure of human Wnt7a bound to WLS and CALR
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8tzo
Q-score: 0.494
Qi X, Hu Q, Elghobashi-Meinhardt N, Long T, Chen H, Li X
Cell (2023) 186 pp. 5028-5040.e14 [ DOI: doi:10.1016/j.cell.2023.09.021 Pubmed: 37852257 ]
- Calreticulin (48 kDa, Protein from Homo sapiens)
- Wnt7a-wls-calr complex (Complex from Homo sapiens)
- Protein wntless homolog (62 kDa, Protein from Homo sapiens)
- Palmitoleic acid (254 Da, Ligand)
- Protein wnt-7a (39 kDa, Protein from Homo sapiens)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Structure of human Wnt7a bound to WLS and RECK
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 8tzp
Q-score: 0.481
Qi X, Hu Q, Elghobashi-Meinhardt N, Long T, Chen H, Li X
Cell (2023) 186 pp. 5028-5040.e14 [ DOI: doi:10.1016/j.cell.2023.09.021 Pubmed: 37852257 ]
- Protein wntless homolog (62 kDa, Protein from Homo sapiens)
- Palmitoleic acid (254 Da, Ligand)
- Protein wnt-7a (39 kDa, Protein from Homo sapiens)
- Reversion-inducing cysteine-rich protein with kazal motifs (106 kDa, Protein from Homo sapiens)
- Wnt7a-wls-reck complex (Complex from Homo sapiens)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)