Cryo-EM structure of the BA1-bound BRS3-Gq complex
Li C, Xu Y, Su W, He X, Li J, Li X, Xu HE, Yin W
Cell Rep (2024) 43 pp. 114511-114511 [ DOI: doi:10.1016/j.celrep.2024.114511 Pubmed: 39024101 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Bombesin receptor subtype-3 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Ba1 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Ba1-brs3-gq complex (Complex from Homo sapiens)
Cryo-EM structure of the MK-5046-bound BRS3-Gq complex
Li C, Xu Y, Su W, He X, Li J, Li X, Xu HE, Yin W
Cell Rep (2024) 43 pp. 114511-114511 [ DOI: doi:10.1016/j.celrep.2024.114511 Pubmed: 39024101 ]
- (2~{s})-1,1,1-tris(fluoranyl)-2-(4-pyrazol-1-ylphenyl)-3-[5-[[1-(trifluoromethyl)cyclopropyl]methyl]-1~{h}-imidazol-2-yl]propan-2-ol (444 Da, Ligand)
- Mk-5046-brs3-gq complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Bombesin receptor subtype-3 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody nb35 (16 kDa, Protein from Lama glama)
- Scfv16 (26 kDa, Protein from Mus musculus)
cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 1
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8jpb
Q-score: 0.448
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Nts(8-13) (819 Da, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Staurosporine (466 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Ntsr1-grk2-galpha(q) complexes 1 (Complex from Homo sapiens)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Tetrafluoroaluminate ion (102 Da, Ligand)
cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 2
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8jpc
Q-score: 0.458
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Nts(8-13) (819 Da, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Staurosporine (466 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Ntsr1-grk2-galpha(q) complexes 2 (Complex from Homo sapiens)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Tetrafluoroaluminate ion (102 Da, Ligand)
Focused refinement structure of GRK2 in NTSR1-GRK2-Galpha(q) complexes
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8jpd
Q-score: 0.497
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Focused refinement structure of grk2 in ntsr1-grk2-galpha(q) complexes (Complex from Bos taurus)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Staurosporine (466 Da, Ligand)
Focused refinement structure of Galpha(q) in NTSR1-GRK2-Galpha(q) complexes
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8jpe
Q-score: 0.447
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Focused refinement structure of galpha(q) in ntsr1-grk2-galpha(q) complexes (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
Focused refiment structure of NTSR1 in NTSR1-GRK2-Galpha(q) complexes
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8jpf
Q-score: 0.479
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Focused refiment structure of ntsr1 in ntsr1-grk2-galpha(q) complexes (Complex from Homo sapiens)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
- Nts (819 Da, Protein from synthetic construct)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
Structure of A2BR bound to synthetic agonists BAY 60-6583
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8hdo
Q-score: 0.497
Cai H, Xu Y, Guo S, He X, Sun J, Li X, Li C, Yin W, Cheng X, Jiang H, Xu HE, Xie X, Jiang Y
Cell Discov (2022) 8 pp. 140-140 [ Pubmed: 36575181 DOI: doi:10.1038/s41421-022-00503-1 ]
- Nanobody 35 (13 kDa, Protein from Camelus bactrianus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- A2br-g protein-bay 60-6583 complex (160 kDa, Complex from Homo sapiens)
- Adenosine a2b receptor (36 kDa, Protein from Homo sapiens)
- 2-[6-azanyl-3,5-dicyano-4-[4-(cyclopropylmethoxy)phenyl]pyridin-2-yl]sulfanylethanamide (379 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Chimeric minigs (29 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Structure of A2BR bound to endogenous agonists adenosine
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8hdp
Q-score: 0.428
Cai H, Xu Y, Guo S, He X, Sun J, Li X, Li C, Yin W, Cheng X, Jiang H, Xu HE, Xie X, Jiang Y
Cell Discov (2022) 8 pp. 140-140 [ Pubmed: 36575181 DOI: doi:10.1038/s41421-022-00503-1 ]
- Nanobody 35 (13 kDa, Protein from Camelus bactrianus)
- A2br-g protein-adenosine complex (160 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Adenosine a2b receptor (36 kDa, Protein from Homo sapiens)
- Adenosine (267 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Chimeric minigs (29 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)