Cryo-EM structure of the Cas9d-sgRNA-target DNA complex
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 8zdr
Q-score: 0.541
To Be Published
- A protein (Complex from metagenome)
- Zinc ion (65 Da, Ligand)
- Dna (32-mer) (11 kDa, DNA from metagenome)
- Dna (5'-d(p*cp*gp*gp*gp*tp*cp*ap*ap*ap*tp*g)-3') (11 kDa, DNA from metagenome)
- Rna (144-mer) (51 kDa, RNA from metagenome)
- A protein (86 kDa, Protein from metagenome)
Cryo-EM structure of the Cas9d-sgRNA complex
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8zq9
Q-score: 0.47
To Be Published
- A protein (Complex from metagenome)
- Zinc ion (65 Da, Ligand)
- Rna (128-mer) (51 kDa, RNA from metagenome)
- A protein (86 kDa, Protein from metagenome)
Cryo-EM reveals cholesterol binding in the lysosomal GPCR-like protein LYCHOS
Zhao J, Shen Q, Yong X, Li X, Tian X, Sun S, Xu Z, Zhang X, Zhang L, Yang H, Shao Z, Xu H, Jiang Y, Zhang Y, Yan W
Nat Struct Mol Biol (2025) [ Pubmed: 39824976 DOI: doi:10.1038/s41594-024-01470-9 ]
- Water (18 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Lysosomal cholesterol signaling protein (93 kDa, Protein from Homo sapiens)
- Cholesterol-bound lychos (Complex from Homo sapiens)
- [(2r)-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] octadecanoate (720 Da, Ligand)
- Cholesterol (386 Da, Ligand)
Cryo-EM Structure of MILI(K852A)-piRNA-target (26-nt)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 9ij4
Q-score: 0.474
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ Pubmed: 39814893 DOI: doi:10.1038/s41586-024-08438-1 ]
- Mili(k852a)-pirna-target (26-nt) (Complex from Homo sapiens)
- Rna (26-mer) (8 kDa, RNA from Homo sapiens)
- Piwi-like protein 2 (109 kDa, Protein from Mus musculus)
- Magnesium ion (24 Da, Ligand)
- Rna (25-mer) (8 kDa, RNA from Homo sapiens)
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 9jmz
Q-score: 0.489
Song H, Hao T, Han P, Wang H, Zhang X, Li X, Wang Y, Chen J, Li Y, Jin X, Duan X, Zhang W, Bi Y, Jin R, Sun L, Wang N, Gao GF
Cell (2025) [ Pubmed: 39848246 DOI: doi:10.1016/j.cell.2025.01.019 ]
- Human-infecting bovine influenza h5n1 hemagglutinin complexed with avian receptor analog lsta (Complex from Influenza A virus (A/Indonesia/5/2005(H5N1)))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hemagglutinin (57 kDa, Protein from Influenza A virus (A/Indonesia/5/2005(H5N1)))
- N-acetyl-alpha-neuraminic acid (309 Da, Ligand)
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 9jn0
Q-score: 0.518
Curved structure of mPIEZO1-S2472E
Resolution: 4.07 Å
EM Method: Single-particle
Fitted PDBs: 8ixn
Q-score: 0.301
Liu S, Yang X, Chen X, Zhang X, Jiang J, Yuan J, Liu W, Wang L, Zhou H, Wu K, Tian B, Li X, Xiao B
Neuron (2024) [ DOI: doi:10.1016/j.neuron.2024.11.020 Pubmed: 39719701 ]
- (9r,11s)-9-({[(1s)-1-hydroxyhexadecyl]oxy}methyl)-2,2-dimethyl-5,7,10-trioxa-2lambda~5~-aza-6lambda~5~-phosphaoctacosane-6,6,11-triol (767 Da, Ligand)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Piezo-type mechanosensitive ion channel component 1 (292 kDa, Protein from Mus musculus)
- S2472e-curved structure (Complex from Mus musculus)
Flattened structure of mPIEZO1-S2472E
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8ixo
Q-score: 0.445
Liu S, Yang X, Chen X, Zhang X, Jiang J, Yuan J, Liu W, Wang L, Zhou H, Wu K, Tian B, Li X, Xiao B
Neuron (2024) [ DOI: doi:10.1016/j.neuron.2024.11.020 Pubmed: 39719701 ]
- S2472e-intermediate structure (Complex from Mus musculus)
- (9r,11s)-9-({[(1s)-1-hydroxyhexadecyl]oxy}methyl)-2,2-dimethyl-5,7,10-trioxa-2lambda~5~-aza-6lambda~5~-phosphaoctacosane-6,6,11-triol (767 Da, Ligand)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Piezo-type mechanosensitive ion channel component 1 (292 kDa, Protein from Mus musculus)
Resolution: 2.11 Å
EM Method: Single-particle
Fitted PDBs: 8z8z
Q-score: 0.589
Xiong Q, Zhu Z, Li T, Li X, Zhou Z, Chao Y, Yang C, Feng S, Qu Q, Li D
Nat Struct Mol Biol (2025) [ Pubmed: 39824977 DOI: doi:10.1038/s41594-024-01474-5 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lysosomal cholesterol signaling protein,fluorescent protein (127 kDa, Protein from synthetic construct)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (4~{z},7~{z},10~{z},13~{z},16~{z},19~{z})-docosa-4,7,10,13,16,19-hexaenoate (764 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Lychos (127 kDa, Complex from Homo sapiens)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)