The structure of type III CRISPR-associated deaminase in complex cA6 and ATP, fully activated
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8z3p
Q-score: 0.42
Li Y, Li Z, Yan P, Hua C, Kong J, Wu W, Cui Y, Duan Y, Li S, Li G, Ji S, Chen Y, Zhao Y, Yang P, Hu C, Lu M, Chen M, Xiao Y
Science (2024) pp. eadr0393-eadr0393 [ DOI: doi:10.1126/science.adr0393 Pubmed: 39666823 ]
- Adenosine deaminase domain-containing protein (69 kDa, Protein from Limisphaera ngatamarikiensis)
- Zinc ion (65 Da, Ligand)
- Caad:ca6:atp 6:3:6 (Complex from Limisphaera ngatamarikiensis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(p*ap*ap*ap*ap*ap*a)-3') (1 kDa, RNA from Limisphaera ngatamarikiensis)
The structure of type III CRISPR-associated deaminase in complex cA4
Resolution: 2.28 Å
EM Method: Single-particle
Fitted PDBs: 8z3r
Q-score: 0.605
Li Y, Li Z, Yan P, Hua C, Kong J, Wu W, Cui Y, Duan Y, Li S, Li G, Ji S, Chen Y, Zhao Y, Yang P, Hu C, Lu M, Chen M, Xiao Y
Science (2024) pp. eadr0393-eadr0393 [ DOI: doi:10.1126/science.adr0393 Pubmed: 39666823 ]
- Crispr-associated adenosine deaminase (Complex from Limisphaera ngatamarikiensis)
- Zinc ion (65 Da, Ligand)
- Adenosine deaminase domain-containing protein (70 kDa, Protein from Limisphaera ngatamarikiensis)
- 3'-o-[(r)-{[(2s,3as,4s,6s,6as)-6-(6-amino-9h-purin-9-yl)-2-hydroxy-2-oxotetrahydro-2h-2lambda~5~-furo[3,4-d][1,3,2]dioxaphosphol-4-yl]methoxy}(hydroxy)phosphoryl]adenosine (658 Da, Ligand)
The structure of type III CRISPR-associated deaminase in complex 2cA6 and 2ATP, partial activated
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 8z3k
Q-score: 0.425
Li Y, Li Z, Yan P, Hua C, Kong J, Wu W, Cui Y, Duan Y, Li S, Li G, Ji S, Chen Y, Zhao Y, Yang P, Hu C, Lu M, Chen M, Xiao Y
Science (2024) pp. eadr0393-eadr0393 [ DOI: doi:10.1126/science.adr0393 Pubmed: 39666823 ]
- Crispr-associated adenosine deaminase (Complex from Limisphaera ngatamarikiensis)
- Zinc ion (65 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine deaminase domain-containing protein (70 kDa, Protein from Limisphaera ngatamarikiensis)
- Rna (5'-r(p*ap*ap*ap*ap*ap*a)-3') (1 kDa, RNA from Limisphaera ngatamarikiensis)
The structure of type III CRISPR-associated deaminase apo form
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 8z40
Q-score: 0.464
Dimer of SARS-CoV-2 S1 in complex with H18 and R1-32 Fabs
Yan Q, Gao X, Liu B, Hou R, He P, Ma Y, Zhang Y, Zhang Y, Li Z, Chen Q, Wang J, Huang X, Liang H, Zheng H, Yao Y, Chen X, Niu X, He J, Chen L, Zhao J, Xiong X
Nat Commun (2024) 15 pp. 7585-7585 [ DOI: doi:10.1038/s41467-024-51770-3 Pubmed: 39217172 ]
- Heavy chain of h18 fab (25 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Light chain of r1-32 fab (22 kDa, Protein from Homo sapiens)
- Dimer of sars-cov-2 s1 in complex with h18 and r1-32 fabs (Complex from Severe acute respiratory syndrome coronavirus 2)
- H18 fab (Complex from Homo sapiens)
- Light chain of h18 fab (25 kDa, Protein from Homo sapiens)
- Spike glycoprotein (74 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Heavy chain of r1-32 fab (23 kDa, Protein from Homo sapiens)
- Sars-cov-2 s1 (Complex from Severe acute respiratory syndrome coronavirus 2)
- R1-32 fab (Complex from Homo sapiens)
Cryo-EM structure of CRISPR-Csm effector complex from Mycobacterium canettii
Resolution: 3.73 Å
EM Method: Single-particle
Fitted PDBs: 8wfx
Q-score: 0.39
Zhang H, Shi M, Ma X, Liu M, Wang N, Lu Q, Li Z, Zhao Y, Zhao H, Chen H, Zhang H, Jiang T, Ouyang S, Huo Y, Bi L
Int J Biol Macromol (2024) 260 pp. 129331-129331 [ DOI: doi:10.1016/j.ijbiomac.2024.129331 Pubmed: 38218299 ]
- Crispr system cms protein csm2 (15 kDa, Protein from Mycobacterium canettii)
- Csm effector complex (Complex from Mycobacterium canettii)
- Crispr system single-strand-specific deoxyribonuclease cas10/csm1 (subtype iii-a) (91 kDa, Protein from Mycobacterium canettii)
- Crispr system cms protein csm4 (32 kDa, Protein from Mycobacterium canettii)
- Crispr system cms endoribonuclease csm3 (25 kDa, Protein from Mycobacterium canettii)
- Rna (50-mer) (16 kDa, RNA from Mycobacterium canettii)
- Crispr system cms protein csm5 (42 kDa, Protein from Mycobacterium canettii)
Cryo-EM structure of Thogoto virus polymerase in transcription pre-initiation conformation 1
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8z85
Q-score: 0.597
Xue L, Chang T, Li Z, Wang C, Zhao H, Li M, Tang P, Wen X, Yu M, Wu J, Bao X, Wang X, Gong P, He J, Chen X, Xiong X
Nat Commun (2024) 15 pp. 4620-4620 [ DOI: doi:10.1038/s41467-024-48848-3 Pubmed: 38816392 ]
- Rna-directed rna polymerase catalytic subunit (81 kDa, Protein from Thogoto virus (isolate SiAr 126))
- Polymerase acidic protein (71 kDa, Protein from Thogoto virus (isolate SiAr 126))
- Rna (5'-r(*ap*gp*ap*gp*ap*ap*ap*up*cp*ap*ap*gp*gp*cp*ap*gp*up*u)-3') (5 kDa, RNA from Thogoto virus (isolate SiAr 126))
- Rna (5'-r(*gp*ap*cp*up*gp*cp*cp*up*gp*up*up*up*up*up*gp*cp*u)-3') (5 kDa, RNA from Thogoto virus (isolate SiAr 126))
- Cryo-em structure of thogoto virus polymerase in transcription pre-initiation conformation 1 (Complex from Thogoto virus (isolate SiAr 126))
- Polymerase basic protein 2 (94 kDa, Protein from Thogoto virus (isolate SiAr 126))
Cryo-EM structure of Thogoto virus polymerase in transcription pre-initiation conformation 2
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 8z8j
Q-score: 0.515
Xue L, Chang T, Li Z, Wang C, Zhao H, Li M, Tang P, Wen X, Yu M, Wu J, Bao X, Wang X, Gong P, He J, Chen X, Xiong X
Nat Commun (2024) 15 pp. 4620-4620 [ DOI: doi:10.1038/s41467-024-48848-3 Pubmed: 38816392 ]
- Rna-directed rna polymerase catalytic subunit (81 kDa, Protein from Thogoto virus (isolate SiAr 126))
- Polymerase acidic protein (71 kDa, Protein from Thogoto virus (isolate SiAr 126))
- Rna (5'-r(*ap*gp*ap*gp*ap*ap*ap*up*cp*ap*ap*gp*gp*cp*ap*gp*up*u)-3') (5 kDa, RNA from Thogoto virus (isolate SiAr 126))
- Cryo-em structure of thogoto virus polymerase in transcription pre-initiation conformation 2 (Complex from Thogoto virus (isolate SiAr 126))
- Rna (5'-r(*gp*ap*cp*up*gp*cp*cp*up*gp*up*up*up*up*up*gp*cp*u)-3') (5 kDa, RNA from Thogoto virus (isolate SiAr 126))
- Polymerase basic protein 2 (94 kDa, Protein from Thogoto virus (isolate SiAr 126))
Cryo-EM structure of Thogoto virus polymerase in transcription pre-initiation conformation 3
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8z8n
Q-score: 0.532
Xue L, Chang T, Li Z, Wang C, Zhao H, Li M, Tang P, Wen X, Yu M, Wu J, Bao X, Wang X, Gong P, He J, Chen X, Xiong X
Nat Commun (2024) 15 pp. 4620-4620 [ DOI: doi:10.1038/s41467-024-48848-3 Pubmed: 38816392 ]
- Rna-directed rna polymerase catalytic subunit (81 kDa, Protein from Thogoto virus (isolate SiAr 126))
- Polymerase acidic protein (71 kDa, Protein from Thogoto virus (isolate SiAr 126))
- Cryo-em structure of thogoto virus polymerase in transcription pre-initiation conformation 3 (Complex from Thogoto virus (isolate SiAr 126))
- Rna (5'-r(*ap*gp*ap*gp*ap*ap*ap*up*cp*ap*ap*gp*gp*cp*ap*gp*up*u)-3') (5 kDa, RNA from Thogoto virus (isolate SiAr 126))
- Rna (5'-r(*gp*ap*cp*up*gp*cp*cp*up*gp*up*up*up*up*up*gp*cp*u)-3') (5 kDa, RNA from Thogoto virus (isolate SiAr 126))
- Polymerase basic protein 2 (94 kDa, Protein from Thogoto virus (isolate SiAr 126))
Cryo-EM structure of Thogoto virus polymerase in a transcription initiation conformation
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 8z8x
Q-score: 0.525
Xue L, Chang T, Li Z, Wang C, Zhao H, Li M, Tang P, Wen X, Yu M, Wu J, Bao X, Wang X, Gong P, He J, Chen X, Xiong X
Nat Commun (2024) 15 pp. 4620-4620 [ DOI: doi:10.1038/s41467-024-48848-3 Pubmed: 38816392 ]
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Rna-directed rna polymerase catalytic subunit (81 kDa, Protein from Thogoto virus (isolate SiAr 126))
- Polymerase acidic protein (71 kDa, Protein from Thogoto virus (isolate SiAr 126))
- Rna (5'-r(*ap*gp*ap*gp*ap*ap*ap*up*cp*ap*ap*gp*gp*cp*ap*gp*up*u)-3') (5 kDa, RNA from Thogoto virus (isolate SiAr 126))
- Rna (5'-r(*gp*ap*cp*up*gp*cp*cp*up*gp*up*up*up*up*up*gp*cp*u)-3') (5 kDa, RNA from Thogoto virus (isolate SiAr 126))
- Polymerase basic protein 2 (94 kDa, Protein from Thogoto virus (isolate SiAr 126))
- Cryo-em structure of thogoto virus polymerase in a transcription initiation conformation (Complex from Thogoto virus (isolate SiAr 126))