Cryo-EM structure of the tetrameric SPARSA gRNA-ssDNA complex
Resolution: 3.38 Å
EM Method: Single-particle
Fitted PDBs: 8y80
Q-score: 0.331
Cui N, Zhang JT, Li Z, Wei XY, Wang J, Jia N
Nat Commun (2024) 15 pp. 8610-8610 [ Pubmed: 39366953 DOI: doi:10.1038/s41467-024-52910-5 ]
- Tetrameric sparsa grna-ssdna complex (Complex from Geobacter sulfurreducens)
- Rna (21-mer) (6 kDa, RNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- Piwi domain protein (53 kDa, Protein from Geobacter sulfurreducens)
- Sir2 superfamily protein (66 kDa, Protein from Geobacter sulfurreducens)
- Magnesium ion (24 Da, Ligand)
- Dna (25-mer) (7 kDa, DNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
Cryo-EM structure of the tetrameric SPARSA gRNA-ssDNA-NAD+ complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8y82
Q-score: 0.412
Cui N, Zhang JT, Li Z, Wei XY, Wang J, Jia N
Nat Commun (2024) 15 pp. 8610-8610 [ Pubmed: 39366953 DOI: doi:10.1038/s41467-024-52910-5 ]
- Rna (21-mer) (6 kDa, RNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- Piwi domain protein (53 kDa, Protein from Geobacter sulfurreducens)
- Sir2 superfamily protein (66 kDa, Protein from Geobacter sulfurreducens)
- Tetrameric sparsagrna-ssdna-nad+ complex (Complex from Geobacter sulfurreducens)
- Magnesium ion (24 Da, Ligand)
- Adenosine monophosphate (347 Da, Ligand)
- Dna (25-mer) (7 kDa, DNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
Cryo-EM structure of DSR2-tube complex
Resolution: 3.62 Å
EM Method: Single-particle
Fitted PDBs: 8wya
Q-score: 0.011
Zhang JT, Liu XY, Li Z, Wei XY, Song XY, Cui N, Zhong J, Li H, Jia N
Nat Commun (2024) 15 pp. 2797-2797 [ DOI: doi:10.1038/s41467-024-47177-9 Pubmed: 38555355 ]
- Sir2 family protein (118 kDa, Protein from Bacillus subtilis)
- Bacillus phage spbeta tube protein (29 kDa, Protein from Bacillus phage SPBc2)
- Cryoem structure of dsr2-tube complex (Complex from Bacillus subtilis)
Cryo-EM structure of DSR2 (H171A)-tube-NAD+ complex
Resolution: 3.37 Å
EM Method: Single-particle
Fitted PDBs: 8wyb
Q-score: 0.334
Zhang JT, Liu XY, Li Z, Wei XY, Song XY, Cui N, Zhong J, Li H, Jia N
Nat Commun (2024) 15 pp. 2797-2797 [ DOI: doi:10.1038/s41467-024-47177-9 Pubmed: 38555355 ]
- Bacillus phage spr tube protein (29 kDa, Protein from Bacillus phage SPR)
- Sir2-like domain-containing protein (118 kDa, Protein from Bacillus subtilis)
- Cryoem structure of dsr2 (h171a)-tube-nad+ complex (Complex from Bacillus subtilis)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
Cryo-EM structure of DSR2 (H171A)-tube-NAD+ (partial) complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8wyc
Q-score: 0.547
Zhang JT, Liu XY, Li Z, Wei XY, Song XY, Cui N, Zhong J, Li H, Jia N
Nat Commun (2024) 15 pp. 2797-2797 [ DOI: doi:10.1038/s41467-024-47177-9 Pubmed: 38555355 ]
- Cryoem structure of dsr2 (h171a)-tube nad+ (half) complex (Complex from Bacillus subtilis)
- Bacillus phage spr tube protein (29 kDa, Protein from Bacillus phage SPR)
- Sir2-like domain-containing protein (118 kDa, Protein from Bacillus subtilis)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
Cryo-EM structure of DSR2-DSAD1 complex
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8wyd
Q-score: 0.352
Zhang JT, Liu XY, Li Z, Wei XY, Song XY, Cui N, Zhong J, Li H, Jia N
Nat Commun (2024) 15 pp. 2797-2797 [ DOI: doi:10.1038/s41467-024-47177-9 Pubmed: 38555355 ]
- Cryoem structure of dsr2-dsad1 complex (Complex from Bacillus subtilis)
- Sir2 family protein (118 kDa, Protein from Bacillus subtilis)
- Bacillus phage spbeta dsad1 protein (16 kDa, Protein from Bacillus phage SPBc2)