Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 5.5
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8i47
Q-score: 0.519
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
- Water (18 Da, Ligand)
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in closed state
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8i48
Q-score: 0.547
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 2.5
Resolution: 2.6476 Å
EM Method: Single-particle
Fitted PDBs: 8jj3
Q-score: 0.624
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in intermediate state
Resolution: 3.35 Å
EM Method: Single-particle
Fitted PDBs: 8wcq
Q-score: 0.378
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
- Chloride ion (35 Da, Ligand)
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in open state
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8wcr
Q-score: 0.625
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)
Cryo-EM structure of nanodisc (asolectin) reconstituted GLIC at pH 7.5
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 8i41
Q-score: 0.467
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 7.5
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8i42
Q-score: 0.503
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]