Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7wqw
Q-score: 0.412
Yang X, Ding Z, Peng L, Song Q, Zhang D, Cui F, Xia C, Li K, Yin H, Li S, Li Z, Huang H
Nat Commun (2022) 13 pp. 6955-6955 [ DOI: doi:10.1038/s41467-022-34364-9 Pubmed: 36376282 ]
- Enteropeptidase non-catalytic heavy chain (28 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Active-ep (Complex from Homo sapiens)
- Enteropeptidase catalytic light chain (26 kDa, Protein from Homo sapiens)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7wqx
Q-score: 0.348
Yang X, Ding Z, Peng L, Song Q, Zhang D, Cui F, Xia C, Li K, Yin H, Li S, Li Z, Huang H
Nat Commun (2022) 13 pp. 6955-6955 [ DOI: doi:10.1038/s41467-022-34364-9 Pubmed: 36376282 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Enteropeptidase (55 kDa, Protein from Homo sapiens)
- Inactive-ep (Complex from Homo sapiens)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7wqz
Q-score: 0.347
Yang X, Ding Z, Peng L, Song Q, Zhang D, Cui F, Xia C, Li K, Yin H, Li S, Li Z, Huang H
Nat Commun (2022) 13 pp. 6955-6955 [ DOI: doi:10.1038/s41467-022-34364-9 Pubmed: 36376282 ]
- Active-mutep (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Enteropeptidase catalytic light chain (26 kDa, Protein from Homo sapiens)
- Enteropeptidase non-catalytic heavy chain (28 kDa, Protein from Homo sapiens)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7wr7
Q-score: 0.427
Yang X, Ding Z, Peng L, Song Q, Zhang D, Cui F, Xia C, Li K, Yin H, Li S, Li Z, Huang H
Nat Commun (2022) 13 pp. 6955-6955 [ DOI: doi:10.1038/s41467-022-34364-9 Pubmed: 36376282 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 4-carbamimidamidobenzoic acid (179 Da, Ligand)
- Inhibited-ep (Complex from Homo sapiens)
- Enteropeptidase non-catalytic heavy chain (28 kDa, Protein from Homo sapiens)
- Enteropeptidase catalytic light chain (26 kDa, Protein from Homo sapiens)
Cryo-EM structure of Dot1L and H2BK34ub-H3K79Nle nucleosome 1:1 complex
Resolution: 2.57 Å
EM Method: Single-particle
Fitted PDBs: 7xcr
Q-score: 0.571
Ai H, Sun M, Liu A, Sun Z, Liu T, Cao L, Liang L, Qu Q, Li Z, Deng Z, Tong Z, Chu G, Tian X, Deng H, Zhao S, Li JB, Lou Z, Liu L
Nat Chem Biol (2022) 18 pp. 972-980 [ Pubmed: 35739357 DOI: doi:10.1038/s41589-022-01067-7 ]
- Histone h4 (10 kDa, Protein from Homo sapiens)
- Histone h2a (11 kDa, Protein from Homo sapiens)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (37 kDa, Protein from Homo sapiens)
- Dna (146-mer) (45 kDa, DNA from synthetic construct)
- Complex of dot1l(1-416) and h2bk34ub nucleosome (Complex from Homo sapiens)
- Dna (146-mer) (44 kDa, DNA from synthetic construct)
- Histone h2b type 1-k (10 kDa, Protein from Homo sapiens)
- S-adenosylmethionine (398 Da, Ligand)
- Histone domain-containing protein (11 kDa, Protein from synthetic construct)
Cryo-EM structure of Dot1L and H2BK34ub-H3K79Nle nucleosome 2:1 complex
Resolution: 2.72 Å
EM Method: Single-particle
Fitted PDBs: 7xct
Q-score: 0.514
Ai H, Sun M, Liu A, Sun Z, Liu T, Cao L, Liang L, Qu Q, Li Z, Deng Z, Tong Z, Chu G, Tian X, Deng H, Zhao S, Li JB, Lou Z, Liu L
Nat Chem Biol (2022) 18 pp. 972-980 [ Pubmed: 35739357 DOI: doi:10.1038/s41589-022-01067-7 ]
- Dna (145-mer) (44 kDa, DNA from synthetic construct)
- Histone h2a (11 kDa, Protein from Homo sapiens)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- Histone-lysine n-methyltransferase, h3 lysine-79 specific (37 kDa, Protein from Homo sapiens)
- Dna (145-mer) (44 kDa, DNA from synthetic construct)
- Complex of dot1l(1-416) and h2bk34ub 2:1 nucleosome (Complex from Homo sapiens)
- S-adenosylmethionine (398 Da, Ligand)
- Histone h2b type 1-k (10 kDa, Protein from Homo sapiens)
- Histone domain-containing protein (11 kDa, Protein from Homo sapiens)
- Histone h4 (10 kDa, Protein from Homo sapiens)
cryo-EM map of Dot1L and H2BK34ub-H3K79Nle nucleosome complex containing addition map (1:1)
cryo-EM structure of H2BK34ub nucleosome
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 7xd0
Q-score: 0.457
Ai H, Sun M, Liu A, Sun Z, Liu T, Cao L, Liang L, Qu Q, Li Z, Deng Z, Tong Z, Chu G, Tian X, Deng H, Zhao S, Li JB, Lou Z, Liu L
Nat Chem Biol (2022) 18 pp. 972-980 [ Pubmed: 35739357 DOI: doi:10.1038/s41589-022-01067-7 ]
- Ubiquitin-60s ribosomal protein l40 (8 kDa, Protein from synthetic construct)
- Histone h2a (11 kDa, Protein from Homo sapiens)
- Histone domain-containing protein (11 kDa, Protein from Homo sapiens)
- Histone h2b type 1-k (10 kDa, Protein from synthetic construct)
- H2bk34ub nucleosome (Complex from Homo sapiens)
- Dna (146-mer) (45 kDa, DNA from synthetic construct)
- Histone h4 (9 kDa, Protein from Homo sapiens)
- Dna (146-mer) (44 kDa, DNA from synthetic construct)