Cryo-EM structure of SUCR1 in complex with succinate and Gi protein
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 8wog
Q-score: 0.556
Cell Rep (2024) 43 pp. 114381-114381 [ DOI: doi:10.1016/j.celrep.2024.114381 Pubmed: 38923454 ]
- Cryo-em structure of sucr1 in complex with succinate and gi protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Succinate receptor 1 (35 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Succinic acid (118 Da, Ligand)
- Water (18 Da, Ligand)
Cryo-EM structure of SUCR1 in complex with cis-epoxysuccinic acid and Gi proteins
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 8wp1
Q-score: 0.555
Cell Rep (2024) 43 pp. 114381-114381 [ Pubmed: 38923454 DOI: doi:10.1016/j.celrep.2024.114381 ]
- Cryo-em structure of sucr1 in complex with cis-epoxysuccinic acid and gi proteins (Complex from Homo sapiens)
- (2r,3s)-oxirane-2,3-dicarboxylic acid (132 Da, Ligand)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Succinate receptor 1 (35 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
To Be Published
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Gpr1-c9-gi-scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Chemerin-like receptor 2 (34 kDa, Protein from Homo sapiens)
- Retinoic acid receptor responder protein 2 (1 kDa, Protein from Homo sapiens)
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 8k9e
Q-score: 0.548
Xin J, Min Z, Yu L, Yuan X, Liu A, Wu W, Zhang X, He H, Wu J, Xin Y, Blankenship RE, Tian C, Xu X
Plant Cell (2024) [ Pubmed: 38299372 DOI: doi:10.1093/plcell/koae029 ]
- Fe3-s4 cluster (295 Da, Ligand)
- Cytochrome c domain-containing protein (23 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Fe-s-cluster-containing hydrogenase components 1-like protein (113 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Polysulphide reductase nrfd (55 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Unknown (4 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Alternative complex iii (Complex from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- [(2s)-2-octadecanoyloxypropyl] octadecanoate (609 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- 1,3-bis(13-methyltetradecanoyloxy)propan-2-yl pentadecanoate (765 Da, Ligand)
- [(2~{r})-3-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-2-pentadecanoyloxy-propyl] pentadecanoate (663 Da, Ligand)
- Quinol:cytochrome c oxidoreductase membrane protein (19 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Heme c (618 Da, Ligand)
- Cytochrome c7-like domain-containing protein (25 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Uncharacterized protein (12 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- [(2~{r})-3-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-2-tetradecanoyloxy-propyl] hexadecanoate (663 Da, Ligand)
- Quinol:cytochrome c oxidoreductase quinone-binding subunit 2 (45 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8k9f
Q-score: 0.527
Xin J, Min Z, Yu L, Yuan X, Liu A, Wu W, Zhang X, He H, Wu J, Xin Y, Blankenship RE, Tian C, Xu X
Plant Cell (2024) [ Pubmed: 38299372 DOI: doi:10.1093/plcell/koae029 ]
- Fe3-s4 cluster (295 Da, Ligand)
- Cytochrome c domain-containing protein (23 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Fe-s-cluster-containing hydrogenase components 1-like protein (113 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Polysulphide reductase nrfd (55 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Unknown (4 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Alternative complex iii (Complex from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Hypothetical protein (12 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- 1,3-bis(13-methyltetradecanoyloxy)propan-2-yl pentadecanoate (765 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- [(2~{r})-3-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-2-pentadecanoyloxy-propyl] pentadecanoate (663 Da, Ligand)
- Quinol:cytochrome c oxidoreductase membrane protein (19 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Heme c (618 Da, Ligand)
- Cytochrome c7-like domain-containing protein (25 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Iron/sulfur cluster (351 Da, Ligand)
- [(2~{r})-3-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-2-tetradecanoyloxy-propyl] hexadecanoate (663 Da, Ligand)
- Quinol:cytochrome c oxidoreductase quinone-binding subunit 2 (45 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8x2j
Q-score: 0.561
Xin J, Min Z, Yu L, Yuan X, Liu A, Wu W, Zhang X, He H, Wu J, Xin Y, Blankenship RE, Tian C, Xu X
Plant Cell (2024) [ Pubmed: 38299372 DOI: doi:10.1093/plcell/koae029 ]
- 2-heptyl-4-hydroxy quinoline n-oxide (259 Da, Ligand)
- Fe3-s4 cluster (295 Da, Ligand)
- Cytochrome c domain-containing protein (23 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Fe-s-cluster-containing hydrogenase components 1-like protein (113 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Polysulphide reductase nrfd (55 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Unknown (4 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Alternative complex iii (Complex from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Iron/sulfur cluster (351 Da, Ligand)
- 1,3-bis(13-methyltetradecanoyloxy)propan-2-yl pentadecanoate (765 Da, Ligand)
- [(2~{r})-3-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-2-pentadecanoyloxy-propyl] pentadecanoate (663 Da, Ligand)
- Quinol:cytochrome c oxidoreductase membrane protein (19 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Heme c (618 Da, Ligand)
- Cytochrome c7-like domain-containing protein (25 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- Uncharacterized protein (12 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
- [(2~{r})-3-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-2-tetradecanoyloxy-propyl] hexadecanoate (663 Da, Ligand)
- Quinol:cytochrome c oxidoreductase quinone-binding subunit 2 (45 kDa, Protein from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl))
CXCR3-DNGi complex activated by CXCL11
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 8hnk
Q-score: 0.532
Jiao H, Pang B, Liu A, Chen Q, Pan Q, Wang X, Xu Y, Chiang YC, Ren R, Hu H
Nat Struct Mol Biol (2024) 31 pp. 610-620 [ Pubmed: 38177682 DOI: doi:10.1038/s41594-023-01175-5 ]
- Soluble cytochrome b562,c-x-c chemokine receptor type 3,oplophorus-luciferin 2-monooxygenase catalytic subunit (72 kDa, Protein from Oplophorus gracilirostris)
- Cxcr3-cxcl11-dngi-scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- C-x-c motif chemokine 11 (11 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (42 kDa, Protein from Homo sapiens)
- Single-chain variable fragment scfv16 (27 kDa, Protein from Homo sapiens)
CXCR3-DNGi complex activated by PS372424
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8hnl
Q-score: 0.548
Jiao H, Pang B, Liu A, Chen Q, Pan Q, Wang X, Xu Y, Chiang YC, Ren R, Hu H
Nat Struct Mol Biol (2024) 31 pp. 610-620 [ Pubmed: 38177682 DOI: doi:10.1038/s41594-023-01175-5 ]
- Soluble cytochrome b562,c-x-c chemokine receptor type 3,oplophorus-luciferin 2-monooxygenase catalytic subunit (72 kDa, Protein from Oplophorus gracilirostris)
- Cxcr3-ps372424-dngi-scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- (3s)-n-[(2s)-5-carbamimidamido-1-(cyclohexylmethylamino)-1-oxidanylidene-pentan-2-yl]-2-(4-oxidanylidene-4-phenyl-butanoyl)-3,4-dihydro-1h-isoquinoline-3-carboxamide (588 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (42 kDa, Protein from Homo sapiens)
- Single-chain variable fragment scfv16 (27 kDa, Protein from Homo sapiens)
CXCR3-DNGi complex activated by VUF11222
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8hnm
Q-score: 0.546
Jiao H, Pang B, Liu A, Chen Q, Pan Q, Wang X, Xu Y, Chiang YC, Ren R, Hu H
Nat Struct Mol Biol (2024) 31 pp. 610-620 [ Pubmed: 38177682 DOI: doi:10.1038/s41594-023-01175-5 ]
- Soluble cytochrome b562,c-x-c chemokine receptor type 3,oplophorus-luciferin 2-monooxygenase catalytic subunit (72 kDa, Protein from Oplophorus gracilirostris)
- Cxcr3-vuf11222-dngi-scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- [4-(2-bromophenyl)phenyl]methyl-[[(1r,5s)-6,6-dimethyl-2-bicyclo[3.1.1]hept-2-enyl]methyl]-dimethyl-azanium (425 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (42 kDa, Protein from Homo sapiens)
- Single-chain variable fragment scfv16 (27 kDa, Protein from Homo sapiens)
Structure of CXCR3 complexed with antagonist SCH546738
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8hnn
Q-score: 0.434
Jiao H, Pang B, Liu A, Chen Q, Pan Q, Wang X, Xu Y, Chiang YC, Ren R, Hu H
Nat Struct Mol Biol (2024) 31 pp. 610-620 [ Pubmed: 38177682 DOI: doi:10.1038/s41594-023-01175-5 ]
- Soluble cytochrome b562,c-x-c chemokine receptor type 3,kappa-type opioid receptor (56 kDa, Protein from Homo sapiens)
- Nb6 (18 kDa, Protein from Lama glama)
- Cholesterol (386 Da, Ligand)
- Cxcr3-kor-sch546738-nb6 (Complex from Homo sapiens)
- 3-azanyl-6-chloranyl-5-[(3s)-4-[1-[(4-chlorophenyl)methyl]piperidin-4-yl]-3-ethyl-piperazin-1-yl]pyrazine-2-carboxamide (492 Da, Ligand)